A Comparative Study of Detection of Serum Total and Direct Bilirubin by Manual and Automated Methods

Abstract

Background: Bilirubin is one of the major bile Pigment, clinically and biologically important among the bile pigments present in the mammals. Material and Methods; The Cross sectional study was conducted in department of biochemistry in the collaboration with department medicine in Civil Hospital Tarn Taran. Out of 90 patients, 54 were males and 36 were females. Result; The comparison of Serum Total and Direct bilirubin by Jendrassik & Grof and Automated method was Non- Significant with p value = 0.7788 and 0.5394 respectively. The comparison of Serum Total and Direct bilirubin by Malloy Evelyn and Automated method is Highly Significant with p value =0.0002 and 0.0003 respectively. The mean difference of malloy with jendrassik & grof and automated was 2.394 and 2.162 respectively and the mean difference of jendrassik & grof with automated method was -0.231. Conclusion; It was concluded that a good correlation is found between bilirubin detection by manual and automated method. Non- significant mean difference between Jendrassik & Grof and Automated makes it better than Malloy Evelyn.

Protective effects of ethanol-water leaf extract of Jatropha gossypiifolia on mouse virus-induced hepatitis (MHV) in male Swiss mice (Mus musculus)

“…The determination of serum bilirubin was performed according to the method described by Jagroop et al [18]. Total bilirubin reacts with 2,4-dichloroaniline to form azobilirubin.…”