A comparative study of different diets to optimize cultivation of Hyalella azteca in the laboratory

Fracácio, Renata; Dias, Geisa Soares; Zagatto, Pedro Antônio; Bidinotto, Paulo Mauricio; Silva, Paulo

doi:10.1016/j.ecoenv.2011.05.013

Cited by 3 publications

(8 citation statements)

References 6 publications

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“…The smallest number was three offsprings (formulation 4B), and the greatest number was 11 offsprings (formulations 4C, 5A, and 5D). These results are similar to those of Fracácio et al (2011), who found about 7 to 11 offsprings per couple aging between 30 to 42 days. Kruskal-Wallis analysis of variance test resulted in p > 0.05 for formulations 4 and 5 and between both formulations, therefore they suggest that there were no significant differences between the varied treatments in terms of numbers of produced offspring.…”

Section: Resultssupporting

confidence: 80%

“…The ecotoxicological studies were performed using the Hyalella azteca species cultivated in incubators (Tecnal EX80) on controlled conditions of temperature (24 ± 1 °C) and photoperiod (12 hours -light, 12 hoursdark), as recommended by ABNT NBR 15470 (ABNT, 2013) and USEPA (2000) for evaluation of growth and reproduction, respectively. Organisms' feeding was in accordance with Fracácio et al (2011). The test organisms were fed every day in the experimental period with a mix of 10 mg/mL fish food solution (TetraMin®), 5 mg/mL yeast and 0.1 mL/100 mL primrose oil (0,025 mL/organism/day) and macerated flakes of fish food containing Spirulina (TetraVeggie® Spirulinaenhanced flakes, 0.2 mg/organism/every other day).…”

Section: Suitability Of Sediments For Use In Ecotoxicological Studiesmentioning

confidence: 99%

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Formulation of sediments with characteristics of tropical lotic systems for use in ecotoxicological assays

Arine¹,

Fracácio²

2016

Rev. Bras. Ciênc. Ambient. (Online)

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Investigation of the dynamics and toxicity of pollutants in natural sediments is hindered by the great complexity of their matrix. Given this difficulty, the objective of this paper was to recommend the formulation of sediments representative of tropical lotic environments, considering their use in ecotoxicological assays. Organic and inorganic fractions were used in different percentages, resulting in five sediment formulations with different grain size proportions (fine, medium and coarse sand, and clay). Measured dissolved oxygen and hardness values were suitable for ecotoxicological assays; however, regarding pH values, only formulations with lower levels of organic matter provided suitable water quality for this purpose (between 6.3 and 7.3), for up to 10 days. These formulations were tested using Hyalella azteca, which confirmed their suitability for use in toxicological tests, since no lethal effects were observed and there were no significant changes in terms of growth and reproduction of the organisms.Keywords: formulated sediments; ecotoxicological assays; Hyalella azteca; tropical water bodies. RESUMOOs estudos relacionados à dinâmica de poluentes e toxicidade em sedimentos naturais são de difícil compreensão por se tratar de uma matriz muito complexa. Diante dessa problemática, o objetivo deste trabalho foi propor a formulação de sedimentos com características representativas de ambientes lóticos tropicais, considerando-se a sua aplicação em ensaios ecotoxicológicos. As frações orgânica e inorgânica foram utilizadas em porcentagens variadas, totalizando cinco formulações de sedimento com diferentes proporções granulométricas (areia fina, média, grossa e argila). Os resultados de oxigênio dissolvido e dureza permaneceram adequados para ensaios ecotoxicológicos, porém, considerando-se o pH, apenas as formulações contendo menores taxas de matéria orgânica apresentaram-se adequadas para este fim (entre 6,3 e 7,3), num período de estabilização de até 10 dias. A espécie Hyalella azteca confirmou a aplicabilidade desses sedimentos em testes toxicológicos, não tendo sido observadas taxas de letalidade, tampouco variações significativas em relação aos parâmetros de crescimento e reprodução dos organismos.Palavras-chave: sedimentos formulados; ensaios ecotoxicológicos; Hyalella azteca; corpos de água tropicais.

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Section: Resultssupporting

confidence: 80%

Section: Suitability Of Sediments For Use In Ecotoxicological Studiesmentioning

confidence: 99%

Formulation of sediments with characteristics of tropical lotic systems for use in ecotoxicological assays

Arine¹,

Fracácio²

2016

Rev. Bras. Ciênc. Ambient. (Online)

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“…The food quality of each of the diets varied, with the FF diet having the highest overall protein, fat, and carbohydrate content, whereas the diatom slurry had the lowest protein, fat, and carbohydrate contents. Fraćacio et al completed a diet study with H. azteca fed 4 different diets and also found survival differences to be nonsignificant between differing diets. One of the diets Fraćacio et al tested was comprised of a fish food solution, yeast, primrose oil, rabbit food, and spirulina fish flakes that had significantly higher reproduction and the largest growth.…”

