A Comparative Study of the Biocompatibility of Two Root-End Filling Materials in Rat Connective Tissue

Abstract

The aim of the present study was to examine the short-term biocompatibility of Endosequence Root Repair Material (ERRM) paste and white Mineral Trioxide Aggregate MTA by implanting them into polyethylene tubes in the subcutaneous connective tissue of rats. twenty five male Wistar rats, 3-4 months old, weighing 300-350 g, were used. The tubes were implanted dorsally into the subcutaneous connective tissues of the rats. Five animals were sacrificed at five examination time points: 1, 3, 5, 7 and 15 days. The connective tissues containing the implants were excised. These sections were studied qualitatively and quantitatively using a light microscope. An average value for each group was obtained by averaging the sum of all inflammatory cells counted in 10 randomly selected, separate areas. For the ERRM group: There was a significant increase in the number of inflammatory cells on days 1-3 and on days 5-7 (P ≤ 0.003 and P ≤ 0.024). In the WHITE MTA group, the mean values of the sum of the inflammatory cells during the periods 1-3 days and 5-7 days were statistically significant (P ≤ 0.001 and P ≤ 0.044, respectively) and the XILOPERCHA group: Difference was observed significant in the value of the sum of inflammatory cells during the period of 3-5 days (P ≤ 0.05). According to the results it can be concluded that both, ERRM as MTA, caused an inflammatory reaction, which decreased over time; suggesting that both materials are biocompatible; showing however the presence of a higher organization of collagen fibers around the implants of ERRM.

Bioactivity analysis of calcium silicate‐based sealers and repair cements on the phenotype and cytokine secretion profile of CD14+ monocytes: An ex vivo study

“…Among the calcium silicate‐based repair cements, Biodentine, based on tricalcium silicate, has been available since 2010 and has similar properties to dentine, but with better clinical handling and shorter setting time than mineral trioxide aggregate (de Sousa Reis et al, 2019). TotalFill BC Fast‐Set Putty is another calcium silicate‐based repair cement released in 2016; it has a premixed putty consistency that helps improve its handling characteristics and setting time compared with its predecessor TotalFill BC Putty (Gutierrez‐Cantú et al, 2019; Koutroulis et al, 2019; Mahgoub et al, 2019).…”