A comparative in vitro and in vivo study of porcine‐ and bovine‐derived non‐cross‐linked collagen membranes

Zhu, Mengdi; Duan, Beibei; Hou, Kegui; Mao, Lisha; Wang, Xuejiu

doi:10.1002/jbm.b.35174

Cited by 10 publications

(6 citation statements)

References 59 publications

(106 reference statements)

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“…A slowest resorption rate was observed with the pullulan/dextran-derived membranes compared to the commercial collagen membrane. This finding was also supported by previous studies reporting the tendency of collagen membranes to quickly resorb [43,[46][47][48]. A possible explanation is the progressive cellular infiltration observed inside the Bio-Gide ® membrane, whereas no cell infiltration was observed inside Mb and Mb + HA.…”

Section: Discussionsupporting

confidence: 89%

Development of Novel Polysaccharide Membranes for Guided Bone Regeneration: In Vitro and In Vivo Evaluations

Ahmed Omar,

Roque,

Galvez

et al. 2023

Bioengineering

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Introduction: Guided bone regeneration (GBR) procedures require selecting suitable membranes for oral surgery. Pullulan and/or dextran-based polysaccharide materials have shown encouraging results in bone regeneration as bone substitutes but have not been used to produce barrier membranes. The present study aimed to develop and characterize pullulan/dextran-derived membranes for GBR. Materials and methods: Two pullulan/dextran-based membranes, containing or not hydroxyapatite (HA) particles, were developed. In vitro, cytotoxicity evaluation was performed using human bone marrow mesenchymal stem cells (hBMSCs). Biocompatibility was assessed on rats in a subcutaneous model for up to 16 weeks. In vivo, rat femoral defects were created on 36 rats to compare the two pullulan/dextran-based membranes with a commercial collagen membrane (Bio-Gide®). Bone repair was assessed radiologically and histologically. Results: Both polysaccharide membranes demonstrated cytocompatibility and biocompatibility. Micro-computed tomography (micro-CT) analyses at two weeks revealed that the HA-containing membrane promoted a significant increase in bone formation compared to Bio-Gide®. At one month, similar effects were observed among the three membranes in terms of bone regeneration. Conclusion: The developed pullulan/dextran-based membranes evidenced biocompatibility without interfering with bone regeneration and maturation. The HA-containing membrane, which facilitated early bone regeneration and offered adequate mechanical support, showed promising potential for GBR procedures.

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Section: Discussionsupporting

confidence: 89%

Development of Novel Polysaccharide Membranes for Guided Bone Regeneration: In Vitro and In Vivo Evaluations

Ahmed Omar,

Roque,

Galvez

et al. 2023

Bioengineering

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“…In an in vitro test, Zhu et al observed a correlation between larger pore size and increased liquid absorption capacity and swelling behavior [ 26 ]. This could indicate a superior ability to absorb blood and tissue fluid, and thus, endogenous and added exogenous growth factors.…”

Section: Discussionmentioning

confidence: 99%

“…Swelling is another crucial parameter. A clinical correlation is believed to exist between a high swelling ratio and rapid resorption rate [ 26 ]. After implantation and suturing of the flap, the collagen materials are compressed to varying extents.…”

Section: Introductionmentioning

confidence: 99%

Properties of three collagen scaffolds in comparison with native connective tissue: an in-vitro study

