A Comparative Study of Rutin and Rutin Glycoside: Antioxidant Activity, Anti-Inflammatory Effect, Effect on Platelet Aggregation and Blood Coagulation

Choi, Sung-Sook; Park, Hye-Ryung; Lee, Kyung-Ae

doi:10.3390/antiox10111696

Cited by 67 publications

(27 citation statements)

References 49 publications

(49 reference statements)

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“…Our study showed that the incidence of postoperative lower limb swelling and deep vein in the study group was lower than that in the control group ( P < 0.05). IL-6 and TNF- α are important inflammatory factors that mediate the process of platelet aggregation [ 17 ]. Some scholars believe that inflammatory factors may play key roles in the occurrence and development of LEDVT [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Influences of Antithrombotic Elastic Socks Combined with Air Pressure in Reducing Lower Extremity Deep Venous Thrombosis for Patients Undergoing Cardiothoracic Surgery

Zhang

Sun

et al. 2022

Computational and Mathematical Methods in Medicine

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This study was designed to investigate the application and therapeutic effect of antithrombotic elastic socks combined with air pressure in the prevention of lower extremity deep venous thrombosis in patients undergoing cardiothoracic surgery. Sixty patients in cardiothoracic surgery of our hospital from January 2019 to December 2020 were randomly divided into a study group and control group. The control group was treated with routine treatment intervention. Based on routine treatment intervention, the study group was treated with antithrombotic elastic socks combined with pneumatic treatment intervention. The activated partial thromboplastin time (APTT), thrombin time (TT), femoral venous blood flow velocity of both lower limbs, and the incidence of lower extremity deep venous thrombosis (LEDVT), postoperative lower extremity swelling, inflammatory factors, and satisfaction were measured. After intervention, APTT ( 31.74 ± 1.15 s) and TT ( 14.58 ± 0.24 s) in the study group were higher than those in the control group APTT ( 25.13 ± 1.14 s) and TT ( 12.14 ± 0.23 s) ( P < 0.05 ). The left lower limb femoral vein blood flow velocity and the right lower limb femoral vein blood flow velocity in the study group were better than those in the control group ( P < 0.05 ). The incidence of postoperative lower limb swelling and deep vein in the study group was lower than that in the control group ( P < 0.05 ). Serum tumor necrosis factor alpha and interleukin-6 concentrations in the study group were lower than those in the control group ( P < 0.05 ). The satisfaction rate of patients in the study group (93.33%) was significantly higher than that in the control group (70.00%) ( P < 0.05 ). In conclusion, after cardiothoracic surgery, antithrombotic elastic socks combined with air pressure can significantly reduce the incidence of LEDVT by improving patients’ coagulation function, reducing inflammatory reaction. It is worthy of popularization and application in relevant surgery.

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Section: Discussionmentioning

confidence: 99%

Influences of Antithrombotic Elastic Socks Combined with Air Pressure in Reducing Lower Extremity Deep Venous Thrombosis for Patients Undergoing Cardiothoracic Surgery

Zhang

Sun

et al. 2022

Computational and Mathematical Methods in Medicine

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“…According to our data, RUT showed a slight toxicity against HepaRG cells at the highest concentrations used (50 and 100 µM), whereas the H9c2(2-1) and HaCaT cells were not affected (Figure 3), what indicates a cell-type and concentration-dependent cytotoxic effect. These data are supported by other studies, as follows: 1) no significant impact was induced by RUT in H9c2(2-1) cells (up to 100 μM, after 24 h of treatment) or hepatocytes (up to 500 μg/ ml, after 24 and 48 h of treatment) (Jeong et al, 2009;El-Maadawy et al, 2021); 2) a low cytotoxicity was described in RAW 264.7 macrophages and Vero kidney epithelial cells after 24 h treatment (Mecenas et al, 2018); 3) a significant reduction of RAW 264.7 cells' viability starting with 100 µM (Choi et al, 2021); 4) an inhibitory effect on HaCaT cells' growth (IC 50 = 60 μg/ml) (Deepika et al, 2019); 5) a significant decrease in cell viability of Vero normal kidney cells at 100 and 250 µM after a 48 h treatment (Caparica et al, 2020), and 6) a reduced cell viability of human dermal fibroblasts (HDFs) starting with 50 µM after 24 h exposure (Choi et al, 2016). Moreover, in our previous study concentrations of RUT as high as 75 µM induced a proliferative effect in HaCaT cells (Pinzaru et al, 2021).…”

