A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

Tomsia, Marcin; Nowicka, Joanna; Skowronek, Rafał; Woś, Magdalena; Wójcik, Joanna; Droździok, Kornelia; Zorychta, Magdalena; Javan, Gulnaz T.; Chełmecka, Elżbieta

doi:10.3390/pr8121637

Cited by 8 publications

(12 citation statements)

References 28 publications

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“…The ethanol concentration ratio between the blood and urine of the deceased with the confirmed ethanol intoxication was 0.83, 0.89, and 0.70, respectively for Case 1, Case 3, and Case 6. Our earlier study on unembalmed cadavers (n = 39) showed that the average blood/urine ethanol concentration ratio was 0.85  0.24 [25]. The comparative analysis showed that the average blood/urine ethanol concentration ratio for unembalmed cadavers [25] does not differ from the values obtained for presented Case 1 and Case 3 (p = 0.669 and p = 0.286, respectively) but is higher than in presented Case 6 (p < 0.001), which most probably results from the longer time interval between the time of death and the time of the toxicological analyses.…”

Section: Discussionmentioning

confidence: 96%

Post mortem ethanol detection in corpses after embalming – preliminary study

Tomsia

Nowicka

Chełmecka

2022

Ann. Acad. Med. Siles.

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Balsamacja zwłok staje się coraz bardziej popularna ze względów sanitarnych, higienicznych lub psychologicznych. W szczególnych okolicznościach, jak transport zwłok z zagranicy, balsamowanie jest wymogiem administracyjnym. Balsamacja odbywa się za pomocą substancji chemicznych, które hamują aktywność enzymatyczną i bakteriologiczną, wstrzymując tym samym procesy rozkładu. Prezentowana praca przedstawia analizy toksykologiczne materiałów pobranych podczas sekcji sześciu zwłok poddanych procesom balsamowania oraz wyniki pomiarów stężenia użytych konserwantów w tkankach i płynach ustrojowych badanych zwłok. Analiza przeprowadzona za pomocą chromatografii gazowej z detektorem płomieniowo-jonizacyjnym (<i>gas chromatography with a flame ionization detector</i> – GC-FID) udowodniła obecność etanolu w pięciu przypadkach. Chromatografia cieczowa połączona ze spektrometrią mas (<i>liquid chromatography-mass spectrometry</i> – LC-MS) wykluczyła obecność substancji psychoaktywnych we wszystkich sześciu przypadkach. Wyniki wykazały, że w przypadku analiz toksykologicznych zabalsamowanych zwłok kluczowe znaczenie ma zabezpieczenie izolowanych płynów ustrojowych, takich jak płyn mózgowo-rdzeniowy, płyn gałki ocznej, oraz wielu materiałów referencyjnych.

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Section: Discussionmentioning

confidence: 96%

Post mortem ethanol detection in corpses after embalming – preliminary study

Tomsia

Nowicka

Chełmecka

2022

Ann. Acad. Med. Siles.

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“…Femoral blood and urine samples were collected as described in forensic guidelines [32]. Analyses of ethanol, acetone, and isopropanol levels in these samples were estimated using procedures developed by Tomsia et al [33].…”

Section: Sample Preparationmentioning

confidence: 99%

“…The carrier gas was helium (5.0 mL/min), and t-butyl alcohol was used as an internal standard. The validation procedures for isopropanol and acetone analysis in GCC and UCC were done the same way as the validation procedure for ethanol presented in the study by Tomsia et al [33].…”

Section: Volatile Substance Analysismentioning

confidence: 99%

Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies

Tomsia¹,

Nowicka²,

Skowronek³

et al. 2021

amsik

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Aim:The study aimed to examine whether volatile substances (ethanol, isopropanol, and acetone) can be detected in costal cartilage and also if concentrations of detected substances reliably reflect their concentrations in the peripheral blood -the standard forensic material for toxicological analyses. Such knowledge can be useful in cases when a cadaver's blood is unavailable or contaminated. Material and methods: Ethanol, isopropanol, and acetone concentrations were determined in samples of unground costal cartilage (UCC), ground costal cartilage (GCC), femoral venous blood, and urine. The samples were analysed by gas chromatography (GC) with a flame ionization detector using headspace analysis. Results: Volatile substances were detected in 12 out of 100 analysed samples. There was a strong positive correlation between ethanol concentration in the blood and urine (r = 0.899, p < 0.001), UCC (r = 0.809, p < 0.01), and GCC (r = 0.749, p < 0.01). A similar strong correlation was found for isopropanol concentration in the blood and urine (r = 0.979, p < 0.001), UCC (r = 0.866, p < 0.001), and GCC (r = 0.942, p < 0.001). Acetone concentration in the blood strongly correlated only with its concentration in urine (r = 0.960, p < 0.001). Conclusions: We demonstrated for the first time the possibility of detecting volatile substances: ethanol, isopropanol and acetone in a human costal cartilage. Also, the study showed that higher volatiles concentrations were better determined in ground samples.

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“…These guidelines date back to 2012 and have not been updated since, despite suggestions that they are now outdated and need to be revised [4]. It is also worth mentioning that there is a large amount of ongoing research into the potential use of alternative biological specimens in toxicological analysis, including although not limited to the use of costal cartilage in the post-mortem diagnosis of ethyl alcohol intoxication [5]. The advantages, disadvantages, and possibility of using CSF as an alternative biological specimen have been recently reviewed [6].…”

Section: Introductionmentioning

confidence: 99%

Assessing the applicability of cerebrospinal fluid collected from the spinal cord for the determination of ethyl alcohol in post-mortem toxicology

Wachholz

Skowronek

Pawlas

2022

Forensic Sci Med Pathol

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This paper presents the results of a study on the applicability of cerebrospinal fluid (CSF) collected from the spinal canal in the post-mortem determination of ethyl alcohol. The present study reviewed data of autopsy cases (n = 45), in which ethyl alcohol was detected in CSF using gas chromatography with a flame ionization detector (HS-GC-FID), to investigate ethyl alcohol concentrations in CSF, compared with blood. As a result of statistical analysis of the obtained data, a high positive correlation was found between blood ethanol concentration and cerebrospinal fluid collected from the spinal canal ethanol concentration. The Pearson correlation coefficient was statistically highly significant (p < 0.001) (r = 0.9503). The data obtained allowed us to conclude that cerebrospinal fluid collected from the spinal canal can be collected during an autopsy as an alternative biological specimen to assess the ethanol content. Cerebrospinal fluid collected from the spinal canal can corroborate and lend credibility to the results obtained for blood and, in special cases, when blood is drawn from putrefied bodies and may even be a superior specimen to blood for assessing ethyl alcohol intoxication status.

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A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

Cited by 8 publications

References 28 publications

Post mortem ethanol detection in corpses after embalming – preliminary study

Post mortem ethanol detection in corpses after embalming – preliminary study

Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies

Assessing the applicability of cerebrospinal fluid collected from the spinal cord for the determination of ethyl alcohol in post-mortem toxicology

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