A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication

Smýkal, Petr; Jovanović, Ž.; Stanisavljević, Nemanja; Zlаtković, Bojаn; Ćupina, Branko; Đorđević, Vuk; Mikić, Aleksandar; Medović, Aleksandar

doi:10.1007/s10722-014-0128-z

Cited by 22 publications

(17 citation statements)

References 61 publications

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“…Smýkal et al (2011) studied 4429 Pisum accessions from three large world germplasm collections that include both wild and domesticated pea and found that introgression from wild species has been common and much of the diversity still resides in wild material and could be used further in breeding. Smýkal et al (2014), analysing ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. placed the ancient sample at an intermediate position between extant cultivated Pisum sativum L. and wild P. sativum subsp.…”

Section: Introductionmentioning

confidence: 99%

Analysis of variability and phylogeny in pisum (Pisum spp.) using digital phenotyping and morphological traits

Gatti¹,

Guindón²,

Bermejo³

et al. 2017

Aust J Crop Sci

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Plant phenotyping links genomics with plant ecophysiology and agronomy. It is usually performed by non-destructive, automated and image-based technology and generates information for efficient and searchable digital characterization of crop that can be performed during routine, periodical regeneration of accessions in germplasm collections. In the present work, ninety-two accessions of Pisum from different species and subspecies were studied during 2015 and 2016. Size and colour traits were measured using digital images from a Samsung CLX 3300 scanner and analysed with appropriate software; also seed weight, plant height and days to flowering were measured. Highly significant differences between accessions and species and subspecies for all these traits were found. When distances among species and subspecies are calculated, P. sativum subsp. sativum showed the greatest distance with P. fulvum (8.02) followed by P. abyssinicum (7.13); while the smallest distance was found between P. fulvum and P. sativum subsp. transcaucasicum (3.16). A Neighbour-joining tree with a cofenetic r of 0.985 was obtained. Seed and pod characteristics as colour parameters and size, obtained by digital phenotyping, have proved to be suitable markers for genetic diversity evaluation and they are useful in evolutionary analysis, allowing the discrimination of the main wild and cultivated species in the genus Pisum.Keywords: Pisum, seed coat colour, origin, variability, phenotyping, legumes. Abbreviations: a_and b-colour coordinates from the CieLab system of colour, C_ calibre of grains in centimetres, CR_ coincidence rate, CV%_ variation coefficient, DF_ days until 50% of plants flowering, L_ psychometric index of lightness from the CieLab system of colour; Max_ maximum , MD%_ mean difference percentage , Min_ minimum, PD_ pod diameter in centimetres, PH_ plant height in centimetres, PL_ pod length in centimetres, RBIP_ retrotransposon-based insertion polymorphisms markers, RGB_ red, green and blue, SD_ standard deviation , SSAP_ sequence-specific amplification polymorphism markers, VD%_ variance difference percentage, VR%_ variable rate of coefficient of variance, W100S_ weight of 100 seeds in grams.

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Section: Introductionmentioning

confidence: 99%

Analysis of variability and phylogeny in pisum (Pisum spp.) using digital phenotyping and morphological traits

Gatti¹,

Guindón²,

Bermejo³

et al. 2017

Aust J Crop Sci

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“…The precise area of its origin is difficult to specify because of its early cultivation [159]. It is probably Abyssinia and Afghanistan [160].…”

Section: The Most Common Grain Legumes As Cover Cropsmentioning

confidence: 99%

Molecular, Genetic and Agronomic Approaches to Utilizing Pulses as Cover Crops and Green Manure into Cropping Systems

Tani

Abraham

Chachalis

et al. 2017

IJMS

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Cover crops constitute one of the most promising agronomic practices towards a more sustainable agriculture. Their beneficial effects on main crops, soil and environment are many and various, while risks and disadvantages may also appear. Several legumes show a high potential but further research is required in order to suggest the optimal legume cover crops for each case in terms of their productivity and ability to suppress weeds. The additional cost associated with cover crops should also be addressed and in this context the use of grain legumes such as cowpea, faba bean and pea could be of high interest. Some of the aspects of these grain legumes as far as their use as cover crops, their genetic diversity and their breeding using conventional and molecular approaches are discussed in the present review. The specific species seem to have a high potential for use as cover crops, especially if their noticeable genetic diversity is exploited and their breeding focuses on several desirable traits.

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“…Probably the first successful extraction of ancient DNA (aDNA) from the charred legume seeds was done from the bitter vetch and pea samples from the site of Hissar in southeast Serbia Smýkal et al 2014) (Fig. 4).…”

Section: Palaeogeneticsmentioning

confidence: 99%

Presence of vetches (Vicia spp.) in agricultural and wild floras of ancient Europe

Mikić

2016

Genet Resour Crop Evol

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Vetches (Vicia spp.) were part of the everyday diet of the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. Among the major criteria to determine the domestication in vetches and other ancient grain legumes are nondehiscent pods, larger seed size and smooth seed coat. The seeds of bitter vetch [V. ervilia (L.) Willd.] were found among both the earliest findings of wild collected plants from Early Pre-Pottery Neolithic, from 12th millennium BC cal and the storages of domesticated crops of the Near East Neolithic. Vetches entered Europe in its south-east regions and progressed into its interior via Danube. Archaeological findings offer nice examples that confirm the importance vetches had in the primeval agriculture in Europe and its adjacent regions. Apart from the samples of cultivated vetches used either for food or feed or both, there is rich archaobotanical evidence on the wild and weedy vetch species in diverse European ecosystems. Recently the first known success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The future research on this subject certainly must make a more detailed map of the paths of the vetch distribution over Europe and, especially, its long-term and essentially important ties with their domestication and distribution in Asia Minor, Near East and North Africa. The initiated research on the vetch ancient DNA should bring more light onto the individual steps of the earliest days of vetch crops. The preliminary historical linguistic analysis assessed two Proto-Indo-European roots associated with vetches, *erEg w (h)-denoting pea, and *weik-meaning to avoid, initially gave the Latin vincīre, meaning to bind, and then vicia, denoting vetches in general. This multidisciplinary approach will hopefully be a useful reminder how widespread and important vetches used to be, as well as a tool for their re-introduction as presently neglected crops into the contemporary European agriculture.

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A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication

Cited by 22 publications

References 61 publications

Analysis of variability and phylogeny in pisum (Pisum spp.) using digital phenotyping and morphological traits

Analysis of variability and phylogeny in pisum (Pisum spp.) using digital phenotyping and morphological traits

Molecular, Genetic and Agronomic Approaches to Utilizing Pulses as Cover Crops and Green Manure into Cropping Systems

Presence of vetches (Vicia spp.) in agricultural and wild floras of ancient Europe

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