A Comparative Study of the Activity of Lysosomal and Main Metabolic Pathway Enzymes in Tissue Biopsies and Cultured Fibroblasts from <i>Dupuytren's </i>Disease and Palmar Fascia

Delbrück, A.; Reimers, E.; Schönborn, I

doi:10.1515/cclm.1981.19.9.931

Cited by 2 publications

(4 citation statements)

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“…In the Interpretation of the data, the biological variations between the single cell lines have to be taken into consideration. Reproducible results could not be expected unless the cell cultures linder investigation were matched aecording to their cell density; this is because, s shown previously (20), the metabolic activities of the ceils depend on the density of cell monolayers. Cells are the metabolic unit and the site of synthesis of the extracellular components of connective tissue.…”

Section: Discussionmentioning

confidence: 92%

“…The DNA content of cell cul-tures depends on the growth state of the individual cell line (fig. 3) and varies like the protein content or enzyme activities (20). Therefore, neither the protein nor DNA content seems suitable äs a reference for metabolic activities.…”

Section: Discussionmentioning

confidence: 99%

“…Uncontrolled tissue proliferation characterizes chronic diseases like rheumatoid arthritis, Dupuytren's diseases or liver drrhosis. Augmentation of cells and enhanced synthesis of extracellular matrix components occur simultaneously with the break-With the support of the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 54) I = 1. c. (20) down of the extracellular structure in the afflicted tissues. The pathomechanism that maintains and perpetuates the diseased state is so far unknown and may be based on disturbed cellular metabolism or on the effect of a variety of factors emerging from endogenous or exogenous processes occuring in the pathogenetic sequence of the respective diseases.…”

Section: Introductionmentioning

confidence: 99%

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Metabolism and Proliferation of Cultured Fibroblasts from Specimens of Human Palmar Fascia and Dupuytren'sContracture. The pathobiochemistry of connective tissue proliferation.

Delbrück¹,

Schröder²

1983

CCLM
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Cell Cultures from 11 Dupuytren's contracture and 6 normal palmar fascia specimens were established. The rates of sulphated glycosaminoglycan, collagen and JDNA synthesis by means of incorporation of labelled precursors ([ 35 S]sulphate, [ 3 H]proline, [ 3 ]thymidine) äs well äs the growth characteristics of the cell lines of both healthy and diseased were compared. The incorporation rates of [ 35 S]sulphate and [ 3 H]proline were found to be significantly higher in Dupuytren than in healthy palmar fascia-deriving cell lines. In contrast, no differeiices in cell growth or DNA synthesis could be demonstrated. The abnormal capacity to synthesize sulphated glycosaminoglycans and collagen is attributed to a permanent modulation of cell characteristics which can be propagated into cell culture. Stoffwechsel und Proliferation von Fibroblasten aus menschlicherPalmarfaszie und Dupuytren'schem Kontrakturgewebe in Zellkultur Zur Pathobiochemie der Bindegewebsproliferation, 2. Mitteilung Zusammenfassung: Kulturen von Zellen aus 11 Proben von Dupuytren'scher Kontraktur und 6 normalen Palmarfaszien wurden eingerichtet. Die Syntheseraten sulfatierter Gljfcosaminoglycane, von Kollagen und DNA wurden anhand der Einbauraten markierter Präcursoren ([ 35 S]Sulfat, [ 3 H]Prolin, [ 3 H]Thymidin), bestimmt und die Wachstumseigenschaften der Zell-Linien erkrankter und gesunder Gewebsproben verglichen. Die Einbauraten von [ 35 S]Sulfat und [ 3 H]Prolin waren in den Zellen von Dupuytren-Gewebe signifikant höher als in den Zell-Linien von Nprmalgewebe, während sich keine Unterschiede für die DNA-Synthese und das Wachstumsverhalten ergaben. Die Unterschiede in den Syntheseraten werden als Ausdruck einer permanenten Modulation der Zelleigenschaften interpretiert, die vom Gewebe in die Zellkultur übertragen werden können.

