A comparative evaluation of biological activities and bioactive compounds of the seagrasses <i>Zostera marina</i> and <i>Zostera noltei</i> from southern Portugal

Custódio, Luísa; Laukaityte, Simona; Engelen, Aschwin H.; Rodrigues, Maria João; Pereira, Hugo; Vizetto‐Duarte, Catarina; Barreira, Luísa; Rodríguez, Hortensia; Alberício, Fernando; Varela, João

doi:10.1080/14786419.2015.1040791

Cited by 16 publications

(11 citation statements)

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“…marina , e.g. rosmarinic acid (RA), zosteric acid (ZA), and sulfated flavonoids are known to act as antimicrobial or antifouling agents 17–21 . However, in order to be involved in chemical signaling and fight epibiosis, these allelochemicals would require to be produced intracellularly and then to be released into the first site of contact with the microfoulers, i.e.…”

Section: Introductionmentioning

confidence: 99%

Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Papazian

Parrot

Burýšková

et al. 2019

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Plants rely on both mechanical and chemical defence mechanisms to protect their surfaces against microorganisms. The recently completed genome of the eelgrass Zostera marina , a marine angiosperm with fundamental importance for coastal ecosystems, showed that its re-adaptation from land to the sea has led to the loss of essential genes (for chemical communication and defence) and structural features (stomata and thick cuticle) that are typical of terrestrial plants. This study was designed to understand the molecular nature of surface protection and fouling-control strategy of eelgrass against marine epiphytic yeasts. Different surface extraction methods and comparative metabolomics by tandem mass spectrometry (LC-MS/MS) were used for targeted and untargeted identification of the metabolite profiles of the leaf surface and the whole tissue extracts. Desorption electrospray ionization-imaging mass spectrometry (DESI-IMS) coupled with traditional bioassays revealed, for the first time, the unique spatial distribution of the eelgrass surface-associated phenolics and fatty acids, as well as their differential bioactivity against the growth and settlement of epiphytic yeasts. This study provides insights into the complex chemical defence system of the eelgrass leaf surface. It suggests that surface-associated metabolites modulate biotic interactions and provide chemical defence and structural protection to eelgrass in its marine environment.

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Section: Introductionmentioning

confidence: 99%

Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Papazian

Parrot

Burýšková

et al. 2019

Sci Rep

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“…However, consistent with previous results for Z . noltei 30, 60, 61 , these FA relative content correspond to a very small proportion of total FA (≤5%, altogether). Moreover, palmitoleic (16:1 n -7) and eicosapentaenoic acid (20:5 n -3) are frequently used as diatom markers, while docosahexaenoic acid (22:6 n -3) is a well-established marker for flagellates 62 .…”

Section: Discussionmentioning

confidence: 92%

“…noltei at different geographical regions such as Ria de Aveiro 60 and Ria Formosa 61 , in Portugal and Marennes-Oléron Bay, in the French Atlantic coast 30 . Also, the high PUFA relative content in Z .…”

Section: Discussionmentioning

confidence: 99%

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Effect of spatio-temporal shifts in salinity combined with other environmental variables on the ecological processes provided by Zostera noltei meadows

Sousa

Calado

Cleary

et al. 2017

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The present study aims to assess the plastic response of Zostera noltei meadows traits under spatio-temporal shifts in salinity combined with sediment environmental variables (temperature; pH; loss-on-ignition (LOI); carbon (C) and nitrogen (N) pools (top 5 cm)). Z. noltei biomass, C and N pools, leaf photosynthetic performance and esterified fatty acid (FA) profile were assessed within a temperate coastal lagoon during winter and late spring, along sites spatially distributed. None of the surveyed traits for Z. noltei displayed a clear spatial trend. Z. noltei proved to be euryhaline, whose biology was only slightly affected within this salinity range, in each season (14–39 in winter; 33–41 in late spring). Seasonal differences in salinity and environmental parameters explain the differences recorded in Z. noltei traits (aboveground biomass, N and C pools; photosynthetic performance). Spatio-temporal salinity shifts did not significantly affect the pool of FA present in Z. noltei. Overall, within the salinity range surveyed, the ecological processes studied and regulating Z. noltei meadows do not appear to be at risk. This work reinforces the plasticity of Z. noltei to salinity shifts within the studied range, with this finding being particularly relevant in the context of extreme weather events (e.g., winter freshwater floods, summer droughts).

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“…tandem MS n can provide detailed structural information [47]. Furthermore, RP-HPLC has been used to isolate and purify a natural bioactive compound named rosmarinic acid from Z. marina and phenyl thioketone from Cymodocea serrulata, both had high antioxidant, anticarcinogenic and anti-microbial properties [52,53]. In recent years, ultra-high performance liquid chromatography (UHPLC) is the method of choice for metabolomics research rather than RP-HPLC, because it provides greater resolution, peak capacity and lower detection limits with the use of small particle size columns (1.7 μm).…”

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confidence: 99%

Metabolomics: an emerging frontier of systems biology in marine macrophytes

et al. 2016

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Metabolomics is a rapidly emerging discipline within functional genomics which is increasingly being applied to 19 understand biochemical phenotypes across a range of biological systems. Metabolomics measures all (or a 20 subset) metabolites in a cell at a specific time point, reflecting a snapshot of all the regulatory events responding 21 to the external environmental conditions. Although metabolomics and system biology approaches have been ap-22 plied to the study of terrestrial plants, few marine macrophytes have been examined using these novel technol-23 ogies. Marine macrophytes (including seaweeds and seagrasses) are marine ecosystem engineers delivering a 24 range of ecologically and economically valuable biological services; however they are under threat from a wide 25 range of anthropogenic stressors, climate variation, invasive species and pathogens. Investigating metabolomic 26 regulation in these organisms is crucial to understand their acclimation, adaptation and defence responses to 27 environmental challenges. This review describes the current analytical tools available to study metabolomics 28 in marine macrophytes, along with their limitations for both targeted and non-targeted workflows. To illustrate 29 recent advances in system biology studies in marine macrophytes, we describe how metabolites are used in 30 chemical defence to deter a broad range of invasive species and pathogens, as well as metabolomic 31 reprogramming leading to acclimation or adaptive strategies to environmental and anthropogenic stresses. 32 Where possible, the mechanistic processes associated with primary and secondary plant metabolism governing 33 cellular homeostasis under extreme environments are discussed. Functional integration of metabolomics with 34 the allied "omics" disciplines of transcriptomics and proteomics, as well as the emerging discipline of "fluxomics" 35 are discussed in the context of developing biological system networks, the identification of unknown gene/pro-36 tein functions and the analysis of metabolic pathways in marine plants exposed to stress. Finally, we provide a 37 comprehensive overview of an in silico plant metabolome database that can be utilized to advance our knowl-38 edge from a system biology approach to marine macrophytes.

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A comparative evaluation of biological activities and bioactive compounds of the seagrasses Zostera marina and Zostera noltei from southern Portugal

Cited by 16 publications

References 11 publications

Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Effect of spatio-temporal shifts in salinity combined with other environmental variables on the ecological processes provided by Zostera noltei meadows

Metabolomics: an emerging frontier of systems biology in marine macrophytes

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