A comparative characterization of laryngeal anatomy in the singing mouse

Smith, Samantha K.; Burkhard, Tracy T.; Phelps, Steve

doi:10.1111/joa.13315

Cited by 9 publications

(6 citation statements)

References 54 publications

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“…The details of all specimens are summarized in Table 1. Fetal specimens examined in this study include stages from CS16 to CS24 (R. pusillus: CS16, 17,18,19,20,21,22,23,24; R. malayanus: CS18, 20.5, 22). Postnatal specimens, all of which were R. pusillus, covered postnatal day 0, 14, 21, 30, 45, and adult.…”

Section: Data Acquisitionmentioning

confidence: 99%

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Development of the hyolaryngeal architecture in horseshoe bats: Insights into the evolution of the pulse generation for laryngeal echolocation

Nojiri,

Takechi,

Furutera

et al. 2023

Preprint

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Background The hyolaryngeal apparatus generates biosonar pulses in the laryngeally echolocating bats. The cartilage and muscles comprising the hyolarynx of laryngeally echolocating bats are morphologically modified compared to those of non-bat mammals, as represented by the hypertrophied intrinsic laryngeal muscle. Despite its crucial contribution to laryngeal echolocation, how the development of the hyolarynx in bats differs from that of other mammals is poorly documented. The genus Rhinolophus is one of the most sophisticated laryngeal echolocators, with the highest pulse frequency in bats. The present study provides the first detailed description of the three-dimensional anatomy and development of the skeleton, cartilage, muscle, and innervation patterns of the hyolaryngeal apparatus in two species of rhinolophid bats using micro-computed tomography images and serial tissue sections and compares them with those of laboratory mice. Furthermore, we measured the peak frequency of the echolocation pulse in active juvenile and adult individuals to correspond to echolocation pulses with hyolaryngeal morphology at each postnatal stage. Results We found that the sagittal crests of the cricoid cartilage separated the dorsal cricoarytenoid muscle in horseshoe bats, indicating that this unique morphology may be required to reinforce the repeated closure movement of the glottis during biosonar pulse emission. We also found that the cricothyroid muscle is ventrally hypertrophied throughout ontogeny, and that the cranial laryngeal nerve has a novel branch supplying the hypertrophied region of this muscle. Our bioacoustic analyses revealed that the peak frequency shows negative allometry against skull growth, and that the volumetric growth of all laryngeal cartilages is correlated with the pulse peak frequency. Conclusions The unique patterns of muscle and innervation revealed in this study appear to have been obtained concomitantly with the acquisition of tracheal chambers in rhinolophids and hipposiderids, improving sound intensity during laryngeal echolocation. In addition, significant protrusion of the sagittal crest of the cricoid cartilage and the separated dorsal cricoarytenoid muscle may contribute to the sophisticated biosonar in this laryngeally echolocating lineage. Furthermore, our bioacoustic data suggested that the mineralization of these cartilages underpins the ontogeny of echolocation pulse generation. The results of the present study provide crucial insights into how the anatomy and development of the hyolaryngeal apparatus shape the acoustic diversity in bats.

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Section: Data Acquisitionmentioning

confidence: 99%

“…ungulates [5], primates [6][7][8][9][10][11][12], manatees [13], rodents [14][15][16][17][18], and bats [19,20]. Structural simplification of the larynx and laryngeal descent results in vocal complexity in humans [12].…”

Section: Introductionmentioning

confidence: 99%

Development of the hyolaryngeal architecture in horseshoe bats: Insights into the evolution of the pulse generation for laryngeal echolocation

Nojiri,

Takechi,

Furutera

et al. 2023

Preprint

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“…The edge‐tone model of whistle production (Riede et al., 2017) predicts that ventral pouch size corresponds to vocal frequencies. This prediction is supported by large ventral pouch sizes in Baiomys (Riede et al., 2020) and Scotinomys (Smith et al., 2021), both species that produce unusually low‐pitched ultrasonic whistles. Our findings herein further support predictions of the edge‐tone hypothesis; young and old mice had similar‐sized ventral pouch volumes and overlapped in their spectral ranges.…”

