A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

Serrano, M. C. López; Ramírez, M.; Morilla, D.; Valverde, Anastasio; Diego, María José Chávez de; Espinel-Ingroff, Ana; Claro, R.; Fernandez, AF; Almeida, C.; Martín-Mazuelos, Estrella

doi:10.1093/jac/dkh172

Cited by 64 publications

(37 citation statements)

References 30 publications

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“…The correlation results with four of the five Neo-Sensitabs tablets in this study (R, 0.72 to 0.92; R 2 , 0.518 to 0.846) also were comparable to those reported for Aspergillus spp. (13,16,21) and zygomycete (13,16) isolates with voriconazole (R, 0.79) and posaconazole (R, 0.72 to 0.82) disks as well as in the prior collaborative disk study (R, 0.71 to 0.91) (14).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Neo-Sensitabs Tablet Diffusion Assay with CLSI Broth Microdilution M38-A and Disk Diffusion Methods for Testing Susceptibility of Filamentous Fungi with Amphotericin B, Caspofungin, Itraconazole, Posaconazole, and Voriconazole

Espinel-Ingroff

Cantón

2008

J Clin Microbiol

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We compared the Neo-Sensitabs tablet assay to both reference M38-A broth microdilution and disk diffusion methods for testing the susceptibility of 183 filamentous isolates to amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole. Neo-Sensitabs and disk assay inhibition zone diameters, in millimeters, were obtained on nonsupplemented Mueller-Hinton agar at 16 to 48 h. The reproducibility of zone diameters (i.e., the percentage of replicate zone diameters that were within 2 standard deviations of the means), their correlation with either MICs or minimum effective concentrations (for caspofungin only), and the categorical agreement were similar between tablet and disk assays (93 to 100% [R, >0.70] and 79 to 96%, respectively) with four of the five agents. The exceptions were the results for posaconazole tablets (R, 0.686; disk, 0.757; 84% categorical agreement for tablet and 96% for disk). These data suggest the potential value of the Neo-Sensitabs assay for testing 5-g caspofungin and 1-g voriconazole posaconazole tablets against all mold isolates, 8-g itraconazole and 5-g tablets against all mold isolates except zygomycetes, and 10-g amphotericin B tablets against zygomycete isolates only.The Clinical and Laboratory Standards Institute (CLSI; formerly NCCLS) Subcommittee on Antifungal Susceptibility Tests has developed a reproducible procedure for antifungal susceptibility testing of filamentous fungi (molds) by a broth microdilution format (4, 9, 10). More recently, an agar diffusion method has been developed for testing molds by disk diffusion methodology with five antifungal agents (14). The Neo-Sensitabs assay (A/S Rosco Diagnostica, Taastrup, Denmark) utilizes a 9-mm-diameter (1-mm thickness) tablet for the antifungal susceptibility testing of yeasts and other fungal pathogens with several antifungal agents (Neo-Sensitabs user's guide; A/S Rosco Diagnostica) (2). This assay has been favorably compared with the broth microdilution CLSI M27-A2 method (3) for testing yeasts (1, 20, 23) and, more recently, for mold isolates with posaconazole and amphotericin B (13). Amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole tablets (A/S Rosco Diagnostica) and 6-mm amphotericin B, itraconazole, and voriconazole disks (Abtek Biologicals Ltd., Liverpool, United Kingdom) are available in Europe but not in the United States.The purpose of this study was to compare the Neo-Sensitabs tablet assay to both the CLSI reference broth microdilution (document M38-A) assay and the newly developed mold disk diffusion method for testing the susceptibility of 183 molds (Table 1) to five antifungal agents (amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole). The evaluation included the following determinations: (i) the determination of reference MICs and minimum effective concentrations (MECs; for caspofungin only) of the five agents by the CLSI broth microdilution M38-A method (4, 8, 18), (ii) the determination of inhibition zone diameters (in millimeters) by both disk an...

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Section: Resultsmentioning

confidence: 99%

Comparison of Neo-Sensitabs Tablet Diffusion Assay with CLSI Broth Microdilution M38-A and Disk Diffusion Methods for Testing Susceptibility of Filamentous Fungi with Amphotericin B, Caspofungin, Itraconazole, Posaconazole, and Voriconazole

Espinel-Ingroff

Cantón

2008

J Clin Microbiol

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“…Recent studies suggest that the agar diffusion method is comparable with the broth dilution method for MIC determination. This method is effective not only against bacteria (Andrews et al, 1991) but also against fungi (Serrano et al, 2004;Lopez-Oviedo et al, 2006).…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Antibacterial and Antifungal Activities of Different Parts ofTagetes patula.: Preparation of Patuletin Derivatives

Faizi

Siddiqi

Bano

et al. 2008

Pharmaceutical Biology

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The current study evaluates the antibacterial and antifungal activities of extracts from different parts of Tagetes patula Linn. (Asteraceae), reported for the first time in a single set of experiments. In the preliminary assay, the methanol extract of the flower (JFM) was found to possess antimicrobial activity against a number of bacteria with inhibition zone diameters ranging from 9 to 20 mm, the bioassay-guided fractionation of which led to the isolation of a flavonoid patuletin (3) in high yield as the active antibacterial principle with minimum inhibitory concentration (MIC) value of 12.5 µg/disk against Corynebacterium spp., Staphylococcus spp., Streptococcus spp., and Micrococcus luteus. Its glucoside, patulitrin (4), was found to be weakly active, except against Staphylococcus saprophyticus, Streptococcus fecalis, and Streptococcus pyogenes with inhibition zone diameters of 11, 16, and 12 mm, respectively. The cinnamate derivative (3b) of 3 showed antibacterial activity comparable with the parent flavonoid with a MIC value of 50 µg/disk against Corynebacterium spp., whereas benzoate derivative (3a) was found to be devoid of any activity; both the derivatives are new compounds. Moreover, the longchain alcohol 5, which displayed antibacterial activity in the preliminary testing, was obtained in large quantity directly from the petroleum ether extract of the involucre of the flowers.

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“…Both commercial methods have been favorably evaluated for MIC determination of voriconazole, amphotericin B, and itraconazole against Aspergillus spp. and other molds (1,10,20,21,26). More recently, Etest and disk methodology have been applied as alternative methods for testing posaconazole against yeasts (23) and molds (14,17).…”

mentioning

confidence: 99%

Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with Posaconazole and Amphotericin B

Espinel-Ingroff

2006

J Clin Microbiol

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A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

Abstract: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

Cited by 64 publications

References 30 publications

Comparison of Neo-Sensitabs Tablet Diffusion Assay with CLSI Broth Microdilution M38-A and Disk Diffusion Methods for Testing Susceptibility of Filamentous Fungi with Amphotericin B, Caspofungin, Itraconazole, Posaconazole, and Voriconazole

Comparison of Neo-Sensitabs Tablet Diffusion Assay with CLSI Broth Microdilution M38-A and Disk Diffusion Methods for Testing Susceptibility of Filamentous Fungi with Amphotericin B, Caspofungin, Itraconazole, Posaconazole, and Voriconazole

Antibacterial and Antifungal Activities of Different Parts ofTagetes patula.: Preparation of Patuletin Derivatives

Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with Posaconazole and Amphotericin B

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