1985
DOI: 10.1007/bf02450473
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A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues

Abstract: Around the turn of the century, tonofibrils and contractile myofibrils were observed within the same cells. These findings have been largely forgotten. To clarify the topical relations of these proteins in epithelial cells, duplicate sections of methacarn-fixed human and canine tissues were treated with the tannic acid-phosphomolybdic acid (TP)-Levanol Fast Cyanine 5RN reaction for myosins and the PAP technic for prekeratin, respectively. In bronchi, lingual and sweat glands, liver and pancreas, myosin was con… Show more

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Cited by 6 publications
(3 citation statements)
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“…Images of Congo red stained plaques were obtained on the Zeiss Axiophot microscope at 403 magnification, both in brightfield mode and in fluorescence using a FITC filter set (absorption maximum, 495 nm; emission maximum, 525 nm). The red fluorescence under UV light illumination 32 was more sensitive than examination under visible light for detection of very small Congo red stained amyloid deposits. 33 Plaque dimensions in Congo red (as well as in immunostained sections) were measured with MacProbe v4.4 software, following calibration with a stage micrometer.…”
Section: Congo Red Stainingmentioning
confidence: 99%
“…Images of Congo red stained plaques were obtained on the Zeiss Axiophot microscope at 403 magnification, both in brightfield mode and in fluorescence using a FITC filter set (absorption maximum, 495 nm; emission maximum, 525 nm). The red fluorescence under UV light illumination 32 was more sensitive than examination under visible light for detection of very small Congo red stained amyloid deposits. 33 Plaque dimensions in Congo red (as well as in immunostained sections) were measured with MacProbe v4.4 software, following calibration with a stage micrometer.…”
Section: Congo Red Stainingmentioning
confidence: 99%
“…This structure can be selectively recognized by thiazole dyes, such as thioflavin T and S, good indicators of amyloid-like conformation (38,39). To obtain conformational information, we double-stained rotenone-treated cells with an anti-␣-syn antibody and thioflavin S. As described above, two forms of aggregates can be detected after rotenone treatment, small cytoplasmic aggregates and large perinuclear inclusions.…”
Section: ␣-Synuclein Aggregation By Mitochondrial Inhibitorsmentioning
confidence: 99%
“…Methacarn was prepared according to Puchtler et al 15 by mixing 60% absolute methanol (vol/vol), 30% chloroform, and 10% glacial acetic acid. After fixation and routine processing, each individual lymph node was embedded in paraffin and serially sectioned as follows: (a) 1 section stained with H&E (3 μm thick), (b) 3 sections (3 μm thick) collected on positively charged slides for IHC staining, (c) 6 sections (7 μm thick) collected in ribonuclease (RNase)and deoxyribonuclease (DNase)-free tubes for molecular investigation, and (d) 1 section treated with H&E (control).…”
Section: Surgical Specimensmentioning
confidence: 99%