A commentary on current perspectives on<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>, Johne’s disease and Crohn’s disease: A review by Over et al. (2011)

Robertson, Ros; Hill, Bruce; Cerf, O.; Jordan, Kieran; Venter, Pierre

doi:10.3109/1040841x.2011.649712

Cited by 9 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PTB is responsible for significant economic losses in dairy herds worldwide due to decreased milk production, increased management costs and premature culling from clinical disease 6–9 . Furthermore, there is some evidence suggesting that MAP infection might be associated with Crohn’s disease (CD) in humans 10 . Several studies have demonstrated that more than 50% of the dairy cattle herds are positive for MAP antibodies in USA and in Europe and, therefore, bovine PTB can be considered endemic in these areas 11,12 .…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway

Alonso‐Hearn

Canive

Blanco‐Vázquez

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Paratuberculosis is chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Whole RNA-sequencing (RNA-Seq) is a promising source of novel biomarkers for early MAP infection and disease progression in cattle. Since the blood transcriptome is widely used as a source of biomarkers, we analyzed whether it recapitulates, at least in part, the transcriptome of the ileocecal valve (ICV), the primary site of MAP colonization. Total RNA was prepared from peripheral blood (PB) and ICV samples, and RNA-Seq was used to compare gene expression between animals with focal or diffuse histopathological lesions in gut tissues versus control animals with no detectable signs of infection. Our results demonstrated both shared, and PB and ICV-specific gene expression in response to a natural MAP infection. As expected, the number of differentially expressed (DE) genes was larger in the ICV than in the PB samples. Among the DE genes in the PB and ICV samples, there were some common genes irrespective of the type of lesion including the C-X-C motif chemokine ligand 8 (CXCL8/IL8), apolipoprotein L (APOLD1), and the interferon inducible protein 27 (IFI27). The biological processes (BP) enriched in the PB gene expression profiles from the cows with diffuse lesions included the killing of cells of other organism, defense response, immune response and the regulation of neutrophil chemotaxis. Two of these BP, the defense and immune response, were also enriched in the ICV from the cows with diffuse lesions. Metabolic analysis of the DE genes revealed that the N-glycan biosynthesis, bile secretion, one-carbon pool by folate and purine metabolism were significantly enriched in the ICV from the cows with focal lesions. In the ICV from cows with diffuse lesions; the valine, leucine and isoleucine degradation route, purine metabolism, vitamin digestion and absorption and the cholesterol routes were enriched. Some of the identified DE genes, BP and metabolic pathways will be studied further to develop novel diagnostic tools, vaccines and immunotherapeutics.

show abstract

Section: Introductionmentioning

confidence: 99%

RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway

Alonso‐Hearn

Canive

Blanco‐Vázquez

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…paratuberculosis (MAP) is a chronic granulomatous enteritis of ruminants, both domestic and wild, including cattle, sheep, deer, and other mammalian species ( 1 ). Furthermore, there is some evidence, albeit contentious, suggesting that infection with MAP may be associated with Crohn’s disease in humans ( 2 – 4 ). While prevalence figures of Johne’s disease in cattle are difficult to determine – due, in part, to limited sensitivity and specificity of MAP diagnostic tests – current estimates in European countries vary from 31 to 71% ( 5 – 8 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Bovine Monocyte-Derived Macrophage Response to Mycobacterium avium Subspecies Paratuberculosis Infection Using RNA-seq

et al. 2015

View full text Add to dashboard Cite

Johne’s disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants with serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed and subvert host macrophage processes, resulting in subclinical infections that can lead to immunopathology and dissemination of disease. Analysis of the host macrophage transcriptome during infection can therefore shed light on the molecular mechanisms and host-pathogen interplay associated with Johne’s disease. Here, we describe results of an in vitro study of the bovine monocyte-derived macrophage (MDM) transcriptome response during MAP infection using RNA-seq. MDM were obtained from seven age- and sex-matched Holstein-Friesian cattle and were infected with MAP across a 6-h infection time course with non-infected controls. We observed 245 and 574 differentially expressed (DE) genes in MAP-infected versus non-infected control samples (adjusted P value ≤0.05) at 2 and 6 h post-infection, respectively. Functional analyses of these DE genes, including biological pathway enrichment, highlighted potential functional roles for genes that have not been previously described in the host response to infection with MAP bacilli. In addition, differential expression of pro- and anti-inflammatory cytokine genes, such as those associated with the IL-10 signaling pathway, and other immune-related genes that encode proteins involved in the bovine macrophage response to MAP infection emphasize the balance between protective host immunity and bacilli survival and proliferation. Systematic comparisons of RNA-seq gene expression results with Affymetrix® microarray data generated from the same experimental samples also demonstrated that RNA-seq represents a superior technology for studying host transcriptional responses to intracellular infection.

show abstract

Bovine Paratuberculosis and Human Crohn’s Disease: Is There a Zoonotic Linkage?

Hobmaier¹,

Neuendorf²,

Ackermann³

2023

Zoonoses: Infections Affecting Humans and Animals

View full text Add to dashboard Cite

A commentary on current perspectives onMycobacterium aviumsubsp.paratuberculosis, Johne’s disease and Crohn’s disease: A review by Over et al. (2011)

Cited by 9 publications

References 8 publications

RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway

RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway

Analysis of the Bovine Monocyte-Derived Macrophage Response to Mycobacterium avium Subspecies Paratuberculosis Infection Using RNA-seq

Bovine Paratuberculosis and Human Crohn’s Disease: Is There a Zoonotic Linkage?

Contact Info

Product

Resources

About