A comparative study between lignin down regulated alfalfa lines and their respective unmodified controls on the nutritional characteristics of hay

Getachew, G.; Ibáñez, Ana María; Pittroff, W.; Dandekar, Abhaya M.; McCaslin, Mark; Goyal, Sneh; Reisen, Peter; DePeters, E.J.; Putnam, Daniel H.

doi:10.1016/j.anifeedsci.2011.09.009

Cited by 28 publications

(38 citation statements)

References 36 publications

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“…Reddy et al (2005) reported a strong negative linear relationship between in situ digestibility and ADL levels across all reduced lignin lines. In addition to increased digestibility, reduced lignin alfalfa populations have also shown reduced NDF concentrations and greater non-fiber carbohydrate concentrations compared to control lines (Guo et al, 2001b;Reddy et al, 2005;Getachew et al, 2011;Li et al, 2015a), while CP concentrations remained similar for reduced lignin and reference alfalfa lines (Getachew et al, 2011;Li et al, 2015a).…”

Section: Introductionmentioning

confidence: 98%

“…Experimental populations of COMT and/or CCOMT downregulated alfalfa have shown a 4 to 29% decrease in stem lignin concentration and a 1 to 24% decrease in herbage lignin concentration compared to reference alfalfa cultivars (Guo et al, 2001a;2001b;Marita et al, 2003;Reddy et al, 2005;Undersander et al, 2009;Getachew et al, 2011). The wide variation in lignin reduction reported could be due to the specific downregulated gene (Guo et al, 2001a(Guo et al, , 2001bMarita et al, 2003;Undersander et al, 2009;Getachew et al, 2011), the methods used for lignin analysis (Guo et al, 2001a;2001b;Jung et al, 2012), or the plant growing conditions (Baucher et al, 1999).…”

Section: Introductionmentioning

confidence: 98%

“…Populations of reduced lignin alfalfa have shown an increase in in vitro DMD, in situ rumen digestibility, and in vitro NDFD (Guo et al, 2001b;Reddy et al, 2005;Mertens and McCaslin, 2008;Weakley et al, 2008;Undersander et al, 2009;Getachew et al, 2011). Reddy et al (2005) reported a strong negative linear relationship between in situ digestibility and ADL levels across all reduced lignin lines.…”

Section: Introductionmentioning

confidence: 99%

“…Several experimental lines of alfalfa have been developed with downregulation of the caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) lignin biosynthetic genes (Inoue et al, 1998;Guo et al, 2001a;Marita et al, 2003;Getachew et al, 2011). Experimental populations of COMT and/or CCOMT downregulated alfalfa have shown a 4 to 29% decrease in stem lignin concentration and a 1 to 24% decrease in herbage lignin concentration compared to reference alfalfa cultivars (Guo et al, 2001a;2001b;Marita et al, 2003;Reddy et al, 2005;Undersander et al, 2009;Getachew et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Forage Accumulation and Nutritive Value of Reduced Lignin and Reference Alfalfa Cultivars

et al. 2017

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Core Ideas Reduced lignin and reference cultivars did not differ in forage accumulation. Forage accumulation was greater with a fall harvest or a 40‐d cutting schedule. Reduced lignin alfalfa averaged 8% less acid detergent lignin and 10% greater neutral detergent fiber digestibility. Cutting treatments with shorter harvest intervals increased forage nutritive value. Delaying reduced lignin alfalfa harvest increased forage mass and maintained quality. Reduced lignin alfalfa (Medicago sativa L.) cultivars have the potential to increase the feeding value of alfalfa for livestock by improving forage fiber digestibility and to increase harvest management flexibility. The objectives were to compare the forage accumulation and nutritive value of reduced lignin and reference alfalfa cultivars when subject to diverse cutting treatments in the establishment and first production year. Research was established in 2015 at four locations in Minnesota. Reference alfalfa cultivars 54R02, DKA43–22RR, WL 355.RR, and the reduced lignin cultivar 54HVX41 were subject to cutting treatments with variable intervals between harvests. Cultivar by cutting treatment interactions were not significant (P > 0.05), but cultivar and cutting treatment effects were significant. Cultivars did not consistently differ in forage accumulation. Establishment year forage accumulation was greater when a fall harvest was taken, and first production year forage accumulation was generally greatest when alfalfa was harvested on a 40‐d cutting schedule. Compared to reference alfalfa cultivars, 54HVX41 had an average of 8% less acid detergent lignin (ADL) and 10% greater neutral detergent fiber digestibility (NDFD) but was similar in crude protein (CP) and neutral detergent fiber (NDF) concentrations. Cutting treatments with shorter harvest intervals increased forage CP and NDFD and decreased NDF and ADL concentrations. With a 5‐d harvest delay, 54HVX41 harvested on a 35‐d harvest interval had a 21% gain in forage mass and a 3% reduction in relative forage quality (RFQ) compared to reference cultivars harvested on a 30‐d harvest interval, which could allow for increased management flexibility.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Forage Accumulation and Nutritive Value of Reduced Lignin and Reference Alfalfa Cultivars

