This study aims to examine the water quality of Lake Sentani using both in-situ data and satellite remote sensing data. In-situ data was taken in July 2023 at 18 sampling sites, including temperature, DO, pH, TDS, and water transparency. In-situ data is also used to develop and validate algorithms for estimating water quality from satellite remote sensing data. Multi-temporal Landsat-8 satellite imagery was used to spatially and temporally map the surface water quality of Lake Sentani. In-situ data showed temperature, DO, pH, TDS, and water transparency ranging from 29.3°C to 31.8°C, 1.7 mg/L to 7.9 mg/L, 7.75 to 8.64, 23 mg/L to 46 mg/L, and 2.28 m to 2.94 m, respectively. Only water transparency does not meet the quality standards for water quality (class 1 and class 2), while the other parameters meet class 1 to class 3 quality standards for surface water samples. The accuracy of the algorithm used and the resulting one has a low Mean Absolute Error value, namely 0.81 (temperature), 0.37 (DO), 4.84 (TDS), and 0.12 (water transparency). The temperature and TDS concentrations from the satellite imagery data ranged from 28.110°C to 33.918°C, and 7.829 mg/L to 102.702 mg/L, respectively. Both of these parameters still meet water quality standards. The DO concentrations ranged from 2.228 mg/L to 12.562 mg/L, and water transparency ranged from 0.424 m to 3.151 m. The concentration of DO and water transparency do not meet quality standards in several parts of Lake Sentani, especially in November and August.