Section: Discussionmentioning

confidence: 99%

“…Fraćacio et al completed a diet study with H. azteca fed 4 different diets and also found survival differences to be nonsignificant between differing diets. One of the diets Fraćacio et al tested was comprised of a fish food solution, yeast, primrose oil, rabbit food, and spirulina fish flakes that had significantly higher reproduction and the largest growth. This diet was high in animal protein, as well as vitamins, minerals, fatty acids, and gama‐linolenic .…”

Section: Discussionmentioning

confidence: 99%

“…Fraćacio et al compared 4 different diets for H. azteca that are commonly used in international standard methods and examined the effects the diet had on survival, length, weight, and reproductive success. It was found that the different diets offered varying success in different aspects of the study (growth, survival, reproduction), indicating that there is not yet 1 diet that can be considered optimal for the entire life cycle of the species.…”

Section: Introductionmentioning

confidence: 99%

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The evaluation of 3 diets for rearingHyalella aztecaand the influence of diet on acute ammonia toxicity

Kennedy

Watson-Leung

Poirier

2016

Enviro Toxic and Chemistry

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Three Hyalella azteca cultures were reared on different diets since birth, reflecting the recommended diets of various investigators. The 3 diets consisted of fish flakes (FF), a mixture of fish flakes supplemented with the diatom Thalassiosira weissflogii (FF-D), and a mixture of fish flakes supplemented with yeast, cereal grass media, and trout chow (FF-YCT). The 3 diets were evaluated by comparing 20 wk of culturing data, along with the organism's response to standard 96-h toxicity testing with ammonium chloride over a range of pH and temperature. Hyalella azteca fed the FF-D diet had the highest overall survival rate (96.6%, standard deviation [SD] 4.3%) compared to those fed the FF diet (92.0%, SD 12.7%), or the FF-YCT diet (91.1%, SD 14.8%), although difference in survival was not statistically significant. Organisms fed the FF-D diet produced a higher number of young per week per adult (6.1, SD 2.8) than the FF diet (5.1, SD 2.2), or the FF-YCT diet (4.0, SD 1.2), although differences were not statistically significant. Of the diets evaluated, H. azteca reared on the FF-D diet were often significantly more resistant to total and un-ionized ammonia toxicity in acute 96-h testing than those reared on the other 2 diets across the 2 temperatures and 5 pHs tested, suggesting this may be the most optimal diet for this species. Environ Toxicol Chem 2016;35:2416-2424. © 2016 SETAC.

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Evaluating the Effects of Diet on the Sensitivity of Hyalella azteca to an “Eco‐friendly” Deicing Agent

Kohler,

Yates,

Tomlinson

et al. 2024

Enviro Toxic and Chemistry

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Salting of roadways contaminates local waterways via snowmelt and precipitation runoff, eliciting various toxicological impacts on aquatic ecosystems. Recently, “eco‐friendly” deicing alternatives have been introduced in hopes of mitigating environmental impacts of deicing agents, while maintaining human safety. These “eco‐friendly” alternatives may pose their own set of environmental concerns that require further study. While the potential toxicity of road salts has been evaluated for various aquatic species, the environmental factors that may influence this toxicity are less understood; and for emerging deicing alternatives, there is a lack of literature documenting these potential implications. For aquatic organisms, the highest exposure to road salts may coincide with reduced food availability, namely during the winter months. The present study evaluates the effect of a conditioning diet on the sensitivity of adult Hyalella azteca to an “eco‐friendly”‐labeled beet deicer (Snow Joe MELT Beet‐IT). Various conditioning diets were examined, including TetraMinTM, TetraMin and diatom (Thalassiosira weissflogii) combinations, and TetraMin and conditioned Acer sacharum leaves. For each diet type, 48‐ and 96‐h water‐only toxicity bioassays were conducted with adult H. azteca. These results were compared to organisms which experienced a 96‐h starvation period prior to exposure and culture organisms. Diet types representing excess quality and quantity of food significantly decreased the toxicity of beet deicer to the organisms. However, starvation likely increases the toxicity of road salts to H. azteca. Therefore, the quantity and quality of food available to H. azteca may influence their sensitivity to deicing agents. Environ Toxicol Chem 2024;43:2608–2615. © 2024 SETAC

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A comparative study of different diets to optimize cultivation of Hyalella azteca in the laboratory

Cited by 3 publications

References 6 publications

Formulation of sediments with characteristics of tropical lotic systems for use in ecotoxicological assays

Formulation of sediments with characteristics of tropical lotic systems for use in ecotoxicological assays

The evaluation of 3 diets for rearingHyalella aztecaand the influence of diet on acute ammonia toxicity

Evaluating the Effects of Diet on the Sensitivity of Hyalella azteca to an “Eco‐friendly” Deicing Agent

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