A comparative study of a label-free DNA capacitive sensor using a pyrrolidinyl peptide nucleic acid probe immobilized through polyphenylenediamine and polytyramine non-conducting polymers

Sankoh, Supannee; Samanman, Saluma; Thipmanee, Orawan; Numnuam, Apon; Limbut, Warakorn; Kanatharana, Proespichaya; Vilaivan, Tirayut; Thavarungkul, Panote

doi:10.1016/j.snb.2012.11.077

Cited by 21 publications

(13 citation statements)

References 35 publications

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“…The acpcPNA probes are also used to achieve a highly sensitive label-free capacitive DNA sensor (Thipmanee et al, 2012). A comparative study has been reported on the performance of a label-free capacitive DNA sensor making the use of the pyrrolidinyl PNA probe which is immobilized through the polyphenylenediamine and the polytyramine nonconducting polymers (Sankoh et al, 2013). Numerous PNA probes combined with nanomaterials have also been designed to develop biosensors.…”

Section: Application In Sensor Probementioning

confidence: 99%

Evolution of peptide nucleic acid with modifications of its backbone and application in biotechnology

Das

Pradhan

2020

Chem Biol Drug Des

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Peptide nucleic acids (PNAs) are getting prodigious interest currently in the biomedical and diagnostic field as an extremely powerful tool because of their potentiality to hybridize with natural nucleic acids. Although PNA has strong affinity and sequence specificity to DNA/RNA, there is a considerable ongoing effort to further enhance their special chemical and biological properties for potential application in numerous fields, notably in the field of therapeutics. The toolbox for backbone modified PNAs synthesis has been extended substantially in recent decades, providing a more efficient synthesis of peptides with numerous scaffolds and modifications. This paper reviews the various strategies that have been developed so far for the modification of the PNA backbone, challenging the search for new PNA systems with improved chemical and physical properties lacking in the original aegPNA backbone. The various practical issues and limitations of different PNA systems are also summarized. The focus of this review is on the evolution of PNA by its backbone modification to improve the cellular uptake, sequence specificity, and compatibility of PNA to bind to DNA/RNA. Finally, an insight was also gained into major applications of backbone modified PNAs for the development of biosensors.

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Section: Application In Sensor Probementioning

confidence: 99%

Evolution of peptide nucleic acid with modifications of its backbone and application in biotechnology

Das

Pradhan

2020

Chem Biol Drug Des

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“…In contrast, biosensor systems based on electrochemical techniques, such as chronoamperometry [ 20 ], capacitance [ 21 , 22 ], and electrochemical impedance spectroscopy (EIS) [ 20 ], have been shown to be of great utility due to their sensibility and low cost [ 23 , 24 ]. By detecting changes that occur during hybridization at the interface between a DNA functionalized electrode and a conductive target analyte solution, electrochemical techniques have the potential to provide real-time measurement, label-free sensing, and more portable detection platforms [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

et al. 2021

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In this work, a low-cost and rapid electrochemical resistive DNA biosensor based on the current relaxation method is described. A DNA probe, complementary to the specific human papillomavirus type 16 (HPV-16) sequence, was immobilized onto a screen-printed gold electrode. DNA hybridization was detected by applying a potential step of 30 mV to the system, composed of an external capacitor and the modified electrode DNA/gold, for 750 µs and then relaxed back to the OCP, at which point the voltage and current discharging curves are registered for 25 ms. From the discharging curves, the potential and current relaxation were evaluated, and by using Ohm’s law, the charge transfer resistance through the DNA-modified electrode was calculated. The presence of a complementary sequence was detected by the change in resistance when the ssDNA is transformed in dsDNA due to the hybridization event. The target DNA concentration was detected in the range of 5 to 20 nM. The results showed a good fit to the regression equation ΔRtotal(Ω)=2.99 × [DNA]+81.55, and a detection limit of 2.39 nM was obtained. As the sensing approach uses a direct current, the electronic architecture of the biosensor is simple and allows for the separation of faradic and nonfaradaic contributions. The simple electrochemical resistive biosensor reported here is a good candidate for the point-of-care diagnosis of HPV at a low cost and in a short detection time.

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“…Consequently, different immobilization layers have been used for the fabrication of biosensor gold sensor chip surfaces. The most reported immobilization layers for the fabrication of biosensor gold sensor chip surfaces are both conducting polymers, for example, polypyrrole [1][2][3][4], and non-conducting polymers, for example, polytyramine (Pty) [5][6][7][8][9][10]; as well as self-assembled monolayers (SAMs) of mercapto-containing organosilanes [11][12][13][14][15][16] and organosulfur compounds [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…While polymers are characterized by their good insulation, making them useful for capacitive systems, SAMs are well known for their orderly self-organizing behavior, maximizing the availability of the active groups for the immobilization of bio-recognition molecules. Although both non-conducting polymers (polytyramine) and hydroxyl-terminated SAMs (6-mercaptohexanol) have been extensively used as sensor chip modification layers in capacitive biosensor systems [5][6][7][8][9][10], there are no comprehensive studies comparing the two layers to document/establish sound scientific evidence of the superiority/suitability of one layer over the other for capacitive DNA sensor measurements. Such a comprehensive comparison study will provide sound scientific guidance in selecting a sensor chip modification layer for capacitive biosensor systems, achieving ultrasensitive clinical assays.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison

Mahadhy

Mattìasson

Ståhl-Wernersson

et al. 2021

Sensors

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The performance of a biosensor is associated with the properties of an immobilization layer on a sensor chip. In this study, gold sensor chips were modified with two different immobilization layers, polytyramine film and 6-mercaptohexanol self-assembled monolayer. The physical, electrochemical and analytical properties of polytyramine film and mercaptohexanol self-assembled monolayer modified gold sensor chips were studied and compared. The study was conducted using atomic force microscopy, cyclic voltammetry and a capacitive DNA-sensor system (CapSenze™ Biosystem). The results obtained by atomic force microscopy and cyclic voltammetry indicate that polytyramine film on the sensor chip surface possesses better insulating properties and provides more spaces for the immobilization of the capture probe than a mercaptohexanol self-assembled monolayer. A capacitive DNA sensor hosting a polytyramine single-stranded DNA-modified sensor chip displayed higher sensitivity and larger signal amplitude than that of a mercaptohexanol single-stranded DNA-modified sensor chip. The linearity responses for polytyramine single-stranded DNA- and mercaptohexanol single-stranded DNA-modified sensor chips were obtained at log concentration ranges, equivalent to 10−12 to 10−8 M and 10−10 to 10−8 M, with detection limits of 4.0 × 10−13 M and 7.0 × 10−11 M of target complementary single-stranded DNA, respectively. Mercaptohexanol single-stranded DNA- and polytyramine single-stranded DNA-modified sensor chips exhibited a notable selectivity at an elevated hybridization temperature of 50 °C, albeit the signal amplitudes due to the hybridization of the target complementary single-stranded DNA were reduced by almost 20% and less than 5%, respectively.

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A comparative study of a label-free DNA capacitive sensor using a pyrrolidinyl peptide nucleic acid probe immobilized through polyphenylenediamine and polytyramine non-conducting polymers

Cited by 21 publications

References 35 publications

Evolution of peptide nucleic acid with modifications of its backbone and application in biotechnology

Evolution of peptide nucleic acid with modifications of its backbone and application in biotechnology

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison

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