A Comparative Study of the Histopathology and Immunohistochemistry of Pythiosis in Horses, Dogs and Cattle

Martins, Tessie Beck; Kommers, Gláucia D.; Trost, María Elisa; Inkelmann, Maria Andréia; Fighera, Rafael A.; Schild, Ana Lúcia

doi:10.1016/j.jcpa.2011.06.006

Cited by 54 publications

(59 citation statements)

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“…), in agreement with a previous study showing that differences are only numeric [19]. Conversely, Martins et al [13] showed that hyphal morphology (integrity) is normal when amongst eosinophils (necro-eosinophilic pattern) and disintegrated when associated with giant cells (granulomatous pattern). We speculate that, although the immunomodulatory properties of deferasirox were able to change the pattern of the cellular infiltrates observed in the treated groups, other factors such as poor bioavailability of the drug in the granulomatous lesions and different iron sources might not have reproduced the hyphal damage observed in vitro .…”

Section: Discussionsupporting

confidence: 90%

“…Lesion samples were fixed in formalin, embedded in paraffin and sections stained with hematoxylin and eosin (H&E) to evaluate the inflammatory cell infiltrate and Grocott’s methenamine silver nitrate (GMS) to evaluate hyphal morphology [13]. Two lesion samples were evaluated per animal, and two histological sections were performed in each slice.…”

Section: Methodsmentioning

confidence: 99%

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Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

et al. 2015

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Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

et al. 2015

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“…The main clinical alterations observed in all dogs were related to primary or secondary involvement of intestine, due to granulomatous inflammation unleashed by the agent (Salas et al 2009, Martins et al 2012, Galiza et al 2014, resulting in obstruction of the intestinal lumen. This is most commonly described clinical form of Pythiosis in dogs (Santurio et al 2006), though that may occur sporadically the cutaneous form of the disease (Howerth et al 1989, Torres Neto et al 2009, Pereira et al 2010.…”

Section: Discussionmentioning

confidence: 99%

“…A doença ocorre esporadicamente em cães no semiárido nordestino, entretanto deve ser incluída no diagnostico diferencial das doenças em cães com evolução crônica e proliferativa que acometem o sistema gastrintestinal, e frente a lesões cutâneas de difícil tratamento. (Fernandes et al 2012), affects several species of domestic animals, wildlife and humans (Camus et al 2004, Rakich et al 2005, Santurio et al 2006, Martins et al 2012. The equine species is the most affected, followed by the dogs (Leal et al 2001, Santurio et al 2006, Torres Neto et al 2009).…”

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“…In dogs the disease is characterized by the occurrence of cutaneous/subcutaneous and/or gastrointestinal lesions (Pereira et al 2010, Martins et al 2012, the latter being most common (Santurio et al 2006). The gastrointestinal tract lesions are characterized by thickening of the wall of the stomach and intestine (Trost et al 2009, Fernandes et al 2012, and may occur great masses with involvement by extension to the pancreas, mesentery, lymph nodes, liver, uterus and prostate (Miller 1985, Jaeger et al 2002, Grooters & Foil 2012.…”

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Pythiosis in dogs in the semiarid region of Northeast Brazil

et al. 2017

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RESUMO.-[Pitiose em cães na região semiárida do Nordeste, Brasil.] Descrevem-se os aspectos epidemiológicos, clínicos e patológicos de cinco casos de pitiose em cães na região semiárida do Nordeste. A doença ocorreu em cães com idade entre um e três anos, fêmeas e machos, de diferentes raças. Os cães eram criados em zona urbana, mas ocasionalmente eram levados para zona rural e tinham contato com áreas alagadas. Em um caso não havia informações no histórico sobre acesso à zona rural. Os principais sinais clínicos observados foram perda de peso, vômito, diarreia e tenesmo.Macroscopicamente havia espessamento da parede do intestino com massas firmes, irregulares, com áreas granulares amareladas entremeadas por tecido esbranquiçado, no duodeno, cólon e reto, que se estendia ao pâncreas e linfonodos, além de fígado e vagina. Na pele havia área de alopecia e ulcerações irregulares, algumas contendo pequenas cavitações com secreção serosanguinolenta. Microscopicamente havia inflamação piogranulomatosa e em um caso também havia necrose eosinofílica associada a imagens negativas tubuliformes pela hematoxilina e eosina, fortemente impregnadas pelo GMS e fracamente coradas pelo PAS. As hifas foram fortemente imunomarcadas pela imuno-histoquímica com anticorpo policlonal anti-Pythium insidiosum. A doença ocorre esporadicamente em cães no semiárido nordestino, entretanto deve ser incluída no diagnostico diferencial das doenças em cães com evolução crônica e proliferativa que acometem o sistema gastrintestinal, e frente a lesões cutâneas de difícil tratamento. We describe the epidemiological, clinical and pathological features of five cases of pythiosis in dogs in northeast semiarid. The disease occurred in dogs with age between one and three years, females and males of different breeds. The dogs were created in urban areas, but were occasionally taken to the countryside and then had contact with wetlands. The main clinical signs were weight loss, vomiting, diarrhea and tenesmus. Macroscopically there were intestinal wall thickening with irregular firm masses with yellowish granular areas interspersed with a whitish tissue, involving the duodenum, colon and rectum, extending to the lymph nodes and pancreas, and the vagina and liver. In the skin there were areas of alopecia and irregular ulcerations, some containing small cavitations with serosanguineous secretion. Microscopically there were pyogranulomatous inflammation and in one case also there were eosinophilic necrosis associated with negative images tubuliformes by hematoxylin and eosin, strongly stained by GMS and weakly by PAS. Hyphae were strongly marked by immunohistochemistry with polyclonal anti-Pythium insidiosum. The disease occurs sporadically in dogs in the semiarid Northeast, however should be included in the differential diagnosis of chronic diseases and proliferative aspect of the gastrointestinal system, and front skin lesions of difficult treatment.

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Coleman

2024

Techniques in Small Animal Wound Management

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A Comparative Study of the Histopathology and Immunohistochemistry of Pythiosis in Horses, Dogs and Cattle

Cited by 54 publications

References 22 publications

Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

Pythiosis in dogs in the semiarid region of Northeast Brazil

Specific Wounds

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