A comparative study on the pore-size and filter type effect on the molecular composition of soil and stream dissolved organic matter

Denis, Marie; Jeanneau, Laurent; Pierson-Wickmann, Anne-Catherine; Humbert, Guillaume; Petitjean, Patrice; Jaffrézic, Anne; Gruau, Gérard

doi:10.1016/j.orggeochem.2017.05.002

Cited by 20 publications

(14 citation statements)

References 46 publications

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“…Further trials of universal inoculums across a range of aquatic environments would be beneficial in assessing these potential issues. This study suggests that filter size has relatively little impact on DOC concentration and DOM structure, supporting previous studies (Zsolnay, 2003;Bouillon et al, 2014;Denis et al, 2017). The choice of filter size for DOM sample storage, therefore, is relatively unimportant.…”

Section: Wider Implications Limitations and Future Worksupporting

confidence: 85%

“…Based on the lack of a significant difference between DOC concentrations in the treatments at the initial time point (Fig. 1), there is very little DOC contained between the 0.2 and 0.7 µm size range; this is supported by previous work (Zsolnay, 2003;Bouillon et al, 2014;Denis et al, 2017). Most DOM molecules are very small, less than ∼ 0.1 µm in size (Gustafsson and Gschwend, 1997), with very little in the 0.2 to 0.7 µm size range contributing to the overall DOC concentration.…”

Section: Doc Dynamicssupporting

confidence: 75%

“…This suggests that what small initial structural differences existed between the P2 and P7 treatments appeared unimportant to the overall dynamics of the DOM pool, which is supported by the lack of difference in initial DOC concentrations ( Fig. 1) and previous work (Zsolnay, 2003;Bouillon et al, 2014;Denis et al, 2017).…”

Section: Doc Dynamicssupporting

confidence: 58%

“…The original set of truncated and quality-filtered reads were mapped back to the resulting OTUs to create an OTU table of read abundance per OTU per sample. Representative sequences for each OTU were aligned using the tool PyNAST (Caporaso et al, 2010a) and assigned to taxonomy using the RDP classifier (Wang et al, 2007) using QIIME version 1.7.0 scripts (Caporaso et al, 2010b) and Greengenes 2011 release as the reference database (DeSantis et al, 2006). Candidate OTUs that failed to align with more than 70 % similarity were discarded as putative artefacts.…”

Section: Bioinformaticsmentioning

confidence: 99%

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Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

et al. 2018

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Abstract. Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized biolability assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtration strategies: 0.2 µm (filtered and inoculated), 0.7 µm (generally the most common DOM filter size) and 106 µm (unfiltered). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, the filtration strategy also affected the DOM degradation dynamics, including the δ13C signature. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 µm filtered incubations reaching this point the quickest. These results suggest that assays used to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtration strategy should also be taken into account when comparing results across biolability assays.

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Section: Wider Implications Limitations and Future Worksupporting

confidence: 85%

Section: Doc Dynamicssupporting

confidence: 75%

Section: Doc Dynamicssupporting

confidence: 58%

Section: Bioinformaticsmentioning

confidence: 99%

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Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

et al. 2018

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“…What is the impact of anaerobic conditions on this coupling? To answer these questions, the DIC and DOC concentrations were quantified by sampling the soil solutions moving freely in the soil macropores using zero-tension lysimeters [28] and the soil CO 2 efflux was monitored from the soil surface. This study took advantage of the long-term research program on SOM biogeochemistry [13,14,26,[29][30][31][32][33][34] at the Kervidy-Naizin catchment which is part of the AgrHys Critical Zone Observatory [35], within the French Critical Zone Observatory research infrastructure OZCAR [36] and can be considered as representative of lowland agricultural sites under temperate climate. In order to ascertain how the topography and water table dynamics control the coupling between F CO2 , DIC and DOC, sampling campaigns were performed fortnightly during one hydrological year from October to June, when water moves freely in the soil macropores leading to SOM destabilization through the export of gaseous and dissolved products.…”

Section: Introductionmentioning

confidence: 99%

Water Table Dynamics Control Carbon Losses from the Destabilization of Soil Organic Matter in a Small, Lowland Agricultural Catchment

et al. 2019

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The biogeochemistry of soil organic matter (SOM) is driven by a combination of stabilization and destabilization mechanisms. Among the various ways in which SOM is lost, soil moisture controls the leaching of dissolved organic and inorganic carbon (DOC and DIC) and CO2 fluxes (FCO2). The aim of this study was to investigate the impact of naturally occurring water table dynamics on the couplings between these three types of C losses. The DIC and DOC concentrations in the soil solutions and the FCO2 values at the soil surface were collected fortnightly over a nine-month period at four sampling points located along two topographic transects characterized by different water table dynamics. The water table depth, soil temperature and water-filled pore space (WFPS) were monitored at each site. Linear and nonlinear regressions were used to explore the couplings between C losses, WFPS and soil temperature. The dynamics of the water table seem to drive DOC solubilization, diffusion, and export mechanisms in addition to microbial processes and the equilibrium between DIC and CO2. The main descriptors of this water table dynamic were the residence time, return time and number of oscillations of the water table. Considering both transects, FCO2 was positively correlated with DOC, which highlights the importance of substrate accessibility for SOM mineralization. This paper emphasizes the importance of the water table dynamic for the coupling between SOM carbon losses.

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Challenges in quantifying and characterizing dissolved organic carbon: Sampling, isolation, storage, and analysis

et al. 2022

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Despite the advancements in analytical techniques, there are still great challenges and difficulties in accurately and effectively quantifying and characterizing dissolved organic carbon (DOC) in environmental samples. The objectives of this review paper are (a) to understand the roles and variability of DOC along the water continuum; (b) to identify the constraints, inconsistences, limitations, and artifacts in DOC characterization; and (c) to provide recommendations and remarks to improve the analytical accuracy. For the first objective, we summarize the four ecological and engineering roles of DOC along the water continuum from source water to municipal utility, including nutrients and energy sources, controlling the fates of micropollutants, buffering capacity, and treatability and precursors of disinfection byproducts. We also discuss three major challenges in DOC analysis, including spatial and temporal variations, degradability and stability, and unknown structures and formulas. For the second objective, we review the procedures and steps in DOC analysis, including sampling in diverse environmental matrices, isolation of DOC fraction, storage and preservation techniques, and analyses on bulk chemical characteristics. We list and discuss the available options and evaluate the advantages and disadvantages of each choice. Last, we provide recommendations and remarks for each stage: sampling, isolation, storage, and analysis.

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A comparative study on the pore-size and filter type effect on the molecular composition of soil and stream dissolved organic matter

Abstract: et al.. A comparative study on the pore-size and filter type effect on the molecular composition of soil and stream dissolved organic matter.

Cited by 20 publications

References 46 publications

Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

Water Table Dynamics Control Carbon Losses from the Destabilization of Soil Organic Matter in a Small, Lowland Agricultural Catchment

Challenges in quantifying and characterizing dissolved organic carbon: Sampling, isolation, storage, and analysis

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