A comparative study of different amberlite XAD resins in flavonoid analysis

Abstract

A comparative study of the recovery of flavonoids (both aglycones and glycosides) by different Amberlite XAD resins has been achieved. No significant differences in the percentages of recovery were found with the chemical nature and physical characteristics of the different resins. A flavonoid recovery higher than 90% is possible in a number of cases. The possibility of fractionating the adsorbed flavonoids by desorption with methanol: water mixtures has also been studied. The polystyrene resins (XAD‐2, XAD‐4 and XAD‐16) were found to be more useful for fractionation purposes than the polyacrylic resins (XAD‐7, XAD‐8). The results suggest that Amberlite XAD‐2 is the most suitable for the recovery and fractionation of flavonoids from plant extracts and foods rich in sugars and other polar compounds such as honey and fruit jams.

Flavonoids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication

“…The fluid samples were then filtered through cotton wool to remove the solid particles. The filtrate was mixed with 150 g of Amberlite XAD-2 (Supelco, Bellefonte, PA, USA, pore size 9 nm, particle size 0.3-1.2 mm) and stirred in a magnetic stirrer for 10 min, which was considered enough to absorb honey flavonoids with a recovery rate more than 80% (Martos et al, 1997; Tom as-Barber an, Blazquez, Garc ıa-Viguera, Ferreres, & Tom as-Lorent, 1992). The Amberlite particles were then packed in a glass column (42 Â 3.2 cm) and the column was washed with acidified water (pH 2 with HCl, 250 ml) and subsequently rinsed with distilled water (300 ml) to remove all sugars and other polar constituents of the honey.…”

Phenolic acids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication

“…The fluid samples were then filtered through cotton wool to remove the solid particles. The filtrate was mixed with 150 g Amberlite XAD-2 (Supelco, Bellefonte, PA, USA, pore size 9 nm, particle size 0.3-1.2 mm) and stirred in a magnetic stirrer for 10 min, which was considered enough to absorb honey phenolics (including flavonoids, phenolic acids and abscisic acid) with a recovery rate more than 80% (Martos et al, 1997;Tomás-Barberán, Blazquez, García-Viguera, Ferreres, & Tomás-Lorent, 1992;Yao et al, 2003Yao et al, , 2004a. The Amberlite particles were then packed in a glass column (42 · 3.2 cm) and the column was washed with acidified water (pH 2 with HCl, 250 ml) and subsequently rinsed with distilled water (300 ml) to remove all sugars and other polar constituents of the honey.…”

A simple extractive technique for honey flavonoid HPLC analysis

“…Liquid-liquid partitions produce inconvenient interphases which do not permit the complete recovery of flavonoids. This problem has recently been solved by using the non-ionic polymeric resin Amberlite XAD-2 (Ferreres et al, 1991;Tomás-Barberán et al, 1992). In a previous paper we reported the identification of 16 flavonoids in honey via HPLC analysis of samples prepared with a combination of Amberlite XAD-2 and Sephadex chromatography (Ferreres et al, 1991).…”