A combined use of Chondrocytes Micro Grafts (CMG) Mixed with Platelet Rich Plasma (PRP) in Patients Affected by Pinch Nose Deformity

In Vitro and In Vivo Studies of Alar-Nasal Cartilage Using Autologous Micro-Grafts: The Use of the Rigenera® Protocol in the Treatment of an Osteochondral Lesion of the Nose

“…This construct, subcutaneously injected, resulted in a persistent cartilage tissue with appropriate morphology, adequate central nutritional perfusion without central necrosis or ossification, and further augmented nasal dorsum without obvious contraction and deformation. In addition, microscopic analysis of excisional fragments showed the persistence of healthy cartilage tissue with the formation of new capillaries penetrating into the cartilage as previously reported [29,30]. Here, we demonstrated that the Rigenera ® System is a useful method to isolate human chondrocytes when cells were injected with PRP in vivo in patients affected by nasal valve collapse and pinched nose deformity or cartilage defects.…”

“…Chondrocytes obtained were suitable to form autologous micro-grafts, which could be used alone or in combination with Platelet Rich Plasma (PRP) as published [29,30], and as reported in a patient analyzed in this work, to obtain a biocomplex ready to be implantable in subjects in need of such intervention. PRP was prepared with the approval of the transfusional service from a small volume of blood.…”

“…Using the Rigenera ® protocol, in just one surgical time, the patient is the donor and the acceptor of calibrated micro-grafts. Several authors demonstrated that: (i) Rigenera ® improves the healing of bone lesions as suggested by the use of micro-grafts obtained by periosteum in combination with collagen sponge [36]; (ii) Rigenera ® is effective in the process of wound healing, improving the superficialization of the wound and reducing wound size [25]; (iii) Rigenera ® opens new surgical strategies for rhinoplasty [29,30]. In this manuscript, we demonstrated with in vitro tests that micro-grafts isolated from murine and human auricular cartilage consisted of living single cells and clusters with mesenchymal properties.…”

Progenitor‐cell‐enriched micrografts as a novel option for the management of androgenetic alopecia

“…In addition, the regenerative potential of the micrografts and the clinical efficacy have been already shown for bone regeneration (Brunelli et al, 2013;Rodriguez et al, 2017), in the treatment of ulcers (De Francesco et al, 2017;Miranda, Farina, & Farina, 2018;Trovato, Failla, Serantoni, & Palumbo, 2016), wound dehiscences (Baglioni, Trovato, Marcarelli, Frenello, & Bocchiotti, 2016;Marcarelli, Trovato, Novarese, Riccio, & Graziano, 2017), pathological scars (Svolacchia, De Francesco, Trovato, Graziano, & Ferraro, 2016), and lastly for cartilage and cardiac regeneration Lampinen, Nummi, Nieminen, Harjula, & Kankuri, 2017;Gentile, Scioli, Bielli, Orlandi, & Cervelli, 2016).…”