A Comparative Immunohistochemical Analysis of Langerhans Cells in Oral Mucosa, Oral Lichen Planus and Oral Squamous Cell Carcinoma

Maloth, Aruna Kumari; Dorankula, Shyam Prasad Reddy; Pasupula, Ajay Prakash; Thokala, Madhusudan Rao; Muddana, Keerthi; Ramavath, Ravinder

doi:10.7860/jcdr/2015/14170.6235

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…Quantification of positive cells was performed by a single pathologist using a light microscope (Nikon, Tokyo, Japan) with a 40× eyepiece in five sequential fields randomly selected . In the OSCC group, cells were counted in the epithelial nests and surrounding stroma.…”

Section: Methodsmentioning

confidence: 99%

“…The antibody dilutions, clone, AR used, stained cells and positive controls are given in Table 1 Quantification of positive cells was performed by a single pathologist using a light microscope (Nikon, Tokyo, Japan) with a 409 eyepiece in five sequential fields randomly selected. 17 In the OSCC group, cells were counted in the epithelial nests and surrounding stroma. In the OED group, cells were counted in the epithelial layer and in the lamina propria.…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Immunosurveillance profile of oral squamous cell carcinoma and oral epithelial dysplasia through dendritic and T‐cell analysis

Pellicioli

Bingle

Farthing

et al. 2017

J Oral Pathology Medicine

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The number of mature dendritic cells (DC) was decreased in OSCC compared with OED lesions suggesting that either these cells might have migrated to lymph nodes to present the tumour antigens and activate the immune system or cytokines secreted by the tumour microenvironment are inhibiting the adequate maturation of DLC. The numbers of pDC were significantly increased in the OSCC group compared with the OED group. This suggests they may play an important role in the defence against tumours although it is not clear whether this is promoting or inhibiting malignant progression.

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Section: Methodsmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Immunosurveillance profile of oral squamous cell carcinoma and oral epithelial dysplasia through dendritic and T‐cell analysis

Pellicioli

Bingle

Farthing

et al. 2017

J Oral Pathology Medicine

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“…Это обстоятельство, по-видимому, можно трактовать как косвенный аргумент в пользу предположения о том, что для полноценного функционирования ДК должны взаимодействовать с высокодифференцированными клетками плоского эпителия. В определенной степени предположение подтверждается данными исследований, в которых показано, что наибольшее количество КЛ обнаруживалось в высокодифференцированных опухолях, наименьшее -в низкодифференцированных новообразованиях [10].…”

Section: Discussionunclassified

Characteristics of inflammatory reactions in the tumor microenvironment of head and neck squamous cell carcinoma

et al. 2019

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Objective: to analyze the association between inflammatory cells with morphological heterogeneity and lymphogenous metastasis of head and neck squamous cell carcinoma.Material and Methods. Five types of tumor structures, the level of inflammatory infiltration and the counts of CD4+ and CD8+ t-lymphocytes, B-lymphocytes and macrophages in the stromal infiltrate and Langerhans cells in tumor structures were assessed in 44 patients.Results. The presence of Langerhans cells in type 1 structures was correlated with the presence of CD3+ and CD4+ t-cells in the inflammatory infiltrate. There was a decrease in the inflammatory reaction and the number of CD3+ T cells in patients with metastatic lymph nodes compared with patients with intact lymph nodes. In patients with lymph node metastasis, the proportion of CD4+ and / or CD8+ and / or CD20+ lymphocytes in the infiltrate was higher in cases with well-differentiated tumor than in cases with poorly-differentiated tumor.Conclusion. The results obtained showed the decrease in the level of inflammatory response, including t-cell response, as well as the relationship between the presence of Langerhans cells and stromal inflammatory response in patients with lymph node metastasis.

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“…This study has shown how LCs increase significantly from a mild or moderate to a severe inflammatory state. A study by Maloth et al (2015) compared LCs data in oral Lichen planus (OLP) and oral squamous cell carcinoma (OSCC), by anti-S-100 antibodies. The study aimed to demonstrate the function of LCs in local immune response.…”

Section: S-100mentioning

confidence: 99%

“…Histomorphometric quantification was made only on S-100 positive cells, excluding those of the basal layer, identified as melanocytes. The positivity of the reaction has shown that LCs play crucial roles in various oral pathological conditions ( Maloth et al, 2015 ). Also, in a study on the histiocytosis of LCs in an adult with diabetes insipidus conducted by Allen et al (2019) , the anti-S-100 antibody was used to highlight LCs in the tissue samples studied.…”

Section: S-100mentioning

confidence: 99%

Marking vertebrates langerhans cells, from fish to mammals

et al. 2020

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Langerhans cells (LCs) are specialized dendritic cells (DCs) that play a defense role in recognizing foreign antigens, in tissue where antigenic exposures occur, as in the skin and mucous membranes. LCs are able to continuously move within the tissues thanks to dendritic contraction and distension performing their surveillance and/or phagocytosis role. These cells are characterized by the presence of Birbeck granules in their cytoplasm, involved in endocytosis. LCs have been characterized in several classes of vertebrates, from fish to mammals using different histological and molecular techniques. The aim of the present review is to define the state of art and the need of information about immunohistochemical markers of LCs in different classes of vertebrates. The most used immunohistochemical (IHC) markers are Langerin/CD207, CD1a, S-100 and TLR. These IHC markers are described in relation to their finding in different vertebrate classes with phylogenetical considerations. Among the four markers, Langerin/CD207 and TLR have the widest spectrum of cross reactivity in LCs.

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A Comparative Immunohistochemical Analysis of Langerhans Cells in Oral Mucosa, Oral Lichen Planus and Oral Squamous Cell Carcinoma

Abstract: A better understanding and clarity of LCs is pivotal for designing novel or improved therapeutic approaches that will allow proper functioning of LC's in patients with OLP and OSCC, thus significantly reducing the morbidity of OLP and OSCC patients.

Cited by 5 publications

References 12 publications

Immunosurveillance profile of oral squamous cell carcinoma and oral epithelial dysplasia through dendritic and T‐cell analysis

Immunosurveillance profile of oral squamous cell carcinoma and oral epithelial dysplasia through dendritic and T‐cell analysis

Characteristics of inflammatory reactions in the tumor microenvironment of head and neck squamous cell carcinoma

Marking vertebrates langerhans cells, from fish to mammals

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