A comparative study of leukaemia inhibitory factor and interleukin-1α intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate

Parodi, Alessandro; Sanguineti, Roberta; Catalano, Mariafrancesca; Penco, Susanna; Pronzato, Maria Adelaide; Scanarotti, Chiara; Bassi, Anna Maria

doi:10.1016/j.toxlet.2009.10.013

Cited by 12 publications

(12 citation statements)

References 41 publications

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“…The inflammatory response includes the recruitment of leukocytes and release of inflammatory cytokine, such as TNF- α , IL-1, IL-6, IL-10, and others [40, 41]. Various constituents of essential oils effectively inhibit cytokine production.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of Anti-Inflammatory Activity ofCitrus latifoliaTanaka Essential Oil and Limonene in Experimental Mouse Models

Kummer

Fachini-Queiroz

Estevão-Silva

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The genus Citrus (Rutaceae) includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity of Citrus latifolia Tanaka essential oil (CLEO) and its main constituent LIM. In the cell viability assay, CLEO and LIM (3, 10, 30, and 90 μg/mL) had low cytotoxicity. In zymosan-induced peritonitis, LIM (500 mg/kg) decreased the infiltration of peritoneal exudate leukocytes and decreased the number of polymorphonuclear leukocytes. In vitro chemotaxis revealed that CLEO and LIM (1, 3, and 10 µg/mL) promoted a significant reduction of neutrophil migration toward fMLP and LTB4. LIM (500 mg/kg) also reduced TNF-α levels but did not alter IL-10 levels in the peritoneal exudate. In conclusion, this study showed that LIM isolated from CLEO had potential anti-inflammatory effects, likely by inhibiting proinflammatory mediators present in inflammatory exudate and leukocyte chemotaxis.

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Section: Resultsmentioning

confidence: 99%

“…Citrus essential oil itself did not reduce IL-10 levels [45]. LIM exerts anti-inflammatory activity by reducing PGE 2 production in macrophages [19] and IL-1 α levels in normal human undifferentiated NCTC 2544 keratinocytes [40]. Other compounds present in CLEO also have some anti-inflammatory effects.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Anti-Inflammatory Activity ofCitrus latifoliaTanaka Essential Oil and Limonene in Experimental Mouse Models

Kummer

Fachini-Queiroz

Estevão-Silva

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…During the inflammatory event there is also an increase in cytokine production. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interferon-γ (IFN-γ) have important roles in the maintenance of the inflammatory profile [ 26 , 27 ]. Our results indicate that EOs have significant anti-inflammatory effects in reducing all parameters evaluated.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species

et al. 2016

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Citrus fruits have potential health-promoting properties and their essential oils have long been used in several applications. Due to biological effects described to some citrus species in this study our objectives were to analyze and compare the phytochemical composition and evaluate the anti-inflammatory effect of essential oils (EO) obtained from four different Citrus species. Mice were treated with EO obtained from C. limon, C. latifolia, C. aurantifolia or C. limonia (10 to 100 mg/kg, p.o.) and their anti-inflammatory effects were evaluated in chemical induced inflammation (formalin-induced licking response) and carrageenan-induced inflammation in the subcutaneous air pouch model. A possible antinociceptive effect was evaluated in the hot plate model. Phytochemical analyses indicated the presence of geranial, limonene, γ-terpinene and others. EOs from C. limon, C. aurantifolia and C. limonia exhibited anti-inflammatory effects by reducing cell migration, cytokine production and protein extravasation induced by carrageenan. These effects were also obtained with similar amounts of pure limonene. It was also observed that C. aurantifolia induced myelotoxicity in mice. Anti-inflammatory effect of C. limon and C. limonia is probably due to their large quantities of limonene, while the myelotoxicity observed with C. aurantifolia is most likely due to the high concentration of citral. Our results indicate that these EOs from C. limon, C. aurantifolia and C. limonia have a significant anti-inflammatory effect; however, care should be taken with C. aurantifolia.

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“…Therefore, single cell assay and cytotoxicity assay have been employed in many studies to predict the skin irritation potential of chemicals. For example, human keratinocyte (HaCaT, NCTC 2544) monolayer cultures have been used as an in vitro model to predict potential skin irritation with cytotoxicity as endpoints [39, 52–54]. Therefore in our study, we also used single cell assay with cytotoxicity to evaluate the skin irritation potential of DHNA and dithranol.…”

Section: Discussionmentioning

confidence: 99%

“…Only followed cell injury, IL-1 α is exclusively released from leaky keratinocytes while in intact epidermis, IL-1 α is naturally eliminated by desquamation [37]. In both in vitro and in vivo systems the release of IL-1 α is accepted as an early marker of irritation [39, 54]. Therefore, together with cell viability test, measuring the distribution of cytokines (release of IL-1 α ) is an effective and promising approach to predict chemicals as potential irritants [39].…”

Section: Discussionmentioning

confidence: 99%

1,4-dihydroxy-2-naphthoic Acid Induces Apoptosis in Human Keratinocyte: Potential Application for Psoriasis Treatment

Mok

Xie

Sham

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Psoriasis, which affects approximately 1–3% of the population worldwide, is a chronic inflammatory skin disorder characterized by epidermal keratinocytes hyperproliferation, abnormal differentiation, and inflammatory infiltration. Decrease in keratinocyte apoptosis is a specific pathogenic phenomenon in psoriasis. Chinese herbs have been used for the treatment of psoriasis in China showing promising effect in clinical trials. A traditional Chinese medicine has relatively fewer side effects with longer remission time and lower recurrence rate. The extract of Rubia cordifolia L. (EA) was previously found by us to induce HaCaT keratinocytes apoptosis. In this study we identified one of the components in Rubia cordifolia L., the anthraquinone precursor 1,4-dihydroxy-2-naphthoic acid (DHNA), induces HaCaT keratinocytes apoptosis through G0/G1 cell cycle arrest. We have also demonstrated that DHNA acts through both caspase-dependent and caspase-independent pathways. Besides, cytotoxicity and IL-1α release assays indicate that DHNA causes less irritation problems than dithranol, which is commonly employed to treat psoriasis in many countries. Since DHNA possesses similar apoptotic effects on keratinocytes as dithranol but causes less irritation, DHNA therefore constitutes a promising alternative agent for treating psoriasis. Our studies also provide an insight on the potential of using EA and DHNA, alternatively, as a safe and effective treatment modality for psoriasis.

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A comparative study of leukaemia inhibitory factor and interleukin-1α intracellular content in a human keratinocyte cell line after exposure to cosmetic fragrances and sodium dodecyl sulphate

Cited by 12 publications

References 41 publications

Evaluation of Anti-Inflammatory Activity ofCitrus latifoliaTanaka Essential Oil and Limonene in Experimental Mouse Models

Evaluation of Anti-Inflammatory Activity ofCitrus latifoliaTanaka Essential Oil and Limonene in Experimental Mouse Models

Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species

1,4-dihydroxy-2-naphthoic Acid Induces Apoptosis in Human Keratinocyte: Potential Application for Psoriasis Treatment

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