The vast majority of existing structures in Croatia, as well as in other European countries, are made of unreinforced masonry. The seemingly ideal building material presents a great challenge for engineers in earthquake-prone zones. Given that structural failure can have significant consequences in terms of loss of human lives as well as financial losses, a comprehensive structural reliability assessment is needed. Old existing masonry structures often do not have design documentation, and even when they do, the initial mechanical properties have almost certainly degraded over time. That is why in situ testing is of great importance, but masonry is also characterized by a large scattering of results. After recent severe earthquakes in Croatia, more than ever, there is a need for a more reliable assessment of existing masonry structures. This paper presents reliability approaches for resistance evaluation of existing masonry in a real case study where basic failure modes of the masonry were observed. First, the data recommended in standards were used, followed by the updated data obtained from the flat-jack test. Finally, the design value method (DVM) and the adjusted partial factor method (APFM) were used. These methods align with Eurocode standards for new structures but are also adaptable for the assessment of existing structures. Differences in the results between the existing and the new version of the standard were observed, as well as an increase in the obtained resistances with an increase in the complexity of the methods used. Also, the influence of in situ testing proved to be an important factor in the analysis.