A comparative assessment of different techniques for the extraction and characterization of fucoidan from Indian brown seaweeds

Abstract

In brown seaweeds, cell wall polysaccharides are dissolved in hot water, acid, and organic solvents to extract fucoidan. The study looked at 13 Indian brown seaweeds and used three different fucoidan extraction techniques: hot water (HW), hydrochloric acid (HA), and ethylenediaminetetraacetic acid (EDTA). The yield of the HA extraction technique was higher than that of the EDTA and HW extraction techniques. When compared to the EDTA and HA extraction techniques, the HW extraction technique showed the highest fucose and sulfate content. Furthermore, the EDTA extraction technique revealed the highest protein content compared to the HA and HW extraction techniques. Among different fucoidan samples from various extraction techniques, S. cinctum had a high fucose and sulfate content. Therefore, we evaluated S. cinctum using different extraction techniques for FTIR analysis. The absorption areas near 1270 cm-1, 1030 cm-1 and 870 cm-1 in the HW, HA, and EDTA extraction techniques of S. cinctum indicate the presence of sulfate groups. After examining the different fucoidan extraction techniques, the HW extraction technique was shown to be the most effective and ecologically friendly method for the extraction of fucoidan. Studying the effects of different extraction techniques benefits a variety of industries, including the cosmetics, nutraceutical, and pharmaceutical industries.