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Section: Discussionmentioning
confidence: 92%

Section: Discussionmentioning
confidence: 99%

Section: Introductionmentioning
confidence: 99%

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Metabolism and Proliferation of Cultured Fibroblasts from Specimens of Human Palmar Fascia and Dupuytren'sContracture. The pathobiochemistry of connective tissue proliferation.
Delbrück¹,
Schröder²
1983
CCLM
Self Cite
1
0
0
1
View full text Add to dashboard Cite
Cell Cultures from 11 Dupuytren's contracture and 6 normal palmar fascia specimens were established. The rates of sulphated glycosaminoglycan, collagen and JDNA synthesis by means of incorporation of labelled precursors ([ 35 S]sulphate, [ 3 H]proline, [ 3 ]thymidine) äs well äs the growth characteristics of the cell lines of both healthy and diseased were compared. The incorporation rates of [ 35 S]sulphate and [ 3 H]proline were found to be significantly higher in Dupuytren than in healthy palmar fascia-deriving cell lines. In contrast, no differeiices in cell growth or DNA synthesis could be demonstrated. The abnormal capacity to synthesize sulphated glycosaminoglycans and collagen is attributed to a permanent modulation of cell characteristics which can be propagated into cell culture. Stoffwechsel und Proliferation von Fibroblasten aus menschlicherPalmarfaszie und Dupuytren'schem Kontrakturgewebe in Zellkultur Zur Pathobiochemie der Bindegewebsproliferation, 2. Mitteilung Zusammenfassung: Kulturen von Zellen aus 11 Proben von Dupuytren'scher Kontraktur und 6 normalen Palmarfaszien wurden eingerichtet. Die Syntheseraten sulfatierter Gljfcosaminoglycane, von Kollagen und DNA wurden anhand der Einbauraten markierter Präcursoren ([ 35 S]Sulfat, [ 3 H]Prolin, [ 3 H]Thymidin), bestimmt und die Wachstumseigenschaften der Zell-Linien erkrankter und gesunder Gewebsproben verglichen. Die Einbauraten von [ 35 S]Sulfat und [ 3 H]Prolin waren in den Zellen von Dupuytren-Gewebe signifikant höher als in den Zell-Linien von Nprmalgewebe, während sich keine Unterschiede für die DNA-Synthese und das Wachstumsverhalten ergaben. Die Unterschiede in den Syntheseraten werden als Ausdruck einer permanenten Modulation der Zelleigenschaften interpretiert, die vom Gewebe in die Zellkultur übertragen werden können.
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“…Previous studies reported on alterations in the metabolic activity of the DD tissues 20. We identified the following glycolytic proteins: triosephosphate isomerase (TPI1), glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), pyruvate kinase isoenzymes M1/M2 (PKM2), and L ‐lactate dehydrogenase B chain (LDHB).…”

Section: Resultsmentioning
confidence: 99%

An integrated proteomics approach for studying the molecular pathogenesis of Dupuytren's disease
Pavelić
¹
,
Sedić
²
,
Hock
³

et al. 2008
The Journal of Pathology
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Dupuytren's disease (DD) is a fibromatosis characterized by non-malignant transformation of palmar fascia leading to permanent contraction of one or more fingers. Despite the extensive knowledge of its clinical pathogenesis, the aetiology of this disease remains obscure. In the present paper, we report for the first time on the proteomic profiling of diseased versus unaffected patient-matched palmar fasciae tissues from DD patients using two-dimensional gel electrophoresis coupled with mass spectrometry analysis. The herein identified proteins were then used to create the protein-protein interaction network (interactome). Such an integrated approach revealed the involvement of several different molecular processes related to DD progression, including extra- and intra-cellular signalling, oxidative stress, cytoskeletal changes, and alterations in cellular metabolism. In particular, autocrine regulation through ERBB-2 and IGF-1R receptors and the Akt signalling pathway have emerged as novel components of pro-survival signalling in Dupuytren's fibroblasts and thus might provide a basis for a new therapeutic strategy in Dupuytren's disease.

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A Comparative Study of the Activity of Lysosomal and Main Metabolic Pathway Enzymes in Tissue Biopsies and Cultured Fibroblasts from Dupuytren's Disease and Palmar Fascia

Cited by 2 publications

References 5 publications

Metabolism and Proliferation of Cultured Fibroblasts from Specimens of Human Palmar Fascia and Dupuytren'sContracture. The pathobiochemistry of connective tissue proliferation.

Metabolism and Proliferation of Cultured Fibroblasts from Specimens of Human Palmar Fascia and Dupuytren'sContracture. The pathobiochemistry of connective tissue proliferation.

An integrated proteomics approach for studying the molecular pathogenesis of Dupuytren's disease

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