A comparative assessment of water-soluble selenium metabolites in commercial selenised yeast supplements by liquid chromatography-electrospray ionisation QTOF-MS

Ward, Patrick; Chadha, Marcus; Connolly, Cathal; Stalcup, Apryll M.; Murphy, Richard

doi:10.1016/j.ijms.2018.10.040

Cited by 15 publications

(13 citation statements)

References 40 publications

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“…In our previous study [ 61 ] using the UHPLC-ESI-Orbitrap-MS/MS method in C. utilis yeast biomass, we found the presence of Se compounds with mass from 195.9 to 376.9 m / z . Ward et al [ 59 ] showed that the commercially available yeast contained Se compounds combined with glutathione, which corroborated the earlier findings of Arnaudquilhem et al [ 21 ]. According to literature data [ 37 ], cell protection against oxidative processes predominantly occurs through the functioning of glutathione as a reducing agent.…”

Section: Resultssupporting

confidence: 77%

“…Their presence in many organisms has been well documented, and a binding site consisting of two neighboring Cys residues was identified [58]; such easily accessible sites are present in many yeast proteins and can scavenge (bind) inorganic Se present in Se-rich growth medium. The speciation analysis of organoselenium metabolites obtained from the biomass of microorganisms depends on extraction conditions and on the stability of the extracted analytes [59]. The characterization of LMW Se (water-soluble species) led to the identification of five glutathione derivatives in the 450-700 Da mass range (Table 4).…”

Section: Se Speciationmentioning

confidence: 99%

“…Torula yeast) supplements, no selenohomolanthionine has been detected [14]. The speciation analysis of organoselenium metabolites obtained from the biomass of microorganisms depends on extraction conditions and on the stability of the extracted analytes [59]. The characterization of LMW Se (water-soluble species) led to the identification of five glutathione derivatives in the 450-700 Da mass range (Table 4).…”

Section: Se Speciationmentioning

confidence: 99%

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Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

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Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.

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Section: Resultssupporting

confidence: 77%

Section: Se Speciationmentioning

confidence: 99%

Section: Se Speciationmentioning

confidence: 99%

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Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

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“…In either case, in a good-quality yeast, selenium is metabolized completely to organic forms and plethora of LMW (<1,000 Da) species, referred to as selenometabolome, are produced. Its detailed characterization is essential for the control of the reproducibility of the fermentation process, variance of different strains, and traceability of the product's origin (Ward et al, 2019 ). Furthermore, as some of the metabolites may have superior beneficial activity (and, on the other hand, others can be toxic), the motivation of the producers to offer a well-characterized unique product is evident.…”

Section: Interest In the Identification Of The Products Of The Selenimentioning

confidence: 99%

“…Indeed, the progress in terms of the identification of the detected compounds has been largely unsatisfactory. Recent studies (Gilbert-López et al, 2017 ; Ward et al, 2019 ) show that only a small fraction of the detected, previously unreported compounds could be identified. This clearly demonstrates the need for analytical methods allowing the large-scale speciation analysis of the Se-rich yeast metabolome.…”

Section: Characterization Of the Se-rich Yeast Metabolome: State-of-tmentioning

confidence: 99%

Potential of Fourier Transform Mass Spectrometry (Orbitrap and Ion Cyclotron Resonance) for Speciation of the Selenium Metabolome in Selenium-Rich Yeast

et al. 2020

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The evolution of the field of element speciation, from the targeted analysis for specific element species toward a global exploratory analysis for the entirety of metal- or metalloid-related compounds present in a biological system (metallomics), requires instrumental techniques with increasing selectivity and sensitivity. The selectivity of hyphenated techniques, combining chromatography, and capillary electrophoresis with element-specific detection (usually inductively coupled plasma mass spectrometry, ICP MS), is often insufficient to discriminate all the species of a given element in a sample. The necessary degree of specificity can be attained by ultrahigh-resolution (R >100,000 in the m/z < 1,000 range for a 1 s scan) mass spectrometry based on the Fourier transformation of an image current of the ions moving in an Orbitrap or an ion cyclotron resonance (ICR) cell. The latest developments, allowing the separate detection of two ions differing by a mass of one electron (0.5 mDa) and the measurement of their masses with a sub-ppm accuracy, make it possible to produce comprehensive lists of the element species present in a biological sample. Moreover, the increasing capacities of multistage fragmentation often allow their de novo identification. This perspective paper critically discusses the potential state-of-the-art of implementation, and challenges in front of FT (Orbitrap and ICR) MS for a large-scale speciation analysis using, as example, the case of the metabolism of selenium by yeast.

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Inorganic and Organic Selenium Speciation of Seleno-Yeasts Used as Feed Additives: New Insights from Elemental Selenium Determination

Hachemi¹,

Cardoso²,

Marco³

et al. 2023

Biol Trace Elem Res

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Seleno-Yeasts (SY) used as feed additives are known to contain different Selenium (Se) species. Seleno-Yeasts has been shown, on previous analytical methods, to contain selenomethionine (SeMet), selenocysteine (SeCys), selenate (SeIV) and selenite (SeVI), and various other organic and inorganic Se forms identified but rarely quantified. A new advanced method has allowed elemental Se (Se0), an inorganic Se species, to be quantified, thereby obtaining better insight into the proportion of inorganic Se in SY products. The study aimed to quantify the Se0 in SY products and assess the proportion of inorganic Se in SY. The Se speciation of 13 fresh commercials SY from different suppliers and batches, was assayed for the total Se, inorganic Se species (SeIV, SeVI and Se0), and organic Se species (SeMet and SeCys). Results on total Se were in line with the expected Se concentrations for all evaluated samples. The proportion of Se present as Se0 ranged from 3.6% to 51.8%. The quantity of Se0 in the SY products, added to SeIV and SeVI, indicated an average proportion of inorganic Se of 14.2% for the 13 analyzed SY products. The proportion of Se as SeMet ranged from 19.0% to 71.8%, (average of 55.8%), and a large variability in the SeMet content was observed. The SeCys content was also variable, with an average of 3.8%, relative to the total Se. In conclusion, advances in the analytical characterization have revealed that SY products can have a significantly high proportion of inorganic Se, which could affect the bioavailability of Se from SY supplements and explain their variable and lower bio-efficacy than pure SeMet supplements, such as hydroxy-selenomethionine.

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A comparative assessment of water-soluble selenium metabolites in commercial selenised yeast supplements by liquid chromatography-electrospray ionisation QTOF-MS

Cited by 15 publications

References 40 publications

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Potential of Fourier Transform Mass Spectrometry (Orbitrap and Ion Cyclotron Resonance) for Speciation of the Selenium Metabolome in Selenium-Rich Yeast

Inorganic and Organic Selenium Speciation of Seleno-Yeasts Used as Feed Additives: New Insights from Elemental Selenium Determination

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