A comparative evaluation of poly-l-lysine-palmitic acid and Lipofectamine ™ 2000 for plasmid delivery to bone marrow stromal cells

Clements, Başak Açan; Incani, Vanessa; Kucharski, Cezary; Lavasanifar, Afsaneh; Ritchie, Bruce; Uludağ, Hasan

doi:10.1016/j.biomaterials.2007.07.023

Cited by 88 publications

(72 citation statements)

References 47 publications

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“…MSCs were infected with the 50 multiplicity of infection (MOI; particle forming units per cell) of adenovirus for 48 hours. We choose the AdGFP as control virus and had consistent results with Clements et al in transfection efficiency and long-term assessment for hBMSCs [27].…”

Section: Adenoviral Transfectionsmentioning

confidence: 84%

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

et al. 2009

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The senescence of human mesenchymal stem cells (hMSCs) causes disruption of tissue and organ maintenance, and is thus an obstacle to stem cell-based therapies for disease. Although some researchers have studied changes in the characteristics of hMSCs (decreases in differentiation ability and self-renewal), comparing young and old ages, the mechanisms of stem cell senescence have not yet been defined. In this study, we developed a growth curve for human bone marrow derived MSCs (hBMSCs) which changes into a hyperbolic state after passage number 7. Senescence associated b-galactosidase (SA b-gal) staining of hBMSCs showed 10% in passage 9 and 45% in passage 11. We detected an increase in endogenous superoxide levels during senescence that correlated with senescence markers (SA b-gal, hyperbolic growth curve). Interestingly, even though endogenous superoxide increased in a replicative senescence model, the expression of APE1/Ref-1, which is sensitive to intracellular redox state, decreased. These effects were confirmed in a stressinduced senescence model by exogenous treatment with H 2 O 2 . This change is related to the p53 activity that negatively regulates APE1/Ref-1. p21 expression levels, which represent p53 activity, were transiently increased in passage 9, meaning that they correlated with the expression of APE1/Ref-1. Overexpression of APE1/Ref-1 suppressed superoxide production and decreased SA b-gal in hBMSCs. In conclusion, intracellular superoxide accumulation appears to be the main cause of the senescence of hBMSCs, and overexpression of APE1/Ref-1 can rescue cells from the senescence phenotype. Maintaining characteristics of hBMSCs by regulating intracellular reactive oxygen species production can contribute to tissue regeneration and to improved cell therapy.

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Section: Adenoviral Transfectionsmentioning

confidence: 84%

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

et al. 2009

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“…In the context of alginate, we did not observe a clear additive effect from the use of liposomal transfection agent, even though literature describes this as an even more successful transfection method than the use of calcium phosphate precipitation 186 . Normally the positively charged liposome complexes make it easier for DNA liposome complexes to access the negatively charged cell membrane 135 . The sugarchains of alginate however, might neutralize the effect of liposome complexation on the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

“…The DNA uptake and thus transfection efficiencies were enhanced by 10-fold, up to 22 %, making it comparable to liposome-based transfection efficiencies, which were used as a control 134 . The combination of both increased transfection efficiencies even more 135 . Other examples of synthetic polymer-based delivery agents are the biodegradable polylactic acid (PLA), poly(lactide-co-glycolide) (PLG) and poly(lactic-co-glycolic acid) (PLGA) 122 136 .…”

Section: Synthetic Polymer-based Transfectionsmentioning

confidence: 96%

Non-viral gene therapy for bone tissue engineering

Wegman

2013

Biotechnology and Genetic Engineering Reviews

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“…Generally, viral (e.g., adenoviruses, lentiviruses and retroviruses) and nonviral vectors (e.g., lipids and polymers) can be used for cellular transfection and/or nucleofection, thus offering durable gene expression within stem cells [93][94][95][96]. Nonviral carriers have a number of advantages over viral vectors, since they exhibit low-risk immunogenicity and insertional mutagenesis, controllable toxicity, and excellent gene-carrying capacity [95].…”

Section: A U T H O R P R O O Fmentioning

confidence: 99%

“…Many efforts for the improvement of nonviral vectors are focused on cationic polymers that interact with negatively charged DNA or RNAi. Polymers, including poly(l-lysine)-palmitic acid, poly(l-lysine) and polyethylenimine, condense the genetic material into particles of 200-300 nm in diameter, protect them from enzymes and facilitate cellular entrance [94,97]. These complexes of polymers and genetic material (called 'polyplexes') have a transfection efficiency that is equivalent to adenoviral vectors [97].…”

Section: A U T H O R P R O O Fmentioning

confidence: 99%

Nanodentistry: Combining Nanostructured Materials and Stem Cells for Dental Tissue Regeneration

2012

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Regenerative dentistry represents an attractive multidisciplinary therapeutic approach that complements traditional restorative/surgery techniques and benefits from recent advances in stem cell biology, molecular biology, genomics and proteomics. Materials science is important in such advances to move regenerative dentistry from the laboratory to the clinic. The design of novel nanostructured materials, such as biomimetic matrices and scaffolds for controlling cell fate and differentiation, and nanoparticles for diagnostics, imaging and targeted treatment, is needed. The combination of nanotechnology, which allows the creation of sophisticated materials with exquisite fine structural detail, and stem cell biology turns out to be increasingly useful in regenerative medicine. The administration to patients of dynamic biological agents comprising stem cells, bioactive scaffolds and/or nanoparticles will certainly increase the regenerative impact of dental pathological tissues. This overview briefly describes some of the actual benefits and future possibilities of nanomaterials in the emerging field of stem cell-based regenerative dentistry.

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A comparative evaluation of poly-l-lysine-palmitic acid and Lipofectamine ™ 2000 for plasmid delivery to bone marrow stromal cells

Cited by 88 publications

References 47 publications

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

Downregulation of APE1/Ref-1 Is Involved in the Senescence of Mesenchymal Stem Cells

Non-viral gene therapy for bone tissue engineering

Nanodentistry: Combining Nanostructured Materials and Stem Cells for Dental Tissue Regeneration

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