A comparative assessment ofin vitrocytotoxic activity and phytochemical profiling ofAndrographis nallamalayanaJ.L.Ellis andAndrographis paniculata(Burm. f.) Nees using UPLC-QTOF-MS/MS approach

Goel, Narender; Gajbhiye, Rahul L.; Saha, Moumita; Nagendra, Chennuru; Reddy, A. Madhusudhana; Ravichandiran, V.; Saha, Krishna Das; Jaisankar, Parasuraman

doi:10.1039/d1ra07496b

Cited by 3 publications

(1 citation statement)

References 111 publications

(111 reference statements)

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“…Data demonstrated our formulated mouthwash has an IC 50 value (43.55 mg/mL). The cytotoxicity activity of AP was categorized according to the National Cancer Institute (NCI) guidelines as follows IC 50 ≤ 20 µg/mL is highly toxic, and IC 50 ≥ 500 µg/mL is evaluated as inactive (Goel et al, 2021). Supporting the finding, cytotoxicity analysis of pure compounds (andrographolide,14-deoxyandrographolide, 1 4 -d e o x y -1 2 -h y d r o x y a n d r o g r a p h o l i d e , a n d neoandrographolide) against monocytic leukemia cells (THP-1) resulted to IC 50 values exceeding 100 µg/mL, suggesting the absence of cytotoxic activities (Tan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

EVALUATION OF TOXICITY AND ANTIBACTERIAL ACTIVITIES OF Andrographis paniculata HERBAL MOUTHWASH AGAINST ORAL PATHOGENS

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KUB

MIRAN

et al. 2022

MAB

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Andrographis paniculata (AP) is an ancient herb known for its medicinal and therapeutic values. In this study, we aimed to evaluate the antibacterial activity and cytotoxicity of AP herbal mouthwash. Aqueous extract of AP was used to prepare the herbal mouthwash. The product was tested against selected oral pathogens namely Actinomyeces viscosus, Staphylococcus aureus, Streptococcus mutans, Streptococcus sobrinus, and Porphyromonas gingivalis for its antibacterial activity using the agar well diffusion method. Toxicity analysis was carried out and subjected to cytotoxicity screening using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, in vivo study using brine shrimp lethality bioassay, and detection of heavy metals using atomic absorption spectroscopy (AAS). Five AP herbal mouthwash concentrations (62.5, 125, 250, 500, 1000 mg/mL) were developed. AP herbal mouthwash exhibited the strongest antibacterial activity against A. viscosus. AP herbal mouthwash (125 to 1000 mg/mL) showed inhibition against P. gingivalis, S. mutans and S. sobrinus, except for S. aureus which did not reflect any sign of antibacterial activity. In brine shrimp lethality bioassay, AP herbal mouthwash has LC50 3255.064 µg/mL. In vitro cytotoxic evaluation was done on L929 mouse fibroblast cell lines using MTT assay with IC50 43.55 mg/mL denotes the mouthwash is non-toxic. Heavy metals content in AP herbal mouthwash falls within the permissible range of plumbum (2.50 ppm) followed by arsenic (1.875 ppm), mercury (0.15 ppm), and cadmium (0.06 ppm). Thus, verifying AP herbal mouthwash is effective in common oral pathogens and has a non-toxic effect.

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Section: Discussionmentioning

confidence: 99%

EVALUATION OF TOXICITY AND ANTIBACTERIAL ACTIVITIES OF Andrographis paniculata HERBAL MOUTHWASH AGAINST ORAL PATHOGENS

ALOJID

KUB

MIRAN

et al. 2022

MAB

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In-vitro and in-silico cholinesterase inhibitory activity of bioactive molecules isolated from the leaves of Andrographis nallamalayana J.L. Ellis and roots of Andrographis beddomei C.B. Clarke

Goel,

Garg,

Nagendra

et al. 2024

Journal of Molecular Structure

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Virtual screening of natural products inspired in-house library to discover potential lead molecules against the SARS-CoV-2 main protease

Garg

Goel

Abhinav

et al. 2022

Journal of Biomolecular Structure and Dynamics

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A comparative assessment ofin vitrocytotoxic activity and phytochemical profiling ofAndrographis nallamalayanaJ.L.Ellis andAndrographis paniculata(Burm. f.) Nees using UPLC-QTOF-MS/MS approach

Abstract: A comparative study of two Andrographis species have been done, and it was found that Andrographis nallamalayana J.L.Ellis is phytochemically and biologically different from Andrographis paniculata (Burm. f.) Nees.

Cited by 3 publications

References 111 publications

EVALUATION OF TOXICITY AND ANTIBACTERIAL ACTIVITIES OF Andrographis paniculata HERBAL MOUTHWASH AGAINST ORAL PATHOGENS

EVALUATION OF TOXICITY AND ANTIBACTERIAL ACTIVITIES OF Andrographis paniculata HERBAL MOUTHWASH AGAINST ORAL PATHOGENS

In-vitro and in-silico cholinesterase inhibitory activity of bioactive molecules isolated from the leaves of Andrographis nallamalayana J.L. Ellis and roots of Andrographis beddomei C.B. Clarke

Virtual screening of natural products inspired in-house library to discover potential lead molecules against the SARS-CoV-2 main protease

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