A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and β-TCP ceramics

Liu, Qihai; Chen, Lian; Yin, Shuo; Chen, Lei; Liu, Guangpeng; Chang, Jiang; Cui, Lei

doi:10.1016/j.biomaterials.2008.08.039

Cited by 226 publications

(189 citation statements)

References 37 publications

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“…38,39 Compared with β-TCP (Ca 3 PO 4 , representative of bioceramics), AKT not only promoted cell attachment, proliferation, osteogenic differentiation and angiogenesis in vitro but also enhanced in vivo osteogenesis and angiogenesis. 31,32,40 Therefore, AKT was employed as the model material for growing MoS 2 nanosheets to fabricate MS-AKT scaffolds, and the osteogenic potential of these scaffolds was evaluated in the present study. The in vitro experiments demonstrated that both MS-AKT and AKT scaffolds supported the attachment and proliferation of rBMSCs, and even low concentrations of MS-AKT extracts significantly enhanced the proliferation of rBMSCs compared to AKT groups without MoS 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Akermanite (AKT, Ca 2 MgSi 2 O 7 ), a Ca-, Mg-and Si-containing bioceramic, was selected as the matrix material for carrying MoS 2 nanosheets because it readily promotes osteogenesis and angiogenesis, as shown in our previous study. 31,32 The structure and composition of the MoS 2 -modified AKT (MS-AKT) scaffolds were characterized by optical microscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The photothermal performance of the scaffolds was then systematically investigated by altering the scaffold sizes, laser power densities and MoS 2 content.…”

Section: Introductionmentioning

confidence: 99%

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A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration

Wang

et al. 2017

NPG Asia Mater

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140

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The treatment of malignant bone tumors is a significant clinical challenge because it requires the simultaneous removal of tumor tissues and regeneration of bone defects, and bifunctional three-dimensional (3D) scaffolds that function in both tumor therapy and tissue regeneration are expected to address this need. In this study, novel bifunctional scaffolds (MS-AKT scaffolds) were successfully fabricated by combining a 3D printing technique with a hydrothermal method. During the hydrothermal process, MoS 2 nanosheets were grown in situ on the strut surface of bioceramic scaffolds, endowing them with photothermal therapeutic potential. Under near-infrared (NIR) irradiation, the temperature of the MS-AKT scaffolds rapidly increased and was effectively modulated by varying the MoS 2 content, scaffold sizes and laser power densities. The photothermal temperature significantly decreased the viability of osteosarcoma cells and breast cancer cells and inhibited tumor growth in vivo. Moreover, the MS-AKT scaffolds supported the attachment, proliferation and osteogenic differentiation of bone mesenchymal stem cells and induced bone regeneration in vivo. This bifunctional scaffold, which treats the tumor and facilitates bone growth, offers a promising clinical strategy to treat tumor-induced bone defects. This proof-of-concept study demonstrates the feasibility of localized tumor therapy and tissue regeneration in diverse tissue engineering applications using multifunctional biomaterials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration

Wang

et al. 2017

NPG Asia Mater

Self Cite

140

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“…Composite materials attempt to take advantage of the strengths of multiple materials. For instance, a gel can be used as a "cell carrier", filling large holes in a rigid scaffold, and providing a soft support for cells in vulnerable first stages, but maintaining a large space for bone formation in later stages (Hao et al 2010;Liu et al 2008). The growth surfaces of neighboring cells may influence ASC osteogenesis indirectly through paracrine signaling (Lu et al 2011).…”

Section: Substrate Stimulusmentioning

confidence: 99%

“…NO and Cox-2 are reported to be involved in bone mechanical adaptation. NO and integrin α5β1 participate in ERK 1/2 activation scaffold (Liu et al 2008). This group assessed osteogenic capacity by measuring osteocalcin deposition and by realtime PCR analysis for expression of osteogenic marker genes alkaline phosphatase (ALP) and osteocalcin (OCN).…”

Section: Substrate Stimulusmentioning

confidence: 99%

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

Salazar

Onodera

2012

Biophys Rev

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Developing bone is subject to the control of a broad variety of influences in vivo. For bone repair applications, in vitro osteogenic assays are routinely used to test the responses of bone-forming cells to drugs, hormones, and biomaterials. Results of these assays are used to predict the behavior of bone-forming cells in vivo. Stem cell research has shown promise for enhancing bone repair. In vitro osteogenic assays to test the bone-forming response of stem cells typically use chemical solutions. Stem cell in vitro osteogenic assays often neglect important biophysical cues, such as the forces associated with regular weight-bearing exercise, which promote bone formation. Incorporating more biophysical cues that promote bone formation would improve in vitro osteogenic assays for stem cells. Improved in vitro osteogenic stimulation opens opportunities for "preconditioning" cells to differentiate towards the desired lineage. In this review, we explore the role of select biophysical factors-growth surfaces, tensile strain, fluid flow and electromagnetic stimulation-in promoting osteogenic differentiation of stem cells from human adipose. Emphasis is placed on the potential for physical microenvironment manipulation to translate tissue engineering and stem cell research into widespread clinical usage.

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“…Meanwhile, the surface chemistry and topography of materials play a very crucial role in altering cell behaviors at many stages of cell growth and development. [[qv: 1c]],3 Although the dimensions of mammalian cells are in the order of a few micrometers, cellular sensing of the external environment and interaction with biomaterials occurs at the nanometer level 4. Cell interactions with nanometric surfaces often result in a specific sequence of gene and protein regulations.…”

Section: Introductionmentioning

confidence: 99%

Virus Nanoparticles Mediated Osteogenic Differentiation of Bone Derived Mesenchymal Stem Cells

et al. 2015

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There are few methodologies that allow manipulating a biomaterial surface at nanometer scale, which controllably influence different cellular functions. In this study, virus nanoparticles with different structural features are selected to prepare 2D substrates with defined nanoscale topographies and the cellular responses are investigated. It is demonstrated that the viral nanoparticle based substrates could accelerate and enhance osteogenesis of bone derived mesenchymal stem cells as indicated by the upregulation of osteogenic markers, including bone morphogenetic protein‐2, osteocalcin, and osteopontin, at both gene and protein expression levels. Moreover, alkaline phosphatase activity and calcium mineralization, both indicators for a successful bone formation, are also increased in cells grown on these nanoscale possessed substrates. These discoveries and developments present a new paradigm for nanoscale engineering of a biomaterial surface.

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A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and β-TCP ceramics

Cited by 226 publications

References 37 publications

A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration

A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

Virus Nanoparticles Mediated Osteogenic Differentiation of Bone Derived Mesenchymal Stem Cells

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