Bahr Shebeen canal (BSC) and Khadraweya canal (KHC) are two Nilotic canals, the first is less polluted and the second is polluted. Oreochromis niloticus is the major fish species in the Nile and its branches, and consequently, this study is carried out on their biological parameters are studied to investigate the effect of pollution in those habitats. These included: various parameters of growth, mortality, survival rates, stomach-somatic index, gonado-somatic index, and hepato-somatic index. It was found that there is a significant decline in those parameters in the fish of KHC, as compared to BSC, which could be tied to pollution in that canal.