Section: Discussionmentioning

confidence: 93%

Postnatal remodeling of the laryngeal airway removes body size dependency of spectral features for ultrasonic whistling in laboratory mice

2022

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In many mammals, spectral properties of acoustic signals scale with body size within and among species. In rodents, however, despite drastic changes in body size, fundamental frequency (F0) range of ultrasonic whistles produced for social communication remain relatively uniform from birth to adulthood. Such divergent patterns may be due to a novel sound production mechanism unique to rodents involving an intralaryngeal midline pocket termed the ventral pouch. In this study, we analyzed the postnatal shape and size of the laryngeal airway in CD1 mice over ontogeny to better understand the association between ventral pouch geometry and F0 of ultrasonic whistles. Ventral pouch volume (0.06 ± 0.01 mm3) did not differ between pups and 1‐year‐old adults despite extensive shape‐inducing remodeling of the intralaryngeal musculature and connective tissue. In contrast, ventral pouch volume was 50% less in 2‐year‐old compared to 1‐year‐old mice. Thus, allometry of the laryngeal airway appears to explain spectral overlap between ultrasonic whistles of young, small mice and older, larger mice. The causal association between the reduction in vocal behavior and a seemingly shrinking ventral pouch in geriatric mice remains unclear. Together, these data inform our understanding of the postnatal development and remodeling of the intralaryngeal airway in Mus musculus.

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“…Maximum frequency ( h 2 = 0.88 ± 0.1; ICC = 0.56) is likely a function of the cricothyroid, a muscle controlling the length and tension of vocal folds (Jürgens, 2002; Riede and Brown, 2013). In muroid mice that produce laryngeal whistles, variation in structures like the vocal folds and the ventral pouch are proposed to shape interspecific variation in dominant frequency (Riede et al, 2017; Riede and Pasch, 2020; Smith et al, 2020). At a phylogenetic scale, spectral properties are often constrained by body size, a relationship generally mediated by scaling of vocal structures (Gillooly and Ophir, 2010; Ophir et al, 2010; Ryan and Brenowitz, 1985).…”

Section: Discussionmentioning

confidence: 99%

Genomic heritability of song and condition in wild singing mice

Burkhard

Matz

Phelps

2020

Preprint

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Courtship displays are dramatic examples of complex behaviors that vary within and among species. Evolutionary explanations for this diversity rely upon genetic variation, yet the heritability of complex phenotypes is seldom investigated in the field. Here, we estimate genomic heritability of advertisement song and body condition in a wild population of singing mice. The heritability of song exhibits a systematic pattern, with high heritability for spectral characteristics linked to vocal morphology, intermediate heritability for rhythmic patterns, and lower but significant heritability for measures of motivation, like song length and rate. Physiological measures of condition, like hormonal markers of adiposity, exhibited intermediate heritability. Among singing mice, song rate and body condition have a strong phenotypic correlation; our estimate suggests a comparable genetic correlation that merits further study. Our results illustrate how advances in genomics and quantitative genetics can be integrated in free-living species to address longstanding challenges in behavior and evolution.

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A comparative characterization of laryngeal anatomy in the singing mouse

Abstract: These elaborate behaviors often depend on the modification of, and are ultimately limited by, existing morphology. In Alston's singing mouse (Scotinomys teguina), males attract mates by producing long and loud songs at lower frequencies than most muriod rodents

Cited by 9 publications

References 54 publications

Development of the hyolaryngeal architecture in horseshoe bats: Insights into the evolution of the pulse generation for laryngeal echolocation

Development of the hyolaryngeal architecture in horseshoe bats: Insights into the evolution of the pulse generation for laryngeal echolocation

Postnatal remodeling of the laryngeal airway removes body size dependency of spectral features for ultrasonic whistling in laboratory mice

Genomic heritability of song and condition in wild singing mice

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