A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay

Abd-Allah, Gamal A.; El-Fayoumi, Refaat Ibrahim; Smith, Micah J.; Heckmann, Richard A.; O’Neill, Kim L.

doi:10.1016/s1383-5718(99)00181-3

Cited by 47 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some interindividual differences were detected, in agreement with other studies (Mitchelmore and Chipman, 1998). Our results also agreed with those of Abd-Allah et al (1999) showing that the comet assay is an effective, simple and rapid method for assessing genetic damage caused by heavy metals in fish. The few reports that have examined fish chromosomal aberrations have been limited to studying the effects of radiation on some cichlid species (Manna and Som, 1982;Som and Manna, 1984).…”

Section: Discussionsupporting

confidence: 92%

“…Sister chromatid exchange (SCE) tests have been applied to various fish species (Kligerman, 1979;Vigfusson et al, 1983) and the clastogenic effects of carcinogenic-mutagenic chemicals on kidney cells of Cyprinius carpio have been described (Al-Sabti, 1986). The comet assay is useful for evaluating genetic alterations and has been used as a rapid method to monitor genotoxicity in bullheads and carp (Monteith and Vanstone, 1995) and to detect the effects of carcinogens, such as aflatoxins, in trout and channel catfish (Abd-Allah et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

et al. 2004

View full text Add to dashboard Cite

The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 µg of Pb 2+ .g -1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the results then compared with three untreated controls kept under lead-free conditions. An additional six treated fish and three controls were sampled for the comet assay after 13 doses (64 days). Exposure to lead significantly increased the frequency of chromosomal aberrations and the frequency of tailed cell nuclei, the latter indicating DNA damage. These results show that H. malabaricus is a useful biological model for screening the clastogenic effects of lead and possibly other xenobiotics. The genetic damage seen here illustrates the need to investigate the potential effects of heavy metals on fish species in South America.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

et al. 2004

View full text Add to dashboard Cite

show abstract

“…The level of DNA in the tail region (tail % DNA) was thought to be the most suitable standard for measuring DNA damage. The results demonstrated higher tail moment in aquatic insects gathered from 2 diverse polluted water streams (Nikla and Elmansoreyah) than the control and (Best and Ross, 1977) likewise got comparable results by examination of change of tail moment revealed significant differences between infected and control fish and the comparative results were gotten by Abd-Allah et al (1999) who utilized the Comet as a basic and quick technique by which DNA damage can be shown as a function of the tail moment. Furthermore, TM has the upside of considering damage communicated as a short tail with a high division of DNA or a long tail with a low division of DNA (White and Rasmussen, 1998).…”

Section: The Statistical Analysis Of Dna Damagementioning

confidence: 88%

Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Afify

2017

jmr

View full text Add to dashboard Cite

River Nile represents the most important source of freshwater in Egypt. There are several factors lead to the water pollution in the River Nile System (main stream Nile, drains and canals). The water quality of River Nile was good despite high organic loads discharged from some of the drains and industrial activities. These were harmful both to human and stream ecosystem health. A biological approach to monitoring water quality incorporates use of stream organisms themselves as a basis for pollution detection. Fish and aquatic insects considered as bio-indicators of pollutant effects and help to investigate possible environmental problems. One of the recent biochemical techniques for detecting DNA damage as a result of DNA single strand breaks, alkali-labile sites, and cross-linking is the alkaline (pH>13) single cell gel SCG assay. In this study the comet assay measure the DNA damage in various stages of the mosquito Culex pipiens collected from two different polluted water streams (Nikla and Elmansoreyh). The DNA damage measured in 3 rd , 4 th larval instars, pupa, male and female adults. It is clear from the results obtained in this study that the genotoxicity of water pollution of two different polluted water streams (Nikla and Elmansoreyh) in C. pipiens was high in larval and pupal stages and this study affirmed the appropriateness of the comet assay as a sensitive tool for environmental monitoring. Additionally, it can be proposed that C. pipiens is a strong aquatic bioindicator of genotoxicity.

show abstract

“…Also known as a Comet assay, this is a sensitive technique used to detect DNA damage in biological systems which serves to expose alkali labile sites and strand breaks (Abd-Allah et al 1999). Staining with ethidium bromide reveals increased extension of the Table 4 Circulating total leukocyte, granulocyte and lymphocyte numbers (1 £ 10 9 l ¡1 ) in peripheral blood before (PRE), immediately following (IPE) and 3 h following (3PE) 2.…”

Section: Discussionmentioning

confidence: 99%

Prolonged exercise does not cause lymphocyte DNA damage or increased apoptosis in well-trained endurance athletes

et al. 2006

View full text Add to dashboard Cite

Recent research has demonstrated that lymphocyte apoptosis sensitivity appears to be related to training status and exercise intensity. This work investigated the effect of prolonged, submaximal treadmill running on percentage (%) apoptosis, % necrosis and DNA strand breaks in lymphocytes and related these to changes in total lymphocyte and blood cortisol concentrations in well-trained runners. Venous blood samples (n = 14) were taken immediately before (PRE), immediately after (IPE) and 3 h after (3PE) 2.5 h of treadmill running at 75% of VO2 max from eight well-trained male endurance athletes (age 34.2 +/- 2.44 years) and analysed for cellular content and serum cortisol concentrations. Lymphocytes were isolated from whole blood and % apoptotic and necrotic cell were detected by flow cytometry using Annexin V-FITC and propidium iodide uptake. DNA strand breaks were measured by single-cell gel electrophoresis. Despite a significant (P < 0.001) exercise-induced increase in mean serum cortisol concentrations and reduction in lymphocyte counts, the mean % Annexin-V positive cells (13.3 +/- 6.78 in PRE, 11.3 +/- 5.51 in IPE and 12.8 +/- 6.75 in 3PE samples) were not significantly different at the three time-points (P > 0.05). Mean DNA strand breaks in the lymphocytes also did not change significantly (P > 0.05) rising from 25.7 +/- 2.16 to 26.9 +/- 1.89 and 27.1 +/- 1.38 microm in IPE and 3PE samples, respectively. The exercise-induced changes in total blood lymphocyte counts and cortisol concentrations did not result in a significant change in % apoptotic lymphocytes or DNA strand breaks in the endurance-trained athletes during this prolonged, submaximal exercise.

show abstract

A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay

Cited by 47 publications

References 32 publications

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

Potential Role of Mosquito Larvae <i>Culex Pipiens</i> as a Biological Indicator of Environmental Water Pollution in Egypt

Prolonged exercise does not cause lymphocyte DNA damage or increased apoptosis in well-trained endurance athletes

Contact Info

Product

Resources

About