A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass

K, Madhumitha; Anbumalarmathi, J.; Sharmili, S. Aruna; Nandhini, G.; Priya, G. Shanmuga

doi:10.9734/arrb/2020/v35i330195

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Identification of specific components was done by comparing mass spectra and the retention times with the data provided with the NIST library and confirmed. The peak area percentages of the chromatography represent the abundance of the compounds in the extract [9]. The peak area percentage was calculated by the formula,…”

Section: Gas Chromatography -Mass Spectrometry (Gc-ms) Analysismentioning

confidence: 99%

A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

Sireen¹,

Anbumalarmathi²

2020

IJBcRR

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Abutilon indicum is consider to be used in the traditional system of medicine. It is found in tropical and subtropical regions of the world. It is used to treat various diseases. This plant does not cause any side effects to humans. As the plant has wide variety of medicinal properties, the present study aimed to comparative between plant and callus extract of Abutilon indicum (L.) sweet for antioxidant, antibacterial, antidiabetic and anti- proliferative activity. The highest percentage of callus induction (89.50%) and callus weight (1.26 g) was observed in T5 (MS + 2, 4-D (2.5 mg/l) + BAP (2 mg/l) and T8 [IBA (4 mg/l)] respectively. Phytochemical analysis of aqueous and ethyl acetate extracts of A. indicum in vivo plant and in vitro grown callus showed the presence of alkaloids, flavonoids, phenols, carbohydrates, glycosides, protein, terpenoids, saponins, tannins and coumarin. The total phenolic content was high in aqueous extract of callus (30.68 mg TAE/g). Maximum DPPH radical scavenging activity was found in aqueous extract of callus (86%) with IC50 value of 68.49 µg/ml. FT-IR analysis of aqueous extract of A. indicum plant and callus showed the presence of characteristic stretching at 2930.28 and 2927.75 indicating the presence of C-H stretching respectively. GC-MS analysis revealed the presence of 17 compounds in ethyl acetate plant extract, whereas 7 compounds in ethyl acetate callus extract such as tetradecane, 1-chloro, Sulfurous acid 2-prophytridecyl ester and 1- ethyl-3-[2-(octadecylthio) ethyl] thiourea. The ethyl acetate extracts of callus and plant and was found to be effective against Bacillus subtilis (3.1 mm) and Staphylococcus aureus (2.9 mm). Maximum α-amylase inhibitory activity was observed in aqueous callus extract (32.65%) with IC50 value of 833.61 µg/ml. HeLa cell viability was found to be 26.8% and 21.8% in plant and callus extract respectively.

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Section: Gas Chromatography -Mass Spectrometry (Gc-ms) Analysismentioning

confidence: 99%

A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

Sireen¹,

Anbumalarmathi²

2020

IJBcRR

Self Cite

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“…It is a fragrant, bushy, perennial plant and attains a height up to 45-60 cm, well-branched with purple flowers at the terminal ends, with aromatic leaves [4]. It is commonly known as Lark daisy, Kesavardhini, and Brazilian Bachelor's Button [4,5]. It is known for its medicinal value and is used to treat various ailments.…”

Section: Introductionmentioning

confidence: 99%

“…It is known for its medicinal value and is used to treat various ailments. It was highlighted that C. punctatum (being rich in secondary metabolites) serves as a health-caring plant with various beneficial properties including antimicrobial, anti-oxidant, anti-allergic, anti-inflammatory, and cytotoxic anti-tumor effects [5]. Due to these important facts, the plant is referred to as a traditional wound healer.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Propagation and Phytochemical Composition of Centratherum punctatum Cass—A Medicinal Plant

