A comparative study of the refractive index of silk protein thin films towards biomaterial based optical devices

Bucciarelli, Alessio; Mulloni, Viviana; Maniglio, Devid; Pal, Raktim; Yadavalli, Vamsi K.; Motta, Antonella; Quaranta, A.

doi:10.1016/j.optmat.2018.02.058

Cited by 61 publications

(50 citation statements)

References 45 publications

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“…They must also have adequate mechanical properties, decompose in response to the formation of new tissues, promote the spread of nutrients and metabolites, adhere and integrate with surrounding native tissues, and be able to adequately fill damaged areas (Freyman et al., 2001; M. Liu et al., 2017). Several natural matrices have been adopted in tissue engineering (silk fibroin, keratin, collagen, alginate and several polysaccharides) among them collagen is a versatile material that can be used to produce hydrogels, membranes, and porous scaffolds (Bucciarelli, Chiera et al., 2019; Bucciarelli et al., 2018, 2017; Bucciarelli, Muthukumar et al., 2019; Yang et al., 2019). Collagen has been successfully used in a wide range of tissue engineering applications as a base material for scaffolding and has been proved to be biocompatible, biodegradable, permeable, and with a low immunogenicity, it can also be formed in a way to tune the construct porosity (Dong & Lv, 2016).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Been

Choi

Cho

et al. 2021

J Tissue Eng Regen Med

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Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low‐cell density. The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self‐healed in several weeks, but major injuries may eventually require surgery. In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time. In recent years, cell therapy, in which cells are directly transplanted, has attracted attention. In this study, a scaffold for implanting chondrocytes was prepared. The scaffold was made as a sponge using the eggshell membrane and agarose. The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability. However, scaffolds made of collagen only has poor mechanical properties. For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water‐soluble, and then mixed with agarose to prepare a scaffold. Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three‐dimensional porosity. The scaffold was examined for Fourier‐transform infrared, mechanical properties, biodegradability, and biocompatibility. In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated. The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Been

Choi

Cho

et al. 2021

J Tissue Eng Regen Med

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“…Due to the unique combination of properties, such as mechanical strength and toughness, biocompatibility, biodegradability, thermal stability, and easy processability, regenerated silk fibroin has been used as a functional biomaterial, when a positive interaction with living tissue is required. This has ranged from tissue engineering and regenerative medicine to biosensing . While a plethora of micro and nanoscale architectures of silk fibroin have been explored in the literature, films, fibers, microparticles, and gels, building larger, macroscale objects of fibroin has been challenging.…”

Section: Introductionmentioning

confidence: 99%

A Thermal‐Reflow‐Based Low‐Temperature, High‐Pressure Sintering of Lyophilized Silk Fibroin for the Fast Fabrication of Biosubstrates

Bucciarelli

Chiera

Quaranta

et al. 2019

Adv Funct Materials

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Solid fibroin is a bulk nonporous material that can be prepared with two methods: a liquid-gel-solid transition from a fibroin solution or a sintering procedure starting from silk powder. Both methods have their own disadvantages: the first requires several weeks and the process is size dependent; the second requires high temperatures. To overcome these limitations, a lowtemperature sintering procedure based on a thermal-reflow is proposed in this work to produce in fast-fashion monoliths of solid fibroin. Thermal-reflow is a well-known mechanism that takes place when the glass transition temperature of the material is lower than the temperature used to process it. Water plays an important role decreasing the glass transition temperature down to 40 °C. For the first time, a thermal reflow is conducted on lyophilized silk fibroin at 40 °C, associating to the water addition a high-pressure compression. To optimize the process, a full factorial design of experiment is used. The material is then studied in the crucial phases by digital scanning calorimetry, Fouriertransform infrared spectroscopy, and scanning electron microscopy. Finally, a mechanical characterization and a preliminary in vitro test are conducted.

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“…The sheets formed are stable in a wide range of solvents, and can be stored in air or water over several weeks without degradation. 27,33 The cuts have a high structural fidelity and spatial resolution demonstrating the scalability and accuracy of this photolithographic process to form micropatterns over large areas. SEM imaging shows ordered patterns of various complexities over a large area (cm scale) of flexible fibroin sheet (Figure 2b-f).…”

Section: Fabrication Of Flexible Silk Fibroin Kirigamimentioning

confidence: 99%

“…[31][32] Here, microfabrication of flexible, optically transparent kirigami films is realized using a light-reactive silk protein with a rapid, scalable process. 26,33 Material subtraction or cutting is photolithographically accomplished in a single step process. The solution of photocrosslinkable (silk) fibroin in hexafluoroisopropanol was crosslinked by exposure through a photomask, resulting in complex patterns (Figure 1).…”

Section: Fabrication Of Flexible Silk Fibroin Kirigamimentioning

confidence: 99%

“…This was consistent with 12 previous observations in which the biodegradability of fibroin based flexible devices can be tuned by controlling the degree of crosslinking and film thickness. 26,33,49 Stretchable devices and biointerfaces with precisely engineered lifetimes can therefore be fabricated using the kirigami films, which can be useful as flexible biomimetic cellular constructs for tissue regeneration, drug delivery platforms, and biosensors.…”

Section: Evaluation Of Biocompatibility and Degradationmentioning

confidence: 99%

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Biofunctional Silk Kirigami With Engineered Properties

Pradhan

Ventura

Agostinacchio

et al. 2020

ACS Appl. Mater. Interfaces

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The fabrication of multifunctional materials that interface with living environments is a problem of great interest. A variety of structural design concepts have been integrated with functional materials to form biodevices and surfaces for health monitoring. In particular, approaches based on kirigami-inspired cuts can engineer flexibility in materials through the creation of patterned defects. Here, the fabrication of a biodegradable and biofunctional "silk kirigami" material is demonstrated. Mechanically flexible, free-standing, optically transparent, large-area biomaterial sheets with precisely defined and computationally designed microscale cuts can be formed using a single-step photolithographic process. Using modeling techniques, it is shown how cuts can generate remarkable "self-shielding" leading to engineered elastic behavior and deformation. As composites with conducting polymers, flexible, intrinsically electroactive sheets can be formed.Importantly, the silk-kirigami sheets are biocompatible, can serve as substrates for cell culture, and be proteolytically resorbed. The unique properties of silk kirigami suggest a host of applications as transient, "green", functional biointerfaces, and flexible bioelectronics.

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A comparative study of the refractive index of silk protein thin films towards biomaterial based optical devices

Cited by 61 publications

References 45 publications

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

A Thermal‐Reflow‐Based Low‐Temperature, High‐Pressure Sintering of Lyophilized Silk Fibroin for the Fast Fabrication of Biosubstrates

Biofunctional Silk Kirigami With Engineered Properties

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