A comparative study of flow cytometric T cell receptor Vβ repertoire and T cell receptor gene rearrangement in the diagnosis of large granular lymphocytic lymphoproliferation

Abstract

FC-Vβ analysis was slightly more sensitive than TCR-GR for the detection of clonal T cell lymphoproliferation. However, we must interpret the laboratory findings with clinical context as clonal T cell lymphoproliferation may occur in patients with viral infection.

Composite lymphoma after chemotherapy with regressed diffuse large B-cell lymphoma and transformed cytotoxic T-cell lymphoma

“…In that study, they also screened 100 unselected peripheral blood samples from newly diagnosed patients with CLL and found clonal T-cell expansion in eight patients, among whom seven patients had increased lymphocytes of large granular lymphocyte (LGL) morphology. We also noted clonal LGL proliferation in a patient with underlying unclassifi able B-cell leukemia [10]. Th e cytotoxic nature of the clonal T-cell expansions in the peripheral blood correlates with the cytotoxic nature of the PTCLs, but their role in the subsequent development of PTCL is still unclear.…”

CD4 and CD8 double‐negative adult T‐cell leukemia/lymphoma with monomorphic cells expressing CD99: A diagnostic challenge in a country non‐endemic for human T‐cell leukemia virus

“…Flow cytometric study of the peripheral blood confirmed leukemic change with the same immunophenotype as the nodal specimen. Flow cytometric T‐cell receptor (TCR) Vβ repertoire analysis using a commercial kit (IOTest Beta Mark TCR‐Vβ Repertoire Kit; Beckman Coulter, Miami, Florida, USA) showed a non‐reactive pattern, indicating a clonal T‐cell lymphoproliferation according to our recent study …”

“…Flow cytometric T-cell receptor (TCR) Vb repertoire analysis using a commercial kit (IOTest Beta Mark TCR-Vb Repertoire Kit; Beckman Coulter, Miami, Florida, USA) showed a non-reactive pattern, indicating a clonal T-cell lymphoproliferation according to our recent study. 9 Subsequent serology study showed that the patient's serum was positive for anti-HTLV I/II antibody, which prompted us to perform Southern blotting analysis.…”

Diagnosis of Large Granular Lymphocytic Leukemia in a Patient Previously Treated for Acute Myeloblastic Leukemia

“…Flow cytometric T-cell receptor V repertoire analysis can also be used for diagnosis of a clonal T-cell population. 9,10 …”