A Comparative Immunological Study of Pituitary Growth Hormone From Various Species

Hayáshida, T.; Li, Choh Hao

doi:10.1210/endo-65-6-944

Cited by 57 publications

(11 citation statements)

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“…The antigenic specificity of HGH has been studied and extensively described by previous workers (7,9,24,25). Our observations with radio-immunoprecipitation confirm the information obtained with more orthodox immunologic procedures showing that antisera induced in rabbits by HGH reacts only with other primate growth hormones and not with growth hormones of other species.…”

Section: Discussionsupporting

confidence: 87%

Studies on Human Growth Hormone. I. A Radio-Immunoassay for Human Growth Hormone*

Utiger¹,

Parker²,

Daughaday³

1962

J. Clin. Invest.

181

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Section: Discussionsupporting

confidence: 87%

Studies on Human Growth Hormone. I. A Radio-Immunoassay for Human Growth Hormone*

Utiger¹,

Parker²,

Daughaday³

1962

J. Clin. Invest.

181

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“…The similarities of ovine and bovine GH and of porcine and cetacean GH in elution volumes and in the electrophoretic mobilities of the likely active components on starch gel are matched by similarities in other physico-chemical and immunological properties of these pairs (Papkoff & Li, 1958a;Hayashida & Li, 1959;Papkoff, Li & Liu, 1962;.…”

Section: Discussionmentioning

confidence: 85%

The Chromatography on Deae-Cellulose of Pituitary Extracts From Several Species

Wallace¹,

Ferguson²

1964

Journal of Endocrinology

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The distribution, potency and yield of growth hormone (GH), prolactin, thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) activities and the electrophoretic behaviour on starch gel have been studied in fractions obtained by chromatography on DEAE-cellulose of pituitary extracts from man, sheep, ox, pig and whale.In none of the species examined was the distribution of hormonal activities identical. GH, TSH and FSH in the sheep and ox, and GH and LH in the pig and whale, appeared in corresponding fractions. The prolactin activities from the five species were found in closely similar elution volumes. The hormonal activities of the human extract occurred over a much smaller range of distribution volumes than those of the other species. The GH fractions from all five species and the prolactins from sheep and ox were relatively potent preparations.Starch gel electrophoresis showed a number of components in all fractions. The distribution of components in corresponding fractions was different for each species, although some components had similar mobilities.These results add to the growing evidence of the species-specific nature of pituitary hormones and to the information available for evolving comprehensive fractionation systems for each species.

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“…Cross neutralization of TSH activity was demonstrated between the anti-bovine TSH antisera and TSH from mouse pituitary tumor and human pituitary sources. Thus, antisera to TSH would seem not to be as highly species specific as antisera to growth hormone (17), and this is in good correspondence with the known effectiveness of TSH from bovine and other sources in heterologous species.…”

Section: Discussionmentioning

confidence: 87%

Immunologic and Biologic Characterization of Antisera to Beef Thyrotropin Preparations*

Werner¹,

Seegal²,

Osserman³

1961

J. Clin. Invest.

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(CY 2332) and National Heart Institute Grant H 3923. METHODSThyrotropic hormone preparations. Three bovine thyroid-stimulating hormone (TSH) preparations were used in these studies. One, in clinical use under the trade name Thytropar, contained approximately 1 international unit (IU) per mg ("Armour"). The two other preparations contained greater activity ranging from 5 to 25 IU per mg, and were provided through the kindness of Dr. Peter G. Condliffe and Dr. Rohert W. Bates ("Condliffe"), and Drs. John L. Pierce and M. E. Carsten ("Pierce"). Both of these latter preparations were concentrated on anion diethylaminoethyl (DEAE) and cation (carboxymethyl cellulose) exchange resins (6, 7) with some variations in methodology between laboratories. A mouse pituitary tumor thyrotropin preparation was made available by Dr. Condliffe and Dr. Bates (8) and a preparation of human thyrotropin by Dr. John Bakke and Dr. Peter Heidemann.Pituitary hormone preparations other than 'ISH. A number of pituitary hormone preparations other than TSH were tested for cross reaction with the anti-TSH sera. The sources of these preparations are listed in Table II. All preparations termed "Wilhelmi" were provided by both Dr. Alfred E. Wilhelmi and Dr. Stanley Ellis. The bovine growth hormone and prolactin preparations were obtained as a gift of the Endocrine Study Section, National Institutes of Health.Preparation of antisera. The antisera used for the precipitin and in vivro neutralization studies were developed in rabbits. Fifteen animals were immunized: 9 with Armour TSH, and 3 animals each with the Condliffe and Pierce TSH preparations, respectively. Three IU of thyrotropin was incorporated in 1.0 ml of Freund adjuvant and injected intracutaneously in multiple sites (9). The injections were repeated 2 or 3 times, at weekly or biweekly intervals. A total of 9 or 12 U was given to each rabbit. Ten days after the final injection, the animals were exsanguinated under sterile conditions and the sera were separated and stored without preservative at 40 C. After the initial precipitin studies, absorption of the antisera to TSH with bovine serum was routinely performed. This was accomplished in the following manner: 0.05 ml of bovine serum was added to each 1.0 ml of antiserum and allowed to stand 5 to 7 days at 40 C. The precipitate was then removed by centrifugation, and another 0.05 ml of bovine serum added to the supernatant. This procedure was repeated until there was no further 92

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A Comparative Immunological Study of Pituitary Growth Hormone From Various Species

Cited by 57 publications

References 0 publications

Studies on Human Growth Hormone. I. A Radio-Immunoassay for Human Growth Hormone*

Studies on Human Growth Hormone. I. A Radio-Immunoassay for Human Growth Hormone*

The Chromatography on Deae-Cellulose of Pituitary Extracts From Several Species

Immunologic and Biologic Characterization of Antisera to Beef Thyrotropin Preparations*

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