A Comparative Study of the Growth Cycles of Different Members of the Psittacosis Group in Different Host Cells

Litwin, Jack; Officer, Julius E.; Brown, Arthur; Moulder, James W.

doi:10.1093/infdis/109.3.251

Cited by 41 publications

(33 citation statements)

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“…One growth cycle appeared to be complete in about 30 h at 35 "C; this is similar to the growth cycle time of other C. psittdci strains in tissue culture (Litwin, 1959;Litwin et al, 1961). The optimum incubation time for counting inclusions was 26 to 30 h; between these times the inclusions were large enough to be seen readily, but little release of agent from infected cells had occurred; detection of inclusions was also aided by the increase in cell size occurring after irradiation.…”

Section: Discussionsupporting

confidence: 65%

Infectivity Titration of Guinea-pig Inclusion Conjunctivitis Agent in Irradiated McCoy Cells

Griffiths¹,

Ainsworth²,

Pearce³

1976

Journal of General Microbiology

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Estimation of the infectivity of the agent of guinea-pig inclusion conjunctivitis for irradiated McCoy cells, assayed as inclusion-forming units, was influenced by the age of cells after irradiation, the maturation time of the inclusions, the centrifugal force and the centrifugation temperature. Agent passaged through irradiated McCoy cells or guinea-pig conjunctivae showed a greater capacity to infect irradiated McCoy cells without centrifuging than agent grown in a chick embryo. The nature of the change and the mechanism of infectivity enhancement by centrifuging are discussed.

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Section: Discussionsupporting

confidence: 65%

Infectivity Titration of Guinea-pig Inclusion Conjunctivitis Agent in Irradiated McCoy Cells

Griffiths¹,

Ainsworth²,

Pearce³

1976

Journal of General Microbiology

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“…However, the numerous growth curves which have been published for trachoma and other viruses of the psittacosis-lymphogranuloma group are so similar (e.g. Litwin, Officer, Brown & Moulder, 1961) that such differences are unlikely.…”

Section: Discussionmentioning

confidence: 99%

Observations on the growth of trachoma and inclusion blennorrhoea viruses in embryonate eggs

Reeve

Taverne

1963

J. Hyg.

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When trachoma and inclusion blennorrhoea viruses were titrated in chick embryo yolk sacs and the average day of death was plotted against dose of virus inoculated, sigmoid curves were obtained. Although all strains tested had the same growth rate, a given dose of some killed embryos more quickly than others. Strains killing most rapidly had the fewest elementary bodies per LD 50 and were the only strains to form inclusions in HeLa cells. During passage in the chick embryo three strains changed in their behaviour, killing embryos faster and acquiring the ability to form inclusions in HeLa cells.We wish to thank our colleagues Miss D. M. Graham and Dr W. A. Blyth for allowing us to quote some of their observations and Dr L. H. Collier and Dr G. G. Meynell for their help and criticism.

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“…Already in several laboratories workers have turned to electron microscopy in the hope that greater resolving power would be decisive in the vexed question of taxonomic relationships. Thinsection studies have been made of cells infected with the agents of meningopneumonitis (Gaylord, 1954;Tajima, Nomura & Kubota, 1957;Higashi, 1959), feline pneumonitis (Litwin, 1959), psittacosis (Litwin, Officer, Brown & Moulder, 1961) and trachoma (Mitsui et ul, 1958). Materials examined included tissues of infected animals and embryonated eggs, as well as inoculated cell cultures; but in terms of advancing previous knowledge the outcome has been inconclusive.…”

Section: Discussionmentioning

confidence: 99%

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

Armstrong

Valentine

Fildes

1963

Journal of General Microbiology

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SUMMARYPropagation of the trachoma agent in human cell cultures has made possible the observation by electron microscopy of sequential stages in the replication of the micro-organism. Scattered reticular foci with incomplete limiting membranes were present in the cytoplasm of HeLa cells 12 hr. after exposure to the infectious agent in high multiplicity. After 24 hr. the foci had coalesced to give a single reticular mass. Within it there appeared large and discrete developmental forms from which, by progressive subdivision and diminution in size, !mica1 elementary bodies were formed. The elementary bodies had a prominent limiting membrane, and a dense core within which a fibrillar nucleoid could be recognized. The controversial taxonomic status of the trachoma agent is considered briefly in the light of ultrastructural evidence.

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A Comparative Study of the Growth Cycles of Different Members of the Psittacosis Group in Different Host Cells

Cited by 41 publications

References 0 publications

Infectivity Titration of Guinea-pig Inclusion Conjunctivitis Agent in Irradiated McCoy Cells

Infectivity Titration of Guinea-pig Inclusion Conjunctivitis Agent in Irradiated McCoy Cells

Observations on the growth of trachoma and inclusion blennorrhoea viruses in embryonate eggs

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

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