A Comparative Study of HLA-DRB Typing by Transcription-Mediated Amplification with the Hybridization Protection Assay (TMA/HPA) versus PCR/SSOP

Smith, Anajane G.; sMatsubara, Koichi; Mickelson, Eric; Moheb, Ahmad; Regen, Lois; Guthrie, Leigh Ann

doi:10.1016/s0198-8859(97)00085-2

Cited by 4 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An RNA or DNA target is amplified with an amplicon, ssRNA [192,193]. The first primer anneals within the target and DNA polymerase activity of reverse transcriptase extends the DNA chain.…”

Section: Tmamentioning

confidence: 99%

A commented dictionary of techniques for genotyping

Drábek

2001

Electrophoresis

View full text Add to dashboard Cite

Several tools, differing in their technical and practical parameters, are available for the detection of point mutations as well as small deletions and insertions. In this article, a dictionary featuring over fifty methods for detection of mutation is presented. The distinguishing principle for each method is briefly explained. Sorting of and discussion on the methods give the reader a brief introduction to the field of genotyping.

show abstract

“…An RNA or DNA target is amplified with an amplicon, ssRNA [192,193]. The first primer anneals within the target and DNA polymerase activity of reverse transcriptase extends the DNA chain.…”

Section: Tmamentioning

confidence: 99%

A commented dictionary of techniques for genotyping

Drábek

2001

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…In 1995, UNOS mandated the sharing of all HLA-0MM kidneys, a decision which allowed a “blank” (e.g., A2-, B7-, and DR4-) to be considered as evidence of homozygosity for the purposes of the sharing program. This new confidence was based on significant advances in accuracy of HLA typing made possible by the polymerase chain reaction technique with sequence-specific primer (5) and other DNA-based approaches (9). …”

mentioning

confidence: 99%

HLA-A, -B, and -DR Zero-Mismatched Kidneys Shipped to the University of Wisconsin, Madison, 1993–2006: Superior Graft Survival Despite Longer Preservation Time

Burlingham

Río²,

Lorentzen³

et al. 2010

Transplantation

View full text Add to dashboard Cite

Background To determine the impact at a single center of the United Network for Organ Sharing-mandated sharing program for human leukocyte antigen (HLA)-A/-B/-DR 0-mismatched (0MM) kidneys, we analyzed the results of 264 kidney transplants from 0MM distant donors between 1993 and 2006, with a follow-up through January 31, 2007. We compared these results with that of concurrent kidneys transplanted from HLA more than 0MM local donors and with shipped more than 0MM kidneys from “payback” donors. Results Despite a significantly longer preservation time, we found an 11% increase in 8-year graft survival (63% vs. 52% P<0.003) of 0MM shipped versus locally procured, >0MM donor kidneys. Graft survival of 0MM shipped kidneys at 8 years was significantly better in nonsensitized (<20% panel reactive antibodies; 68% vs. 55% P<0.0005) but not in sensitized (≥20% panel reactive antibodies) recipients, who showed an early (2 years) but short-lived benefit. The benefit of receiving a HLA-A, -B, and -DR 0MM shipped kidney remained strong and statistically significant (0.71 relative risk of graft loss vs. local; P<0.02) when adjusted for 22 potentially confounding variables in a Cox proportional hazards analysis. Conclusions The recent change in United Network for Organ Sharing policy restricting mandated sharing of 0MM kidneys to sensitized and pediatric recipients will give greater flexibility to the local organ procurement organization in allocating organs. However, the survival benefit to nonsensitized patients is real and long lasting and will be lost.

show abstract

“…However, DNA typing techniques are not free of errors because they depend on sensitive procedures conducted at specific temperatures, good DNA quality, adequate reagents, and also correct interpretation of the results. Despite the importance of the reliability of different HLA DNA typing methods, few comparative studies have been performed between these techniques (Smith et al 1997).…”

mentioning

confidence: 99%