et al. 2017

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“…This has been achieved through the genetic modification of the lignin biosynthetic pathway by either reducing lignin concentrations or altering lignin composition. These modifications have improved forage digestibility in vitro (Getachew et al 2011). The most successful approach has been to alter lignin content rather than lignin composition in alfalfa (Zhao and Dixon 2014).…”

Section: Gene Technologymentioning

confidence: 99%

Breaking through the feed barrier: options for improving forage genetics

et al. 2015

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Abstract. Pasture based on perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.) is the foundation for production and profit in the Australasian pastoral sectors. The improvement of these species offers direct opportunities to enhance sector performance, provided there is good alignment with industry priorities as quantified by means such as the forage value index. However, the rate of forage genetic improvement must increase to sustain industry competitiveness. New forage technologies and breeding strategies that can complement and enhance traditional approaches are required to achieve this. We highlight current and future research in plant breeding, including genomic and gene technology approaches to improve rate of genetic gain. Genomic diversity is the basis of breeding and improvement. Recent advances in the range and focus of introgression from wild Trifolium species have created additional specific options to improve production and resource-use-efficiency traits. Symbiont genetic resources, especially advances in grass fungal endophytes, make a critical contribution to forage, supporting pastoral productivity, with benefits to both pastures and animals in some dairy regions. Genomic selection, now widely used in animal breeding, offers an opportunity to lift the rate of genetic gain in forages as well. Accuracy and relevance of trait data are paramount, it is essential that genomic breeding approaches be linked with robust field evaluation strategies including advanced phenotyping technologies. This requires excellent data management and integration with decision-support systems to deliver improved effectiveness from forage breeding. Novel traits being developed through genetic modification include increased energy content and potential increased biomass in ryegrass, and expression of condensed tannins in forage legumes. These examples from the wider set of research emphasise forage adaptation, yield and energy content, while covering the spectrum from exotic germplasm and symbionts through to advanced breeding strategies and gene technologies. To ensure that these opportunities are realised on farm, continuity of industry-relevant delivery of forage-improvement research is essential, as is sustained research input from the supporting pasture and plant sciences.

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Stem and leaf forage nutritive value and morphology of reduced lignin alfalfa

Grev

Wells

Catalano³

et al. 2020

Agronomy Journal

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Reduced lignin alfalfa (Medicago sativa L.) has the potential to improve alfalfa forage quality, yet forage morphology, biomass allocation, and stem and leaf forage nutritive value remains undetermined. The objectives of this study were to characterize changes in morphological development and forage nutritive value within stem and leaf fractions for reduced lignin alfalfa. Reduced lignin (‘54HVX41’) and reference (‘DKA43‐22RR’) alfalfa cultivars were sampled weekly from the mid‐vegetative stage through full flower during the seeding (2015) and first production (2016) year at two locations in Minnesota. Samples were staged to determine maturity, divided into stem and leaf fractions, weighed, and analyzed for forage nutritive value. Alfalfa cultivars were similar in stem and leaf yield, leaf to stem ratio (L/S), stem crude protein (CP), stem neutral detergent fiber (NDF), leaf CP, leaf NDF, leaf acid detergent lignin (ADL), and leaf NDF digestibility (NDFD). On average, reduced lignin alfalfa showed a 14% decrease in stem ADL and a 12% increase in stem NDFD compared to reference alfalfa. For both cultivars, increases in forage maturity increased stem and leaf yields and decreased L/S. Forage maturity also affected stem and leaf forage nutritive values; as maturity increased, NDF and ADL increased while CP and NDFD decreased. These results indicate that changes in forage nutritive value for reduced lignin alfalfa are largely a result of changes in ADL and NDFD within the stem fraction, and confirm that alfalfa forage nutritive value is negatively affected by forage maturity, particularly within the stem portion of the plant.

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A comparative study between lignin down regulated alfalfa lines and their respective unmodified controls on the nutritional characteristics of hay

Cited by 28 publications

References 36 publications

Forage Accumulation and Nutritive Value of Reduced Lignin and Reference Alfalfa Cultivars

Forage Accumulation and Nutritive Value of Reduced Lignin and Reference Alfalfa Cultivars

Breaking through the feed barrier: options for improving forage genetics

Stem and leaf forage nutritive value and morphology of reduced lignin alfalfa

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