“…However, bare Au surfaces are not suitable for the biosensor because of its poor absorbance properties of biomolecules (Wu et al, 2010 ). Therefore, traditional biosensors are not capable to detect low molecular weight of biomolecules because of poor attachment of these biomolecules to the bare metal surface (Maurya and Prajapati, 2016 ). Until now, many methods have been developed to increase the sensitivity of SPR sensor such as using adhesion layer (Agarwal et al, 2016a , b ), metal nanoparticles and nanohole (Prasad et al, 2019 ), metal nanoslits (Yeung et al, 2018 ), and gold nanoparticles (Amendola et al, 2017 ).…”