Improving the driving safety of mountainous highway tunnels has become an urgent problem in China, while the existing literature pays more attention to the safety of urban tunnels. From the perspective of visual load, this paper built a GA-SVR model to analyze the influences of speed, design brightness, measured brightness, and position in the Gaogu long tunnel. The results show the following: firstly, the changes of MTPA in the long mountainous highway tunnels can be divided into five stages, which is different from the three-stage division of urban tunnels; secondly, the influencing degree of factors was varied in different stages: the position factor mattered most in stages 1, 2, 4, and 5, while the design brightness had the greatest impact in stage 3; thirdly, the driver’s psychological pressure was greatest on the entrance and exit section of the tunnel; lastly, the increased length of mountainous highway tunnels and the long-term enclosed driving environment made the psychological load of drivers intensified. Therefore, it was necessary for the mountainous highway tunnels to consider more accurate gradual lighting design in the 200-m sections after the entrance and before the exit, meanwhile enhancing traffic safety management and protections in the middle of the tunnel.