A comparison between diamine oxidases from human post-heparin blood serum, pregnancy blood serum and amniotic fluid

Abstract

Diamine oxidase (DAO) was purified from blood serum after intravenous heparin injection, from pregnancy blood serum and amniotic fluid. On DEAE-Sephadex, DAO from amniotic fluid as well as from pregnancy blood serum was separated in two active fractions with molecular weights of 245,000 and 485,000, respectively. Post-heparin blood serum contained only one active DAO fraction with a molecular weight of 185,000. The purified enzymes showed similar enzymatic properties.

Mammalian Cu‐containing amine oxidases (CAOs): New methods of analysis, structural relationships, and possible functions

“…, 1975, Crabbe eta/. , 1976, Tufvesson, 1978a, 1978b, Lin eta!. , 1981, Gahl eta!., 1982, Suzuki and Matsumoto, 1987a, 1987b, Morel et at.…”

“…M r values for CAOs as determined by gel fi ltration have ranged from 670000 to 180000 (Holt! a et al, 1975, Tufvesson, 1978a, 1978b, Gahl and Pilot, 1982. Suzuki and Matsumoto, 1987a, 1987b .. .…”

“…During exponential g rowth only small amounts of polyamines a re excreted except fo r putrescine, whe reas under growth arrest conditions, spermine is converted to spermidine, which is actively excreted together with putrescine (Melvin and Kcir, 1978a, 1978b, McCormick, 1978lace a nd Keir, 1981,Wallace, I987,Tjandrawinata et a /., 1994, Tjandrawinata andByus, 1995). lnterconversion ofpolyami nes occurs by acetylation followed by degradation of the acetylated polyamines by the ubiquito us enzyme polyamine oxidase (PAO) ( Fig.…”

Diamine Oxidase Induces Neurite Outgrowth in Chick Dorsal Root Ganglia by a Nonenzymatic Mechanism

“…In the placenta, it localizes to the decidua at the boundary between the maternal (decidual) and fetal (trophoblastic) tissues (Gunther and Glick, 1967;Lin et al, 1975). It is also likely that the placenta is the source of the large amounts of DAD that are present in the plasma throughout the course of pregnancy (Tufvesson, 1978). DAD activity increases in rat intestinal epithelium both during maturation and following injury (Luk et a!., 1980).…”