A common major surface antigen on amastigotes and promastigotes of Leishmania species.

Colomer-Gould, V.; Quintão, L. G.; Keithly, Jan S.; Nogueira, N

doi:10.1084/jem.162.3.902

Cited by 106 publications

(43 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Gp63 has been described to be important in cell-cell (host-parasite) interaction (30), cell infectivity (19,40), specific diagnosis (14), and immunoprotection (31,41). This molecule was also identified in the sera from patients with CL, mucocutaneous leishmaniasis, or VL (7,9,14,20,21,26).…”

Section: Discussionmentioning

confidence: 99%

“…When we evaluated the WB results for the presence of at least one of the diagnostic antigenic bands, the sensitivity was calculated to be 99.1%. The 63-kDa protein was identified as a glycoprotein by staining with periodic acid-Schiff, indicating that it could be the main promastigote surface molecule (molecular mass, 60 to 65 kDa) common to all Leishmania species (5,7,10,12,21). Gp63 has been described to be important in cell-cell (host-parasite) interaction (30), cell infectivity (19,40), specific diagnosis (14), and immunoprotection (31,41).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

Zeyrek

Korkmaz

Özbel

2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

In this study, we aimed to evaluate the validity of the conventional enzyme-linked immunosorbent assay (ELISA) and the Western blotting test for the diagnosis of anthroponotic cutaneous leishmaniasis (ACL) using serum samples obtained from 51 patients with parasitologically proven nontreated CL (NonT-CL patients) and 62 patients under treatment for CL (UT-CL patients). Additionally, 29 serum samples obtained from patients with parasitologically and serologically proven visceral leishmaniasis (VL) were also used as positive controls, and serum samples from 43 blood donors were used as negative controls. All sera were diluted to the same dilution (1/100). Leishmania infantum MON-1 was used as the antigen in the conventional ELISA. The sera of 27 (93.1%) of 29 VL patients were seropositive by ELISA, while the sera of 40 (78.4%) of 51 NonT-CL patients and 43 (69.3%) of 62 UT-CL patients were seropositive by the conventional ELISA. The absorbance values of the CL patients' sera were significantly lower than the absorbance values of the VL patients' sera. Bands between 15 and 118 kDa were detected in two groups of CL patients. Among all bands, the 63-kDa band was found to be more sensitive (88.5%). When we evaluated the Western blotting results for the presence of at least one of the diagnostic antigenic bands, the sensitivity was calculated to be 99.1%. By using serological tests, a measurable antibody response was detected in most of the CL patients in Sanliurfa, Turkey. It is also noted that this response can be changed according to the sizes, types, and numbers of lesions that the patient has. The Western blot test was found to be more sensitive and valid than the conventional ELISA for the serodiagnosis of ACL. In some instances, when it is very difficult to demonstrate the presence of parasites in the smears, immunodiagnosis can be a valuable alternative for the diagnosis of ACL.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

Zeyrek

Korkmaz

Özbel

2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…Little has been done thus far to characterize the molecules that the parasites in these groups may have in common. Of the several proteins and glycoproteins shared by the leishmania that cause human disease, a 63-65-kD surface antigen appears to be highly conserved among these species and subspecies as judged by serological cross-reactivity (7,14,15), and by DNA sequence analysis (our unpublished data). This antigen from L. chagasi appeared at 63-65 kD.…”

Section: Resultsmentioning

confidence: 99%

In vitro responses to Leishmania antigens by lymphocytes from patients with leishmaniasis or Chagas' disease.

Reed¹,

Carvalho²,

Sherbert³

et al. 1990

J. Clin. Invest.

View full text Add to dashboard Cite

T cell responses are correlated with recovery from and resistance to leishmaniasis. Antigens of Leishmania chagasi were evaluated by determining their ability to elicit in vitro proliferation and cytokine production in peripheral blood lymphocytes and in T cell lines and clones from patients with histories of leishmaniasis or Chagas' disease. Antigens tested were selected by their reactivity with patient antibodies. Several of the antigens induced proliferative responses in peripheral blood lymphocytes from patients recovered from visceral or cutaneous leishmaniasis or with chronic Chagas' disease. Two purified glycoproteins, 30 and 42 kD, were consistently among the most effective in eliciting high proliferative responses and IL-2 production. Lymphocytes from a recovered visceral leishmaniasis patient were used to produce T cell lines against either the 30-or 42-kD antigen. Each of the lines responded to both of these antigens as well as to crude leishmania lysate. Cb4+ T cell clones specific for either or both of these antigens were also isolated from a visceral leishmaniasis patient. In contrast, rabbit antisera produced against these two antigens were not crossreactive. Both antigens were effective in inducing the production of IFN-'y from T cell lines from both leishmaniasis and Chagas' disease patients. These studies demonstrate the potential for defining parasite antigens with broad immunostimulatory capabilities. (J. Clin. Invest. 1990. 85:690-696.) leishmania * antigens -T cells * leishmaniasis

show abstract

“…We chose a single Leishmania antigen, gp63, for these studies. gp63 is one of the most abundant molecules on the surface of the parasite (11,24). Previous studies by us and others have shown that this molecule can influence complement fixation and parasite adhesion to macrophages (6,10).…”

Section: Discussionmentioning

confidence: 99%

Vaccination against the Intracellular PathogensLeishmania majorandL. amazonensisby Directing CD40 Ligand to Macrophages

2001

View full text Add to dashboard Cite

CD40 ligand (CD40L) is a potent inducer of interleukin-12 (IL-12) production from macrophages and dendritic cells. We show that combining CD40L with antigen derived from Leishmania is an effective way to preferentially induce type 1 immune responses to the antigen and to vaccinate mice against subsequent challenge with virulent organisms. Mice vaccinated in this way had smaller lesions, with more than 1,000-fold fewer parasites within them. To improve the efficiency of CD40L-induced immunopotentiation, we attempted to specifically direct CD40L to macrophages. We developed transfected cells expressing CD40L and a single Leishmania antigen, gp63. These cells bound efficiently to macrophages and induced robust IL-12 production. Vaccination with these cotransfected cells provided a significant degree of protection against challenge with virulent organisms. CD40L was also adsorbed to the surface of virulent Leishmania. These organisms induced only modest lesions in genetically susceptible mice, and the lesions had an average of 10 5 -fold fewer organisms within them relative to control mice. These studies suggest that CD40L could be exploited to improve vaccines against intracellular pathogens, especially those organisms that reside within cells expressing CD40 on their surface.

show abstract

A common major surface antigen on amastigotes and promastigotes of Leishmania species.

Cited by 106 publications

References 39 publications

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

In vitro responses to Leishmania antigens by lymphocytes from patients with leishmaniasis or Chagas' disease.

Vaccination against the Intracellular PathogensLeishmania majorandL. amazonensisby Directing CD40 Ligand to Macrophages

Contact Info

Product

Resources

About