A Comparative Study of the Three Cucumber Cultivars Using Fluorescent Staining and Fluorescence In Situ Hybridization

Abstract

Summary A molecular cytogenetic survey of Cucumis sativus L. revealed a chromosomal polymorphism among 2 European pickling cultivars of 'Borszczagowski' and 'Monastyrski', and one Japanese cultivar of 'Zibai' by fluorescent banding method with chromomycin A 3 (CMA) and 4Ј,6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with the probes of 5S, 45S rDNA and telomere repeat sequences. Heterochromatic banding polymorphism was found in some chromosomes with CMA and DAPI staining, whereas no chromosomal polymorphism was found in FISH. Thus, the FISH allowed us to identify all of homologous chromosomes in each cucumber cultivars, while CMA and DAPI banding showed an easy detection of the polymorphism in cucumber chromosomes, even in close related cultivars between European pickling cultivars.

Fluorescent Chromosome Banding Patterns in Six Species of <i>Abies</i>, Pinaceae

“…After about 10 d, the primary roots growing straight were collected and treated with 0.05% colchicine for 10 h, fixed in a fixative mixed with ethanol-acetic acid-chloroform (2 : 1 : 1) and then stored in a deep freezer until use. Fluorescent banding using CMA and 4′,6-diamidino-2-phenylindole (DAPI) was adapted to plant chromosomes by Schweizer (1976) and is effective for karyotype analysis in other various plant species (Hoshi et al 2008, Urdampilleta et al 2008, Zaman and Alam 2009, Begum et al 2010, Fawzia and Alam 2011, Hoshi et al 2011, Shahla and Alam 2011, Shirakawa et al 2012, Kuroki et al 2013. The fluorescent banding procedure simplified and adapted for conifer chromosome analysis by Kondo and Hizume (1982) was used.…”

Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review

“…Intraspecific variation regarding the presence and absence of ITR signals has been reported in a relatively low number of specie: the gymnosperm Zamia furfuracea (Zamiaceae, [7,37]) and the angiosperms Beta vulgaris (Amaranthaceae, [7,104]), Brassica oleracea (Brassicaceae [60,102]), Cestrum parqui (Solanaceae, [26,87]), Hordeum vulgare (Poaceae, Congeneric species may differ in the presence or absence of ITR signals as observed in unrelated groups from 16 families. This is illustrated in Asteraceae, where intrageneric polymorphisms have been detected in Achillea, Anacyclus, Anthemis, Cladanthus, Nassauvia, and Sonchus [69,82], Alstroemeriaceae (Alstroemeria, [31,44]), Brassicaceae (Brassica, [60]), Solanaceae (Cestrum, [26,87]), Rutaceae (Citrus, [92,93]), Poaceae (Colpodium, Hordeum, [47,61,94,95]), Cucurbitaceae (Cucumis, [98,99]), Orchidaceae (Dendrobium, [41]), Cyperaceae (Eleocharis, [48,97]), Lentibulariaceae (Genlisea, [27]), Cannabaceae (Humulus, [100,101]), Juncaceae (Luzula, [96]), Amaryllidaceae (Nothoscordum, Prospero, [18,23,86]), Rosaceae (Rosa, [62,103]), Fabaceae (Senna, Vicia, [7,45,74]), and Commelinaceae (Tradescantia, [6,103]…”

Next generation sequencing and omics in cucumber ( Cucumis sativus L.) breeding directed research

“…In this map they located 14 chromosome arm-specific FISH fosmid clones. At the same time, the chromosomal polymorphism among the different cucumber lines has also been analyzed mainly by base-specific fluorochrome stain- ing and FISH methods [64][65][66]. Additionally, the heterochromatin polymorphism among the 14 lines was also confirmed by DAPI and CMA staining [67].…”

“…A diagrammatic representation of the cytogenetic locations of tandem repeats (5S, 45S rDNA, telomere, Type III, Type IV, single gene (Xet), fosmid (AOX) and BACs from cucumber B10 line corresponding numbering by Yang et al[51] (A)[64][65][66]72]. Hybridized to centromeric region with signals using BAC clones 067-J12 (B) and 019-E12 (C).…”