A comparative study on microwave and routine tissue processing

Babu, T Mahesh; Narasimhan, Malathi; Magesh, KT

doi:10.4103/0970-9290.79975

Cited by 14 publications

(17 citation statements)

References 9 publications

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“…Cellular clarity, Colour intensity and overall Staining quality in our study was slightly higher for microwave method, in concordance with Babu et al 4 and Nangia et al 7 No statistically signiicant variation was observed between the two methods (p>0.05).…”

Section: Resultssupporting

confidence: 91%

“…The two methods did not show signiicant variation in terms of overall quality, consistent with the observations of numerous authors such as Rohr, Morales, Mathai, Devi, Babu. [1][2][3][4][5][6] Complete concordance of opinion with regard to quality of the two methods was seen in an average of 90.42% cases. Majority of tissue sections were of excellent quality (Score-4).…”

Section: Resultsmentioning

confidence: 81%

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Changing perspective on tissue processing - comparison of microwave histoprocessing method with the conventional method

2015

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 81%

Changing perspective on tissue processing - comparison of microwave histoprocessing method with the conventional method

2015

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“…It was observed that the shrinkage of the tissue bits was more pronounced with the DMWTP as compared to the CTP, but this did not affect the morphological quality of the tissues. This was consistent with the observations of other authors [10].…”

Section: Resultssupporting

confidence: 83%

“…Tissue bits with a size of 1* 1* 0.5 cm were taken [10], which included neoplastic and non-neoplastic lesions. Those tissues which were processed by microwaves and their paired tissues which were processed by conventional methods were grouped into groups I and II respectively.…”

Section: Exclusion Criteriamentioning

confidence: 99%

Domestic Microwave Versus Conventional Tissue Processing: A Quantitative and Qualitative Analysis

Pj²,

Bo³

2013

JCDR

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Background: Microwave irradiation has been tried as a replacement for the conventional tissue processing technique in histopathology laboratories for quite some time. Studies have shown that Domestic Microwave Tissue Processing (DMWTP) provides a faster delivery of the tissue sections with a morphology which is similar to that which is seen Conventional Tissue Processing (CTP). But many laboratories still confine the domestic microwave tissue processing method only to the handle selected specimens, for which urgent reports are needed. One of the probable reasons is that, understanding about the number of tissue sections which can be processed using a microwave oven at a time, with the appropriate quality, still remains unclear. Aim:The aim of this study was to quantitatively analyze the optimum number of samples that a domestic microwave could process at a time, as well as to qualitatively analyze the morphological outcome of those tissue sections with that of conventional processing. Materials and Methods:This study was approved by the research and ethical committee of Sree Balaji Medical College and Hospital. A total of 135 paired tissue sections were included in the study. Ten tissue sections (which are mentioned hereafter as A10) were processed in a domestic microwave and their paired 10 tissues were processed by a conventional method. Subsequently, the number of tissues which was to be processed was increased to B15, C20, D25, E30 and F35, after ascertaining that the morphological qualities of the previously processed tissue sections were satisfactory. Sections of 4 µm thickness were taken and they were stained by the Haematoxylin and Eosin method. The slides of the tissues which were processed by the microwave method and the conventional method were randomly numbered, for a blind study, which were independently evaluated by two observers. The qualities of slides were assessed, based on 4 parameters: the cytoplasmic details, the nuclear details, the tissue architecture and the staining characteristics. The statistical analysis was done by using SPSS 15.0. Results:The morphological outcomes (quality) of the DMWTPs were comparable to that of the CTPs, when the sample load (quantity) in the microwave oven was up to 25 samples.

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“…Whereas Babu et al . [ 9 ] and Kango and Deshmukh[ 10 ] found microwave processing to be better than conventional processing when nuclear detail is concerned.…”

Section: Discussionmentioning

confidence: 99%

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

Mishra¹,

Bandyopadhyay²,

Kumar³

et al. 2021

Journal of Pharmacy and Bioallied Sciences

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Introduction: Conventional tissue processing takes a lot of time, but microwave processing method though rapid, its quality of tissue texture and staining properties is still questionable. Therefore, we conducted this study to find out the reliability of histological sections in the microwave method. Aim and Objectives: The aim is to assess whether microwave tissue processing, which is a rapid technique, is comparable to conventional tissue processing in terms of quality and reliability of histological sections. Materials and Methods: A total of 80 formalin-fixed tissue samples were taken and were divided into two pieces. One subjected to conventional tissue processing and the other subjected to microwave tissue processing. The slides were coded and evaluated by three independent observers using various parameters. All the data were subjected to Chi-square test. Results: Both the process did not differ in their efficiency in overall nuclear details, overall cytoplasmic detail, occurrence of artifact and tissue architecture, nuclear staining, cytoplasmic staining, and red blood cell staining. Although the other observers did not find any statistical significance, according to observer 3, conventional processing proved to have marginally significantly better epithelial connective tissue interface than microwave processing ( P = 0.047). Conclusion: The microwave method of tissue processing is an economical procedure which reduces the obnoxious chemicals as well as the turnaround time.

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A comparative study on microwave and routine tissue processing

Abstract: The individual scores by different observers regarding the various parameters included in the study were statistically insignificant, the overall quality of microwave-processed and microwave-stained slides appeared slightly better than conventionally processed and stained slides.

Cited by 14 publications

References 9 publications

Changing perspective on tissue processing - comparison of microwave histoprocessing method with the conventional method

Changing perspective on tissue processing - comparison of microwave histoprocessing method with the conventional method

Domestic Microwave Versus Conventional Tissue Processing: A Quantitative and Qualitative Analysis

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

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