A Comparative In Vitro Study of Two Denture Cleaning Techniques as an Effective Strategy for Inhibiting Candida albicans Biofilms on Denture Surfaces and Reducing Inflammation

Abstract

The data indicate that maintaining good oral health for denture wearers requires daily use of a denture cleanser rather than an alternating regimen. The inability of the denture cleanser to sterilize during intermittent treatments demonstrates the difficulty in controlling established biofilm. Moreover, the presence of mature biofilm may result in high levels of inflammation, but this can be controlled through denture cleansing.

Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity

“…These studies do not confirm the biofilm forming capacity of the assayed isolates, whereas our study used known HBFs, which could explain why our isolates were more resistant to treatment, a phenomenon reported elsewhere with these phenotypes (11). Moreover, previous studies have shown that C. albicans is not completely eradicated from the denture, even when using various denture cleansing treatments (29). Our collection of SEM images supports this as they show that C. albicans cells remain on the denture acrylic irrespective of treatment.…”

“…Therefore, qPCR was used instead. Biofilms were sonicated at 35 kHz for 10 min in 1 ml PBS to remove the biomass (29). Samples were centrifuged for 10 min at 10000 x g, the supernatant discarded and DNA extracted, as previously described.…”

Dentures are a Reservoir for Respiratory Pathogens

“…We have shown a high prevalence of putative respiratory pathogens on the dentures of ambulatory adults, a finding that could explain the source of infection in some cases of AP. Adoption of routine oral hygiene practices including mechanical cleaning, and the use of antiseptic cleansing agents could help to reduce the risk of respiratory infection among the elderly population . Further detailed microbiological investigations using next‐generation sequencing will further aid our understanding of the risks associated with ono‐compliance of denture cleanser regimens.…”

Efficacy of denture cleaners on the surface roughness and <i>Candida albicans</i> adherence of sealant agent coupled denture base materials

“…Dentists usually advise patients the overnight immersion, to remain their oral tissues in relax and to clean the dentures during sleeping period 17) . Candida albicans has an ability to form biofilms on denture base materials and to cause denture-induced stomatitis 41,42) . This study evaluated the effectiveness of three denture cleaning agents on the surface characteristic and also adhesion of Candida albicans.…”