Section: Discussionmentioning

confidence: 97%

Rutin bioconjugates as potential nutraceutical prodrugs: An in vitro and in ovo toxicological screening

et al. 2022

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Rutin (RUT) is considered one the most attractive flavonoids from a therapeutic perspective due to its multispectral pharmacological activities including antiradical, anti-inflammatory, antiproliferative, and antimetastatic among others. Still, this compound presents a low bioavailability what narrows its clinical applications. To overcome this inconvenience, the current paper was focused on the synthesis, characterization, and toxicological assessment of two RUT bioconjugates obtained by enzymatic esterification with oleic acid (OA) and linoleic acid (LA)—rutin oleate (RUT-O) and rutin linoleate (RUT-L), as flavonoid precursors with improved physicochemical and biological properties. Following the enzymatic synthesis in the presence of Novozyme® 435, the two bioconjugates were obtained, their formation being confirmed by RAMAN and FT-IR spectroscopy. The in vitro and in ovo toxicological assessment of RUT bioconjugates (1–100 µM) was performed using 2D consecrated cell lines (cardiomyoblasts - H9c2(2-1), hepatocytes—HepaRG, and keratinocytes—HaCaT), 3D reconstructed human epidermis tissue (EpiDerm™), and chick chorioallantoic membranes, respectively. The results obtained were test compound, concentration—and cell-type dependent, as follows: RUT-O reduced the viability of H9c2(2-1), HepaRG, and HaCaT cells at 100 µM (to 77.53%, 83.17%, and 78.32%, respectively), and induced cell rounding and floating, as well as apoptotic-like features in the nuclei of all cell lines, whereas RUT-L exerted no signs of cytotoxicity in all cell lines in terms of cell viability, morphology, and nuclear integrity. Both RUT esters impaired the migration of HepaRG cells (at 25 µM) and lack irritative potential (at 100 µM) in vitro (tissue viability >50%) and in ovo (irritation scores of 0.70 for RUT-O, and 0.49 for RUT-L, respectively). Computational predictions revealed an increased lipophilicity, and reduced solubility, drug-likeness and drug score of RUT-O and RUT-L compared to their parent compounds—RUT, OA, and LA. In conclusion, we report a favorable toxicological profile for RUT-L, while RUT-O is dosage-limited since at high concentrations were noticed cytotoxic effects.

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“…Enzymatic hydrolysis and transglycosylation were performed based on the methods described in Baik et al [ 23 ] and Choi et al [ 24 ] with some modification. For the preparation of hesperetin, hesperidin powder was dissolved in 0.1 N NaOH solution at a concentration of 1% ( w / v ).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of Hesperetin, Hesperidin and Hesperidin Glucoside: Antioxidant, Anti-Inflammatory, and Antibacterial Activities In Vitro

Choi

Lee

2022

Antioxidants

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The antioxidant, anti-inflammatory and antibacterial activities of hesperetin, hesperidin and hesperidin glucoside with different solubility were compared in vitro. Hesperetin was prepared by enzymatic hydrolysis from hesperidin, and hesperidin glucoside composed of hesperidin mono-glucoside was prepared from hesperidin through enzymatic transglycosylation. Solubility of the compounds was different: the partition coefficient (log P) was 2.85 ± 0.02 for hesperetin, 2.01 ± 0.02 for hesperidin, and −3.04 ± 0.03 for hesperidin glucoside. Hesperetin showed a higher effect than hesperidin and hesperidin glucoside on radical scavenging activity in antioxidant assays, while hesperidin and hesperidin glucoside showed similar activity. Cytotoxicity was low in the order of hesperidin glucoside, hesperidin, and hesperetin in murine macrophage RAW264.7 cells. Treatment of the cells with each compound reduced the levels of inflammatory mediators, nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). Hesperetin was most effective at relatively low concentrations, however, hesperidin glucoside was also effective at higher concentration. Hesperetin showed higher antibacterial activity than hesperidin in both Gram-positive and -negative bacteria, and hesperidin glucoside showed similarly higher activity with hesperetin depending on the bacterial strain. In conclusion, hesperetin in the form of aglycone showed more potent biological activity than hesperidin and hesperidin glucoside. However, hesperidin glucoside, the highly soluble form, has been shown to increase the activity compared to poorly soluble hesperidin.

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A Comparative Study of Rutin and Rutin Glycoside: Antioxidant Activity, Anti-Inflammatory Effect, Effect on Platelet Aggregation and Blood Coagulation

Cited by 67 publications

References 49 publications

Influences of Antithrombotic Elastic Socks Combined with Air Pressure in Reducing Lower Extremity Deep Venous Thrombosis for Patients Undergoing Cardiothoracic Surgery

Influences of Antithrombotic Elastic Socks Combined with Air Pressure in Reducing Lower Extremity Deep Venous Thrombosis for Patients Undergoing Cardiothoracic Surgery

Rutin bioconjugates as potential nutraceutical prodrugs: An in vitro and in ovo toxicological screening

A Comparative Study of Hesperetin, Hesperidin and Hesperidin Glucoside: Antioxidant, Anti-Inflammatory, and Antibacterial Activities In Vitro

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