A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

Du, Jianghao; Zhu, Zhanyun; Yang, Junchang; Wang, Jia; Jiang, Xiaotong

doi:10.1186/s40494-021-00519-y

Cited by 11 publications

(6 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Demineralization steps can be avoided, and if protein solubilization is possible in a buffer compatible with mass spectrometry (e.g., ammonium bicarbonate or guanidinium hydrochloride), buffer exchange can be avoided, which mitigates protein loss. 137,146,153 Recently, bioactive films have been developed that allow "lab-on-plate" protein extraction directly from sample material such as artwork, which further simplifies extraction of surfaceavailable proteins. 154 In studies focusing on a small number of highly abundant proteins of interest, such as collagens in bone and parchment, additional simplifications can be made, even for mineralized samples.…”

Section: Methods Of Recoverymentioning

confidence: 99%

“…If the proteins are complex or are known to contain cysteines, reduction and alkylation steps are typically performed to irreversibly disrupt disulfide bonds. At this point, buffer exchange is frequently necessary to make the suspended or solubilized proteins compatible with downstream analysis, and different strategies for this are available including protocols based on the use of polyacrylamide gels, , filter-aided sample preparation (FASP), ,, gel-aided sample preparation (GASP), , single-pot solid-phase sample preparation (SP3), , or simply physical removal of the insoluble protein from the decalcification buffer in the case of collagen pseudomorphs . This is then typically followed by enzymatic digestion of the proteins into peptides, followed by peptide purification, typically using C18 resin (commercially available as StageTips [Pierce] and ZipTips [Millipore]).…”

Section: Ancient Proteinsmentioning

confidence: 99%

“…For nonmineralized samples, such as artist materials (binders, glues), mummified tissues, and parchment, simplifications to the protocol can be made. Demineralization steps can be avoided, and if protein solubilization is possible in a buffer compatible with mass spectrometry (e.g., ammonium bicarbonate or guanidinium hydrochloride), buffer exchange can be avoided, which mitigates protein loss. ,, Recently, bioactive films have been developed that allow “lab-on-plate” protein extraction directly from sample material such as artwork, which further simplifies extraction of surface-available proteins …”

Section: Ancient Proteinsmentioning

confidence: 99%

See 2 more Smart Citations

Paleoproteomics

2022

View full text Add to dashboard Cite

Paleoproteomics, the study of ancient proteins, is a rapidly growing field at the intersection of molecular biology, paleontology, archaeology, paleoecology, and history. Paleoproteomics research leverages the longevity and diversity of proteins to explore fundamental questions about the past. While its origins predate the characterization of DNA, it was only with the advent of soft ionization mass spectrometry that the study of ancient proteins became truly feasible. Technological gains over the past 20 years have allowed increasing opportunities to better understand preservation, degradation, and recovery of the rich bioarchive of ancient proteins found in the archaeological and paleontological records. Growing from a handful of studies in the 1990s on individual highly abundant ancient proteins, paleoproteomics today is an expanding field with diverse applications ranging from the taxonomic identification of highly fragmented bones and shells and the phylogenetic resolution of extinct species to the exploration of past cuisines from dental calculus and pottery food crusts and the characterization of past diseases. More broadly, these studies have opened new doors in understanding past human−animal interactions, the reconstruction of past environments and environmental changes, the expansion of the hominin fossil record through large scale screening of nondiagnostic bone fragments, and the phylogenetic resolution of the vertebrate fossil record. Even with these advances, much of the ancient proteomic record still remains unexplored. Here we provide an overview of the history of the field, a summary of the major methods and applications currently in use, and a critical evaluation of current challenges. We conclude by looking to the future, for which innovative solutions and emerging technology will play an important role in enabling us to access the still unexplored "dark" proteome, allowing for a fuller understanding of the role ancient proteins can play in the interpretation of the past.

show abstract

Section: Methods Of Recoverymentioning

confidence: 99%

Section: Ancient Proteinsmentioning

confidence: 99%

Section: Ancient Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Paleoproteomics

2022

View full text Add to dashboard Cite

show abstract

“…Paint adhesive samples scraped from the surface of the painting were pooled together, and proteins were extracted by 6 M urea (final concentration of 20 mg/ml), followed by overnight vortex mixing and 15 min centrifugation at 11,000g. Urea is a valuable alternative to extract proteinaceous binders (Du et al 2021). Twelve two-fold serial dilutions (4.7-2400 μg) in 6 M urea were prepared from the supernatant and were analysed by the dot blot immunoassay according to Cattò et al (2017).…”

Section: Resultsmentioning

confidence: 99%

Dot blot immunochemical and infrared analyses of the adhesive layer applied to the painting Imago Pietatis by Domenico Morone

Cattò

Parodi²,

Chiodelli³

et al. 2022

Ann Microbiol

View full text Add to dashboard Cite

Purpose To investigate the nature of the materials used in the adhesive layer of the Imago Pietatis painting (end of the fifteenth century—beginning of the sixteenth century) by Domenico Morone as a prerequisite for its restoration. Methods Micro-FTIR spectra of the animal glue and a polished cross-section were acquired by a Jasco IRT3000 spectrometer, equipped with a 32× Cassegrain objective. A dot blot immunoassay was used to characterise a minor component of the adhesive layer. Results Micro-FTIR was used as an effective diagnostic tool to detect the major component of the adhesive layer and the binder of the paint. Despite the ageing, the complex matrix and the micro-size of the sample, using a dot blot immunoassay, it was possible to quantify 3.7 ± 2.0 ng of ovalbumin per microgram of sample (corresponding to 0.004 ± 0.002% of the weight). Conclusions The findings were in line with conservation practices described in the old treatises, confirming the correct interpretation of the adhesive layer compounds added to the painting and suggesting for the cleaning the use of an anionic water-soluble surfactant highly effective in the removing of proteinaceous materials.

show abstract

“…The protein extraction efficiencies of the selected agents were quantitatively determined by bicinchoninic acid method, and then processed by multivariate analysis of variance. The authors claim that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride was significantly higher than the other five agents, with less damage to the protein structure during the extraction procedure [60].…”

Section: Invasive Techniquesmentioning

confidence: 99%

Research on the Organic Binders in Archaeological Wall Paintings

Casoli

2021

Applied Sciences

View full text Add to dashboard Cite

Wall painting realized using organic binders is the oldest form of parietal painting and precedes the birth of the affresco by about 20,000 years. This paper reports the results obtained from the main studies in the field of archaeological wall paintings. The attention was paid to the study of organic binders used for the application of the color, as well as on the instrumental techniques chosen to obtain such information. Different techniques can be used for the study of organic material in archeological paintings: non-destructive techniques, which can be applied directly in situ without sampling, and laboratory micro-invasive techniques for a more in-depth characterization. Among these, the chromatographic techniques represent a potential tool to acquire as much information as possible about chemical composition of binders.

show abstract

A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

Cited by 11 publications

References 90 publications

Paleoproteomics

Paleoproteomics

Dot blot immunochemical and infrared analyses of the adhesive layer applied to the painting Imago Pietatis by Domenico Morone

Research on the Organic Binders in Archaeological Wall Paintings

Contact Info

Product

Resources

About