A comparative analysis of characteristic floral scent compounds in <i>Prunus mume</i> and related species

Hao, Ruijie; Du, Dongliang; Wang, Tao; Yang, Weiru; Wang, Jia; Zhang, Qixiang

doi:10.1080/09168451.2014.936346

Cited by 26 publications

(22 citation statements)

References 32 publications

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“…In earlier research, we found that the dominant compound responsible for the fragrance of P. mume flowers was ester benzyl acetate 25 , which is synthesized by the benzenoid/phenylpropanoid pathway. CbBEAT in C. breweri has been reported to catalyze the synthesis of benzyl acetate using benzyl alcohol and acetyl-CoA as substrates, and the substrate benzyl alcohol has been shown to be synthesized from the reduction of benzaldehyde in plants 2,10 .…”

Section: Resultsmentioning

confidence: 99%

Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

et al. 2019

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Prunus mume is the only plant in the genus Prunus of the Rosaceae family with a characteristic floral scent, and the main component of this scent is benzyl acetate. By contrast, benzyl acetate is not synthesized in Prunus persica flowers. Here, we searched for benzyl alcohol acetyltransferase (BEAT) genes based on genomic data from P. mume and P. persica and found 44 unique PmBEATs in P. mume. These genes, which were mainly detected in clusters on chromosomes, originated from gene duplication events during the species evolution of P. mume, and retroduplication and tandem duplication were the two dominant duplication patterns. The genes PmBEAT34, PmBEAT36 and PmBEAT37, which were generated by tandem duplication, were highly expressed in flowers, and their highest levels were detected during the blooming stage. In vitro, PmBEAT34, PmBEAT3, and PmBEAT37 all had benzyl alcohol acetyltransferase activity that was localized in the cytoplasm. Overexpression of the PmBEAT36 or PmBEAT37 genes increased benzyl acetate production in the petal protoplasts of P. mume, and interference in the expression of these genes slightly decreased the benzyl acetate content. In addition, light and temperature regulated the expression of the PmBEAT34, PmBEAT36 and PmBEAT37 genes. According to these results, we hypothesize that the expansion of the PmBEAT genes in the genome induce the characteristic floral scent of P. mume.

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Section: Resultsmentioning

confidence: 99%

Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

et al. 2019

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“…Studies have shown that eugenol is the most important constituent of the characteristic floral scent of P . mume [6]. Eugenol, with the exotic smell of cloves, is among the volatile phenylpropanoid compounds derived from phenylalanine [7].…”

Section: Introductionmentioning

confidence: 99%

“…Most interspecific hybrids between P . mume and other species of Prunus lack this fragrance [6, 14], in which eugenol is a principal component contributing to the floral scent among both emitted and endogenous compounds. To solve this breeding problem, we must understand the molecular mechanism of eugenol production.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterization, expression and enzyme activity analysis of coniferyl alcohol acetyltransferase genes involved in eugenol biosynthesis in Prunus mume

et al. 2019

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Prunus mume, a traditional Chinese flower, is the only species of Prunus known to produce a strong floral fragrance, of which eugenol is one of the principal components. To explore the molecular mechanism of eugenol biosynthesis in P. mume, patterns of dynamic, spatial and temporal variation in eugenol were analysed using GC-MS. Coniferyl alcohol acetyltransferase (CFAT), a member of the BAHD acyltransferase family, catalyses the substrate of coniferyl alcohol to coniferyl acetate, which is an important substrate for synthesizing eugenol. In a genome-wide analysis, we found 90 PmBAHD genes that were phylogenetically clustered into five major groups with motif compositions relatively conserved in each cluster. The phylogenetic tree showed that the PmBAHD67-70 proteins were close to the functional CFATs identified in other species, indicating that these four proteins might function as CFATs. In this work, 2 PmCFAT genes, named PmCFAT1 and PmCFAT2, were cloned from P. mume ‘Sanlunyudie’, which has a strong fragrance. Multiple sequences indicated that PmCFAT1 contained two conserved domains, HxxxD and DFGWG, whereas DFGWG in PmCFAT2 was changed to DFGFG. The expression levels of PmCFAT1 and PmCFAT2 were examined in different flower organs and during the flowering stages of P. mume ‘Sanlunyudie’. The results showed that PmCFAT1 was highly expressed in petals and stamens, and this expression increased from the budding stage to the full bloom stage and decreased in the withering stage, consistent with the patterns of eugenol synthesis and emission. However, the peak of gene expression appeared earlier than those of eugenol synthesis and emission. In addition, the expression level of PmCFAT2 was higher in pistils and sepals than in other organs and decreased from the budding stage to the blooming stage and then increased in the withering stage, which was not consistent with eugenol synthesis. Subcellular localization analysis indicated that PmCFAT1 and PmCFAT2 were located in the cytoplasm and nucleus, while enzyme activity assays showed that PmCFAT1 is involved in eugenol biosynthesis in vitro. Overall, the results suggested that PmCFAT1, but not PmCFAT2, contributed to eugenol synthesis in P. mume.

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“…mume (mei) from Rosaceae, is a famous ornamental and fruit tree blooming in early spring within January and February, and it is mainly cultivated in Yangtze valley that locates in the subtropical monsoon climate zone [12]. It emits recognizable and pleasant scents, making it distinguishable from other species in Prunus, such as P. sibirica, P. armeniaca, and P. persica [13]. It has been reported that the aromatic benzenoid, benzyl acetate, is the main component in its floral volatiles [14].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Floral Scent Compounds in Intraspecific Cultivars of Prunus mume with Different Corolla Colours

et al. 2019

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Prunus mume is the only fragrant flowering species of Prunus. According to the previous studies, benzyl acetate and eugenol dominate its floral scent. However, the diversity of its floral scents remains to be elucidated. In this work, the floral volatiles emitted from eight intraspecific cultivars of P. mume with white, pink and red flowers, were collected and analyzed using headspace solid-phase microextraction combined with gas chromatograms-mass spectrometry (HS-SPME-GC-MS). In total, 31 volatile compounds were identified, in which phenylpropanoids/benzenoids accounted for over 95% of the total emission amounts. Surprisingly, except for benzyl acetate and eugenol, several novel components, such as benzyl alcohol, cinnamyl acohol, cinnamy acetate, and benzyl benzoate were found in some cultivars. The composition of floral volatiles in cultivars with white flowers was similar, in which benzyl acetate was dominant, while within pink flowers, there were differences of floral volatile compositions. Principal component analysis (PCA) showed that the emissions of benzyl alcohol, cinnamyl alcohol, benzyl acetate, eugenol, cinnamyl acetate, and benzyl benzoate could make these intraspecific cultivars distinguishable from each other. Further, hierarchical cluster analysis indicated that cultivars with similar a category and amount of floral compounds were grouped together. Our findings lay a theoretical basis for fragrant plant breeding in P. mume.

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A comparative analysis of characteristic floral scent compounds in Prunus mume and related species

Cited by 26 publications

References 32 publications

Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

Genome-wide identification, characterization, expression and enzyme activity analysis of coniferyl alcohol acetyltransferase genes involved in eugenol biosynthesis in Prunus mume

A Comparative Analysis of Floral Scent Compounds in Intraspecific Cultivars of Prunus mume with Different Corolla Colours

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