A Comparative Transcriptome Analysis of Volvariella volvacea Identified the Candidate Genes Involved in Fast Growth at the Mycelial Growth Stage

Abstract

The edible straw mushroom, Volvariella volvacea, is one of the most important cultivated mushrooms in tropical and sub-tropical regions. Strain improvement for V. volvacea is difficult because of the unknown mechanisms involved in its growth regulation and substrate utilization. A comparative physiological and transcriptomic study was conducted between two commercially available straw mushroom strains (v9 and v26) to explore their fast-growth regulation mechanism(s). The physiological study showed that V. volvacea v9 had a shorter growth cycle and higher biological efficiency (4% higher) than that in v26. At least 14,556 unigenes were obtained from the four cDNA libraries (two replicates per strain). Among them, the expression of 1597 unigenes was up-regulated while 1352 were down-regulated. Four heat-shock proteins were highly expressed in v9, showing that v9 has the better ability to handle stresses and/or environmental changes. Moreover, up to 14 putative transporter genes were expressed at a higher level in v9 than those in v26, implying that v9 has a better ability to transport nutrients or export xenobiotics efficiently. Our report allows to identify the candidate genes involved in the fast growth requirement of V. volvacea, which represents a valuable resource for strain improvement in this commercially important edible mushroom.

Edible mushrooms – perspectives and considerations

“…Volvariella volvacea (Bulliard ex Fries) Singer, 1951, also known as "rice mushroom", "straw fungus" or "Chinese mushroom", is a species found in tropical and subtropical climates (Liu et al, 2020). Its cultivation is believed to have originated in China, possibly before the 18th century (Chang, 1977), and has been used due to its high concentrations of nutrients and bioactive compounds with medicinal properties (Thuc et al, 2020).…”

Transcriptomics Analysis of Primordium Formation in Pleurotus eryngii

“…Transcriptome analysis of the three developmental stages of Wolfiporia cocos revealed that peroxisome, unsaturation of fatty acids, and degradation pathway were respectively prevalent in three stages [ 10 ]. A comparative transcriptomics study between two commercially available Volvariella volvacea strains, V9 and V26, identified various candidate genes involved in the fast growth requirement of V. volvacea , which provided a valuable resource for strain improvement of this commercially edible mushroom [ 11 ]. A. auricula has a stronger selenium accumulation ability, and comparative transcriptomics analysis between its mycelia collected from ordinary medium and medium containing 100 μg/g selenium identified the genes and metabolic pathways related to selenium accumulation [ 12 ].…”

Volvariella volvacea (Pluteaceae, Agaricales), presencia de un hongo con potencial biotecnológico en la Argentina

“…Volvariella volvacea (Bull.) Singer, también conocida como "seta del arroz", "hongo de la paja" o "Chinese mushroom" es una especie de climas tropicales y subtropicales (Liu et al, 2020). Su cultivo se habría originado en China, presumiblemente antes del siglo XVIII (Chang, 1977), aprovechando sus elevadas concentraciones de nutrientes y compuestos bioactivos con propiedades medicinales (Chen et al, 2019;Thuc et al, 2020).…”