Talan,

Mujib,

Ejaz

et al. 2023

Horticulturae

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An effective and reproducible micropropagation protocol was developed for Centratherum punctatum Cass. Successful in vitro initiation of callus and subsequent plant regeneration were obtained on nodal explants cultured on MS medium supplemented with plant growth regulators (PGRs). The maximum frequency of callus formation (98.3%) was noted on MS containing 4.0 mg/L 6-Benzylaminopurine (BAP) and 3.5 mg/L Kinetin with a maximum callus weight of 2.02 g. The best shoot induction frequency (100%) with an average of 30.2 shoots per explant was achieved when 4.5 mg/L BAP and 4.0 mg/L Kinetin were added to the MS. The same PGR combination resulted in the best callus-mediated shoot formation (8.3 shoots/callus mass). The highest rhizogenic response (95.3%) with an average 26.1 roots per shoot and root length of 6.2 cm was obtained with 1.0 mg/L Indole-3-acetic acid (IAA)-supplemented MS medium. The gas chromatography–mass spectrometry (GC-MS) technique was applied in the present study to analyze the methanolic extracts of the leaf, callus, and root of regenerated C. punctatum shoots to detect the different phytochemical constituents. The leaf extract of the regenerated C. punctatum showed 37 phytocompounds; some important bioactive compounds were the Phytol,1,6-Octadien 3,5-Dimethyl-Cis, 4,8-Dimethylnona-3,8-dien-2-one, 2,6-Octadiene, Stigmasterol, Chondrillasterol, Lanosteryl acetate, etc. In the callus, the extract had a total of 57 phytocompounds; among them, the Stigmasterol, Guanosine, and Tri-decanoic acid were the major ones. In the root extract, the GC-MS revealed a low number of 23 phytocompounds, the important compounds of which were Stigmasterol, Trimethylsilyl (TMS) derivative, Chrysantenyl 2-methuylbutanoate, 4-tert-Butoxybutan-1-ol, etc. The order in terms of numbers of phytocompounds present in tissue sources are callus > leaf > root.

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Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

Aswathi

Thomas

2023

In Vitro Cell.Dev.Biol.-Plant

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Centratherum punctatum Cass. is an important medicinal herb used for various purposes. The present study describes an e cient protocol for the micropropagation of C. punctatum through transverse Thin Cell Layers (tTCLs). The effect of plant growth regulators (PGRs), thickness of tTCLs and source of tTCLs, were evaluated. The tTCLs of varying thickness (0.5-5.0 mm) were excised from 10-day-old leaf, 45-day-old node and internode and cultured on MS medium supplemented with various concentrations of PGRs (BAP, TDZ, KN; 0.1-2.0 mg/l) alone or in combination with NAA (0.1-1.0 mg/l) for shoot induction.For callus induction 2, 4-D (0.5-2.0 mg/l) was employed. The leaf and node tTCLs showed direct shoot regeneration and internodal tTCLs resulted in calli which successfully regenerated to shoots on subculture. A signi cant effect of thickness/width on shoot induction was observed. For leaf, 1.0 mm width and for node and internode 2.0 mm thick tTCLs showed maximum response. MS medium supplemented with 1.5 mg/l TDZ, 0.5 mg/l TDZ and 1.0 mg/l BAP in combination with 0.2 mg/l NAA were found optimum for shoot induction from tTCLs of leaf (96% response with 41.5 shoots per explant), internode (64% response with 22.9 shoots per explant) and node (87% response with 11.9 shoots per explant) respectively. Rhizogenesis was obtained when micropropagated shoots were transferred to half strength MS medium supplemented with various concentrations (0.25-2.0 mg/l) of IBA. The rooted plantlets were eventually acclimatized and transferred to soil. The procedure described here is a promising tool for micropropagation of C. punctatum as it produces high frequency healthy shoots from minimum explant source. Key MessageA rapid and e cient micropropagation protocol has been standardized in C. punctatum through transverse thin cell layer (tTCL) explants from leaf, node and internode.

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A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass

Cited by 4 publications

References 5 publications

A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

In Vitro Propagation and Phytochemical Composition of Centratherum punctatum Cass—A Medicinal Plant

Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

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