Solderer,

Widmer,

Gubler

et al. 2023

Int J Implant Dent

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Purpose To evaluate collagen scaffolds (CS) in terms of their in vitro resorption behavior, surface structure, swelling behavior, and mechanical properties in physiologically simulated environments, compared with porcine native connective tissue. Materials and methods Three test materials—one porcine collagen matrix (p-CM), two acellular dermal matrices (porcine = p-ADM, allogenic = a-ADM)—and porcine native connective tissue (p-CTG) as a control material were examined for resorption in four solutions using a high-precision scale. The solutions were artificial saliva (AS) and simulated body fluid (SBF), both with and without collagenase (0.5 U/ml at 37 °C). In addition, the surface structures of CS were analyzed using a scanning electron microscope (SEM) before and after exposure to AS or SBF. The swelling behavior of CS was evaluated by measuring volume change and liquid absorption capacity in phosphate-buffered saline (PBS). Finally, the mechanical properties of CS and p-CTG were investigated using cyclic compression testing in PBS. Results Solutions containing collagenase demonstrated high resorption rates with significant differences (p < 0.04) between the tested materials after 4 h, 8 h and 24 h, ranging from 54.1 to 100% after 24 h. SEM images revealed cross-linked collagen structures in all untreated specimens. Unlike a-ADM, the scaffolds of p-CM and p-ADM displayed a flake-like structure. The swelling ratio and fluid absorption capacity per area ranged from 13.4 to 25.5% among the test materials and showed following pattern: p-CM > a-ADM > p-ADM. P-CM exhibited higher elastic properties than p-ADM, whereas a-ADM, like p-CTG, were barely compressible and lost structural integrity under increasing pressure. Conclusions and clinical implications Collagen scaffolds vary significantly in their physical properties, such as resorption and swelling behavior and elastic properties, depending on their microstructure and composition. When clinically applied, these differences should be taken into consideration to achieve the desired outcomes. Graphical Abstract

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“…Collagen stabilized scaffolds (coll-UV) and composite scaffolds (coll-PHNQ) demonstrated lower water uptake. This water uptake is indicative of scaffold responsiveness to aqueous environments, such as blood, with materials exhibiting high water holding capacity correlating positively with improved angiogenesis, beneficial for tissue regeneration 37 . Moreover, marine collagen, naturally enriched in glycosaminoglycans (GAGs), contributes to this water retention capability 38 .…”

Section: Amino Acidsmentioning

confidence: 99%

Seas of Renewal: Turning Sea Urchin Waste into Polyhydroxynaphtoquinone-Collagen Biomaterials for Skin Regeneration

Martinelli,

Marzorati,

Roncoroni

et al. 2024

Preprint

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Chronic wounds and skin ulcers pose significant challenges to healthcare systems globally, necessitating innovative approaches to expedite healing processes. Biomaterial-based therapies have emerged as promising solutions for tissue regeneration. This study focuses on valorization of sea urchin waste towards the development and characterization of collagen-based scaffolds added with polyhydroxynaphthoquinones (PHNQs) antioxidants, successfully incorporated into biomaterials at optimal ratio, enhancing scaffold stability and integrity. Mechanical properties, water uptake, and degradation kinetics of the composite scaffolds were evaluated and compared with controls. Results indicate that composite scaffolds exhibit superior mechanical stability and slower degradation rates, attributed to strong interactions between collagen and PHNQs. This aspect was explored also through in silico investigations by means of Tight Binding Molecular Dynamics methods. It has been found that a covalent bond forms between the selected collagen representative and one PHNQ. Furthermore, the antioxidant activity of PHNQs was retained in the composite scaffolds, providing additional therapeutic benefits. Overall, these findings underscore the potential of collagen-based composite scaffolds as advanced wound healing therapeutics, combining regenerative properties with antioxidant effects for improved clinical outcomes.

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A comparative in vitro and in vivo study of porcine‐ and bovine‐derived non‐cross‐linked collagen membranes

Cited by 10 publications

References 59 publications

Development of Novel Polysaccharide Membranes for Guided Bone Regeneration: In Vitro and In Vivo Evaluations

Development of Novel Polysaccharide Membranes for Guided Bone Regeneration: In Vitro and In Vivo Evaluations

Properties of three collagen scaffolds in comparison with native connective tissue: an in-vitro study

Seas of Renewal: Turning Sea Urchin Waste into Polyhydroxynaphtoquinone-Collagen Biomaterials for Skin Regeneration

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