A comparative study of expression of Fc receptors in relation to the autoantibody-mediated immune response and neutrophil elastase expression in autoimmune blistering dermatoses

Abstract

HereweinvestigatedthecutaneousCD32AandCD89expressioninrelationto theneutrophilelastase(NE)expressionandserumlevelofanti-desmoglein1and3 (DSG1/DSG3)IgGinpemphigus,anti-BP180/BP230IgGinbullouspemphigoid (BP),anti-gliadinnonapeptides(npG),tissue(tTG),andepidermaltransglutaminases(eTG)IgAindermatitisherpetiformis(DH). Theexaminedmaterialconsistedofskin/mucosaltissuesandsera.Intotal,87pa-tients were studied. Immunohistochemistry on paraffin-embedded sections with quantitativedigitalmorphometrywasusedtomeasuretheintensityofCD32A/ CD89/NEexpressions.Levelsofanti-DSG1/DSG3IgG,anti-BP180/BP230IgG, andanti-npG/tTG/eTGIgAwereevaluatedwithELISAs. CD32AwasabundantlyexpressedincutaneouslesionsinpemphigusandBP.We found nostatisticallysignificant correlationbetweentheCD32A/CD89 andNE expressionintensitiesinpemphigus,BP,andDH.Therewasasignificantcorrelation between CD89 expression and anti-npG IgA in DH. Our results revealed alackofcorrelationbetweenCD32Aexpressionsandanti-DSG1/DSG3IgGlevels inpemphigus,anti-BP180/BP230IgGinBPaswellasCD89expressionandan-ti-tTG/eTGIgAinDH. CD89seemstobelinkedwithglutenintoleranceinDHratherthanwithproteo-lyticdestructionofdermal-epidermaljunction.CD32Aappearstoplayanimport-antroleinmediatingskininjuryinpemphigusandBP,butprobablyindependently fromspecificautoantibodies.

Immunoexpression of IgA receptors (CD89, CD71) in dermatitis herpetiformis

“…The slides were examined by light microscopy (BX40, Olympus, Tokyo, Japan) connected to a digital camera and the images were recorded and archived. The images with positive IHC reaction were subjected to semiquantitative morphometric analysis using "HSV Filter" software originally developed in the Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, Poland [21][22][23].…”

“…We measured the area of IHC reaction and calculated the staining intensity according to the following formula: (area of positive IHC reaction/area studied) × 100% [21][22][23]. Then, the mean value was calculated for every patient and each studied groups (DH, IgAN).…”

“…Validation of IgA Fc receptors by immunohistochemistry. Immunohistochemical (IHC) staining with polyclonal rabbit antibodies against human CD89 (anti-CD89, Acris Antibodies GmbH, Herford, Germany), monoclonal murine antibodies against human CD71 (TFRC, Transferrin R antibody, Novus Biologicals, Littleton, CO, USA), monoclonal murine antibodies against human NE (clone NP57Dako, Glostrup, Denmark) and Real EnVision detection kit (Dako) were applied according to previously described procedures [19,21]. All antibodies were diluted 1:100 in Antibody Diluent (Dako).…”

“…All antibodies were diluted 1:100 in Antibody Diluent (Dako). The tests were conducted according to the classical Peroxidase-DAB staining as described in detail in our recent study [21].…”

Evaluation of a Bi-Analyte Immunoblot as a Useful Tool for Diagnosing Dermatitis Herpetiformis

“…It is postulated that IgA Fc receptors, including CD89, in DH may be related to neutrophil activation, production of autoantibodies, and gluten transport and/or transformation [27][28][29].…”