A comparative light and electron microscopical study of compatible and incompatible interactions between Xanthomonas campestris pv. campestris and cabbage (Brassica oleracea)

Bretschneider, Karin E.; Gonella, M. P.; Robeson, David J.

doi:10.1016/0885-5765(89)90026-x

Cited by 39 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B-4362 were more intensively symbiotic (Sequeira et al, 1977; Djordjevic, Gabriel immunogold-labelled than those in cv. SP 70-114370- , & Rolfe, 1987Bretschneider et al, 1989;Brewin, suggesting that the production of the bacterial mucus 1991; Leigh & Coplin, 1992;McKhann & Hirsch, recognized by the antibody w^as greatly enhanced 1994; Perotto et al, 1994;Young et al, 1995). in the susceptible cultivar.…”

Section: Light and Electron Microscopy Antibody Preparation And Immumentioning

confidence: 99%

Infection of mottled stripe disease‐susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirilium

et al. 1997

View full text Add to dashboard Cite

SUMMARYLeaves of a mottled stripe disease-susceptible cultivar (B-4362) and of a mottled stripe disease-resistant cultivar (SP 70-1143) of sugar cane (interspecific hybrids of Saccharum) were inoculated with the diazotrophic endophytes, Herbaspirillum rubrisubalbicans or Herbaspir ilium seropedicae, via injection into the apex of the stem. At 7 and 20 d.a.i., H. seropedicae could be isolated only from a small necrotic area around the point of inoculation, where there was considerable degradation of host cells, and was not detected in any other part of the leaves. This suggested a hypersensitive response by the host to this bacterium, and no disease symptoms formed on either cultivar. By contrast, H. rubrisubalbicans could be re-isolated from throughout the infected leaves of both cultivars at both harvests and produced widespread disease symptoms on the leaves of cv. B-4362.Symptoms consisted of necrotic regions near the point of inoculation, and red stripes and red patches along the vertical axis of the leaves, where the bacteria had spread in the primary and secondary veins. The xylemconducting elements in diseased regions of leaves were filled with bacteria and, at the edges of disease symptoms, the vessels were filled with a gum which stained blue-green with toluidine blue. This material probably contained phenolic compounds ,and was produced as a host defence response. Leaves of cv. SP 70-1143 only developed small red stripes near the point of inoculation. These symptoms did not spread along the leaves, and the infected xylem vessels were never seen to be completely full of bacteria. Instead, the vessels contained encapsulated bacterial colonies attached to secondary wall deposition; these colonies were surrounded by blue-green material that might have been host-defence gums. In cv. B-4362, bacteria were abundant in the intercellular spaces of mesophyll adjacent to infected xylem, and also filled sub-stomatal cavities. Immunogold labelling using polyclonal antisera raised against H. rubrisubalbicans gave a weak signal with the bacteria in cv. SP 70-1143, showing that few binding sites were available to the antibodies. By contrast, bacteria in cv. B-4362 reacted strongly with the antibody, suggesting that they had a denser coating of immunoreactive mucus. In the later stages of infection of cv. B-4362, lysed bacteria were seen within degraded plant cells surrounded by a matrix of plant gums and bacterial mucus. This matrix reacted strongly to the H. rubrisubalbicans antibodies. Immunogold labelling using antibodies against nitrogenase component II showed that nitrogenase was expressed by bacteria in the early stages of infection of cv. B-4362, but not in later stages, or by bacteria infecting cv. SP 70-1143.

show abstract

Section: Light and Electron Microscopy Antibody Preparation And Immumentioning

confidence: 99%

Infection of mottled stripe disease‐susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirilium

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Vegetables may already be infected at harvest even though they do not show visible symptoms (Bhat et al 2012). Due to the ability to cause latent infection, the appearance of symptoms is strongly dependent on environmental conditions (Bretschneider et al 1989;Czajkowski et al 2011). However, postharvest bacterial soft rot losses caused by various species of Bacillus, Pseudomonas and Erwinia have been estimated to vary between 15-30% of the harvested crop (Agrios 2006).…”

Section: Introductionmentioning

confidence: 99%

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Eichmeier¹,

Peňázová²,

Pecenka³

et al. 2016

Journal of Plant Protection Research

View full text Add to dashboard Cite

Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three diff erent parts of the cabbage heads. Isolations were carried out from stalks, inner and superfi cial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identifi ed according to the sequences obtained. Th e most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identifi ed. Th e results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.

show abstract

“…In some interactions, the nature of the constitutive compounds of such material was investigated cytologically, revealing the presence of pectic elements, callose or lignin-like molecules (Bretschneider et al 1989;Boher et al 1996;Kpémoua et al 1996;Rioux et al 1998). The present study was conducted to provide cytochemical information on vessel occlusion regarding the involvement of tyloses and gels in defense responses of the plane tree to Cfp.…”

Section: Alain Clérivet · Véronique Déon · Ibtissam Alami Fredérique mentioning

confidence: 99%

Tyloses and gels associated with cellulose accumulation in vessels are responses of plane tree seedlings (Platanus × acerifolia) to the vascular fungus Ceratocystis fimbriata f. sp platani

Clérivet

Déon

Alami

et al. 2000

Trees

View full text Add to dashboard Cite

Stems of plane tree seedlings were cytologically investigated regarding the involvement of tyloses and gels in defense reactions to Ceratocystis fimbriata f. sp platani, the canker stain disease agent. In the lumen of infected xylem vessels, cytochemical tests revealed the occurrence of compounds labeled for cellulose and which seem to be involved in gel formation. Immunogold methods indicated that pectin-rich material accumulated in the paramural area of vessel-associated parenchyma cells and around tylosis walls. Tylosis formation and deposition of pectin-containing gels were associated with metabolic changes of vessel-associated parenchyma cells. It is suggested that parenchyma cells play a key role in the defense of plane tree seedlings through plugging of xylem vessels leading to compartmentalization of the vascular fungus.

show abstract

A comparative light and electron microscopical study of compatible and incompatible interactions between Xanthomonas campestris pv. campestris and cabbage (Brassica oleracea)

Cited by 39 publications

References 24 publications

Infection of mottled stripe disease‐susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirilium

Infection of mottled stripe disease‐susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirilium

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Tyloses and gels associated with cellulose accumulation in vessels are responses of plane tree seedlings (Platanus × acerifolia) to the vascular fungus Ceratocystis fimbriata f. sp platani

Contact Info

Product

Resources

About