A Comparative Analysis of Photobiomodulation-Mediated Biological Effects of Single Versus Double Irradiation on Dental Pulp Stem Cells: An <i>In Vitro</i> Study

Ahmadi, Fatemeh; Dalirsani, Zohreh; Tayarani‐Najaran, Zahra; Ebrahimzadeh‐Bideskan, Alireza; Shafieian, Reyhaneh

doi:10.1089/photob.2021.0134

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Laser irradiation did not lead to cell activation in the single irradiation group, but the multiple irradiation group exhibited an overall tendency for cell proliferation, with the highest levels observed at a low power of 10-30 mW, particularly at 30 mW. A previous study reported that the activation of hDPSCs occurred when irradiation was administered through multiple exposures at a lower power [20]. This is consistent with the results of the present study.…”

Section: Discussionsupporting

confidence: 92%

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

Yarita,

Kitajima,

Morita

et al. 2024

Dentistry Journal

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This study aimed to determine the effect of photobiomodulation therapy induced by semiconductor laser irradiation on human dental pulp stem cell (hDPSC) proliferation and their differentiation into odontoblast-like cells (OLCs). The effects of various semiconductor laser irradiation conditions on hDPSCs were examined. Three groups were evaluated: a single laser irradiation at 6 h post-seeding, multiple laser irradiations up to four times every 4 days after the first dose, and a control with no laser irradiation. The cells were irradiated at 10, 30, and 150 mW using a semiconductor laser. The effect of laser irradiation on hDPSC differentiation into OLCs was also determined. Four groups were evaluated, including co-culture using basic medium and dentin discs, simple culture using OLC differentiation-inducing medium, co-culture using OLC differentiation-inducing medium and dentin discs, and control culture with basic medium. The expression of the nestin, ALP, DSPP, and DMP-1 genes was measured using real-time PCR. The multiple irradiation group irradiated at 30 mW exhibited significantly more cell proliferation than the control. The expression of nestin associated with differentiation into OLCs during each culture period tended to be lower, whereas DSPP and ALP expression was higher compared with that of the control. Multiple laser irradiations at a low power of 30 mW induced significant hDPSC proliferation and might induce differentiation into OLCs.

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Section: Discussionsupporting

confidence: 92%

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

Yarita,

Kitajima,

Morita

et al. 2024

Dentistry Journal

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“…In another study, human gingival broblasts were shown to reach the highest proliferation rate once irradiated at 635nm (4 j/cm 2 ) as compared to those exposed to 660 or 808nm [31]. Recently, we discussed a positive impact of PBM at 3 and 7 j/cm 2 on the viability and proliferation of dental pulp-derived stem cells in vitro [34]. As shown in the current study, higher employed wavelength (808nm) was more able to encourage osteoblasts and endothelial progenitor cells, as con rmed by higher levels of RunX2 and VEGF mRNA expression; while lower employed wavelength (660nm) showed greater capacity in stimulation of broblasts, validated via higher mRNA expression level of COLI.…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Photobiomodulation in Diabetes-Impaired Alveolar Bone healing; A Histomorphometrical and Molecular Study

salari-sedigh

Dalirsani

Shafieian

2022

Preprint

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Introduction: Diabetes mellitus is increasing worldwide. Photobiomodulation is proposed as a therapeutic method in various medical concerns. This study aimed to compare the effects of photobiomodulation at the wavelength of 660, 808, or 660 + 808nm, on alveolar bone healing in diabetic rats. Materials and methods Bilateral maxillary first molars were extracted from diabetic Wistar rats (n = 36). Right-sided sockets were treated by an In-Ga-Al-P laser at 660nm (7.2 j/cm2, 24 s; DM660), Ga-Al-As laser at 808nm (7 j/cm2, 14 s; DM808), or a combination of these two sets (DM-dual) (n = 12). Left sides served as controls. On days 7 or 14, specimens were assigned for histomorphometric or RT-PCR analysis of RunX2, OCN, COL1 and VEGF expression. Results Irradiated sockets of groups DM-808 and DM-dual showed a significant increase in bone tissue and blood vessel establishment as compared to DM-660. Furthermore, group DM-dual exhibited the least amount of fibrosis tissue as compared to the other groups. Conclusion Within the limits of this study, our experimental model suggested photobiomodulation at 808nm, whether in form of single or combined irradiation, imposing a therapeutic influence on diabetic alveolar bone healing.

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Protective effect of dental pulp stem cells' conditioned medium against cisplatin-induced testicular damage in rats

Hokmabadi,

Ranjbar,

Alipour

et al. 2024

Toxicology

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A Comparative Analysis of Photobiomodulation-Mediated Biological Effects of Single Versus Double Irradiation on Dental Pulp Stem Cells: An In Vitro Study

Cited by 7 publications

References 41 publications

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

Effects of Semiconductor Laser Irradiation on Differentiation of Human Dental Pulp Stem Cells in Co-Culture with Dentin

Comparative Investigation of Photobiomodulation in Diabetes-Impaired Alveolar Bone healing; A Histomorphometrical and Molecular Study

Protective effect of dental pulp stem cells' conditioned medium against cisplatin-induced testicular damage in rats

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