A comparative study on effective cell disruption methods for lipid extraction from microalgae

Prabakaran, P.; Ravindran, A. David

doi:10.1111/j.1472-765x.2011.03082.x

Cited by 255 publications

(118 citation statements)

References 12 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…No decreasing amounts were detected. A similar result was reported by Prabakaran et al (2011). They compared the effect of the cell disruption method on lipid extraction for three microalgal species (Chlorella sp., Nostoc sp.…”

Section: Growth Parameter Valuesupporting

confidence: 77%

Physical Methods of Microalgal Biomass Pretreatment

Piasecka¹,

Krzemińska²,

Tys³

2014

International Agrophysics

View full text Add to dashboard Cite

The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

show abstract

Section: Growth Parameter Valuesupporting

confidence: 77%

Physical Methods of Microalgal Biomass Pretreatment

Piasecka¹,

Krzemińska²,

Tys³

2014

International Agrophysics

View full text Add to dashboard Cite

show abstract

“…High-pressure homogenization and ultrasonication were reported to enhance microalgal protein solubilization, the former being the most effective method (Gerde et al, 2013;Safi et al, 2014). Autoclaving was reported as an effective pretreatment to improve lipid extraction from microalgae (Prabakaran and Ravindran, 2011).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

et al. 2015

View full text Add to dashboard Cite

The current work reports protein extraction from Spirulina platensis cyanobacterial biomass in order to simultaneously generate a potential co-product and a biofuel feedstock with reduced nitrogen content. S. platensis cells were subjected to cell disruption by high-pressure homogenization and subsequent protein isolation by solubilization at alkaline pH followed by precipitation at acidic pH. Response surface methodology was used to optimize the process parameters -pH, extraction (solubilization/precipitation) time and biomass concentration for obtaining maximum protein yield. The optimized process conditions were found to be pH 11.38, solubilization time of 35 min and biomass concentration of 3.6% (w/w) solids for the solubilization step, and pH 4.01 and precipitation time of 60 min for the precipitation step. At the optimized conditions, a high protein yield of 60.7% (w/w) was obtained. The protein isolate (co-product) had a higher protein content [80.6% (w/w)], lower ash [1.9% (w/w)] and mineral content and was enriched in essential amino acids, the nutritious γ-linolenic acid and other high-value unsaturated fatty acids compared to the original biomass. The residual biomass obtained after protein extraction had lower nitrogen content and higher total non-protein content than the original biomass. The loss of about 50% of the total lipids from this fraction did not impact its composition significantly owing to the low lipid content of S. platensis (8.03%).

show abstract

“…Esto se debe a su capacidad productora de lípidos y a la rápida producción de biomasa, ya que en comparación con otras materias primas consideradas para producción de biodiesel como: aceites vegetales, grasa animal y desechos de aceite de cocina, estas presentan un suministro limitado, lo que impide la expansión de la producción del biocombustible (Prabakaran & Ravindran, 2011).…”

Section: Discussionunclassified

Identificación y caracterización molecular de cianobacterias tropicales de los géneros Nostoc, Calothrix, Tolypothrix y Scytonema (Nostocales: Nostocaceae), con posible potencial biotecnológico

Morales¹,

Villalobos²,

Rodríguez³

et al. 2017

URJ

View full text Add to dashboard Cite

Identification and molecular characterization of tropical cyanobacteria of the genus Nostoc, Calothrix, Tolypothrix and Scytonema (Nostocales: Nostocaceae), with possible biotechnological potential. Cyanobacteria or "Blue-Green" algae comprise a diverse cluster of prokaryotic photoautotrophs that share a high morphological and molecular abundance. Biotechnological advances on nitrogen fixation, bioremediation, pharmaceutical and nutritional value have been developed. Traditional identification based on morphology has been the most common applied technique, but it may be inaccurate because of the phenotypic plasticity that those organisms present. For this reason, molecular techniques had become robust tools for taxonomic positioning of tropical cyanobacterial isolates, associated with morphology identification. This study focuses on the morphological identification and molecular characterization of cyanobacteria isolated in different tropical environments of Costa Rica, for biotechnological prospects. Strains were grown in BG 0 -11 media, photographed under light microscope and classified at genera level. Molecular identification was carried by PCR and sequencing using DNA-dependent RNA polymerase (rpoC1) and small-subunit ribosomal RNA (16S rRNA) gen primers. Subsequently, a phylogenetic positioning analysis was performed by MAFFTv.7 alignment and maximum likelihood by raxMLGUI. Based on phenotypic characteristics, four genera of Nostocales were identified: Calothrix sp., Tolypothrix sp., Scytonema sp. and Nostoc sp. Molecular analysis shows a taxonomic grouping that is consistent with the observed morphology, with the 16S rDNA gene yielding robust and stable results. The identified genera are known for their nitrogen fixation capability: Nostoc and Calothrix have been used in maintaining fertility and soil recovering studies. Nostoc is known for its nutritional properties and biofuel production; a characteristic shared by Tolypothrix as well. The last genera; Scytonema, it is known for its pharmacological properties. With the successful identification of cyanobacteria strains isolated from tropical environments, it is possible to continue the study of genes responsible for properties of biotechnological interest and to evaluate their potential, with future perspectives of application and biodiversity conservation.Key words: Cyanobacteria, 16S rRNA, rpoC1, characterization molecular, morphological identification, biotechnological potential. RESUMEN:Las cianobacterias son procariotas fotoautótrofos, morfoló-gica y molecularmente diversas, en las cuales se han reportado avances biotecnológicos sobre fijación de nitrógeno, biorremediación y valor nutricional-farmacéutico. La identificación tradicional de cianobacterias basada en morfología puede ser imprecisa, por lo que las técnicas moleculares son una herramienta robusta para el posicionamiento taxonómico de estas cepas tropicales. Este estudio se enfoca en la identificación morfológica y caracterización molecular de cianobacterias de ambientes tropica...

show abstract

A comparative study on effective cell disruption methods for lipid extraction from microalgae

Cited by 255 publications

References 12 publications

Physical Methods of Microalgal Biomass Pretreatment

Physical Methods of Microalgal Biomass Pretreatment

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

Identificación y caracterización molecular de cianobacterias tropicales de los géneros Nostoc, Calothrix, Tolypothrix y Scytonema (Nostocales: Nostocaceae), con posible potencial biotecnológico

Contact Info

Product

Resources

About